Identification and validation of ferroptosis-related genes and immune cell infiltration in thyroid associated ophthalmopathy

Chen, Sainan; Diao, Jiale; Yue, Zifan; Wei, Rui-Li

doi:10.3389/fgene.2023.1118391

Cited by 5 publications

(3 citation statements)

References 31 publications

(32 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Studies have shown its association with thyroid-associated ophthalmopathy, gastric cancer, and susceptibility to glaucoma. Furthermore, it can function as a ceRNA to influence the severity of schizophrenia ( 53 - 55 ). This RNA acts as an oncogene in bladder cancer and breast cancer, but its role in ACC is not yet clear.…”

Section: Discussionmentioning

confidence: 99%

LncRNA ZFHX4-AS1 as a novel biomarker in adrenocortical carcinoma

Chen,

Li,

et al. 2024

Transl Androl Urol

View full text Add to dashboard Cite

Background Adrenocortical carcinoma (ACC) is a rare and highly aggressive malignant tumor. Currently, there is a lack of reliable prognostic markers in clinical practice. Extensive research has shown that long non-coding RNA (lncRNA) are critical factors in the initiation and progression of cancer, closely associated with early diagnosis and prognosis. Previous studies have identified that ZFHX4 antisense RNA 1 (ZFHX4-AS1) is aberrantly expressed in various cancers and is associated with poor outcomes. This study investigates whether ZFHX4-AS1 affects the prognosis of ACC patients and, if so, the potential mechanisms involved. Methods In this study, utilizing four multi-center cohorts from The Cancer Genome Atlas (TCGA) program and Gene Expression Omnibus (GEO), we validated the prognostic capability of ZFHX4-AS1 in ACC patients through Kaplan-Meier survival analysis, cox regression models, and nomograms. Then, we explored the biological functions of ZFHX4-AS1 using gene set enrichment analysis (GSEA), competing endogenous RNA (ceRNA) networks, and analyses of somatic mutations and copy number variation (CNV). Finally, in vitro experiments were conducted to further validate the impact of ZFHX4-AS1 on proliferation and migration capabilities of ACC cell lines. Results Survival analysis indicated that patients in the high ZFHX4-AS1 expression group of ACC had worse prognosis. Cox regression analyses suggested that ZFHX4-AS1 levels were independent risk factors for prognosis. Subsequently, we constructed nomograms based on clinical features and ZFHX4-AS1 levels, demonstrating good predictive performance under the time-dependent receiver operating characteristic (ROC) curve. Analysis based on somatic mutations and CNV revealed that CTNNB1 and 9p21.3-Del drove the expression of ZFHX4-AS1. Cell Counting Kit-8 (CCK-8), colony formation, and Transwell assays confirmed that knockdown of ZFHX4-AS1 inhibited proliferation and migration of ACC cells. Conclusions This study demonstrates that ZFHX4-AS1 has a reliable predictive value for the prognosis of ACC patients and is a promising biomarker.

show abstract

Section: Discussionmentioning

confidence: 99%

LncRNA ZFHX4-AS1 as a novel biomarker in adrenocortical carcinoma

Chen,

Li,

et al. 2024

Transl Androl Urol

View full text Add to dashboard Cite

show abstract

“…According to recent research, the co-expression relationship of differentially expressed lncRNA and extracellular matrix related mRNA implies that lncRNA may be involved in the control of extracellular matrix remodeling in TAO orbital fat/connective tissue (31). Furthermore, the lncRNA-miRNA-mRNA network was built using high-throughput sequencing of orbital tissue, which was likewise linked to the etiology of TAO (32). LINC01140 has been shown in cell tests to control macrophage M2 polarization (33) and lessen their inflammatory response (34).…”

Section: Discussionmentioning

confidence: 99%

Identification of optimal feature genes in patients with thyroid associated ophthalmopathy and their relationship with immune infiltration: a bioinformatics analysis

Xiong,

Wang,

et al. 2023

Front. Endocrinol.

View full text Add to dashboard Cite

BackgroundThyroid associated ophthalmopathy (TAO) is an organ-specific autoimmune disease that has a significant impact on individuals and society. The etiology of TAO is complicated and poorly understood. Thus, the goal of this study was to use bioinformatics to look into the pathogenesis of TAO and to identify the optimum feature genes (OFGs) and immune infiltration patterns of TAO.MethodsFirstly, the GSE58331 microarray data set was utilized to find 366 differentially expressed genes (DEGs). To find important modular genes, the dataset was evaluated using weighted gene coexpression network analysis (WGCNA). Then, the overlap genes of major module genes and DEGs were further assessed by applying three machine learning techniques to find the OFGs. The CIBERSORT approach was utilized to examine immune cell infiltration in normal and TAO samples, as well as the link between optimum characteristic genes and immune cells. Finally, the related pathways of the OFGs were predicted using single gene set enrichment analysis (ssGSEA).ResultsKLB, TBC1D2B, LINC01140, SGCG, TMEM37, and LINC01697 were the six best feature genes that were employed to create a nomogram with high predictive performance. The immune cell infiltration investigation revealed that the development of TAO may include memory B cells, T cell follicular helper cells, resting NK cells, macrophages of type M0, macrophages of type M1, resting dendritic cells, active mast cells, and neutrophils. In addition, ssGSEA results found that these characteristic genes were closely associated with lipid metabolism pathways.ConclusionIn this research, we found that KLB, TBC1D2B, LINC01140, SGCG, TMEM37, and LINC01697 are intimately associated with the development and progression of TAO, as well as with lipid metabolism pathways.

show abstract

“…Coincidentally, it was reported that Graves’ hyperthyroidism could interfere with iron metabolism and elevate serum ferritin ( 16 ). In a recent study, several ferroptosis driver genes were found to exhibit relatively low expression levels in orbital tissues of TAO patients, indicating the abnormal iron metabolism in patients with TAO ( 17 ). However, whether the brain iron concentration of TAO patients exists abnormality remains unknown.…”

Section: Introductionmentioning

confidence: 99%

Increased brain iron in patients with thyroid-associated ophthalmopathy: a whole-brain analysis

Hu,

Zhou,

Fang

et al. 2023

Front. Endocrinol.

View full text Add to dashboard Cite

BackgroundTo investigate the whole-brain iron deposition alternations in patients with thyroid-associated ophthalmopathy (TAO) using quantitative susceptibility mapping (QSM).MethodsForty-eight patients with TAO and 33 healthy controls (HCs) were enrolled. All participants underwent brain magnetic resonance imaging scans and clinical scale assessments. QSM values were calculated and compared between TAO and HCs groups using a voxel-based analysis. A support vector machine (SVM) analysis was performed to evaluate the performance of QSM values in differentiating patients with TAO from HCs.ResultsCompared with HCs, patients with TAO showed significantly increased QSM values in the bilateral caudate nucleus (CN), left thalamus (TH), left cuneus, left precuneus, right insula and right middle frontal gyrus. In TAO group, QSM values in left TH were positively correlated with Hamilton Depression Rating Scale (HDRS) scores (r = 0.414, p = 0.005). The QSM values in right CN were negatively correlated with Montreal Cognitive Assessment (MoCA) scores (r = -0.342, p = 0.021). Besides that, a nearly negative correlation was found between QSM values in left CN and MoCA scores (r = -0.286, p = 0.057). The SVM model showed a good performance in distinguishing patients with TAO from the HCs (area under the curve, 0.958; average accuracy, 90.1%).ConclusionPatients with TAO had significantly increased iron deposition in brain regions corresponding to known visual, emotional and cognitive deficits. QSM values could serve as potential neuroimaging markers of TAO.

show abstract

Identification and validation of ferroptosis-related genes and immune cell infiltration in thyroid associated ophthalmopathy

Cited by 5 publications

References 31 publications

LncRNA ZFHX4-AS1 as a novel biomarker in adrenocortical carcinoma

LncRNA ZFHX4-AS1 as a novel biomarker in adrenocortical carcinoma

Identification of optimal feature genes in patients with thyroid associated ophthalmopathy and their relationship with immune infiltration: a bioinformatics analysis

Increased brain iron in patients with thyroid-associated ophthalmopathy: a whole-brain analysis

Contact Info

Product

Resources

About