Aims:
Bulk and single-cell RNA sequencing data were analyzed to explore the association
of stemness phenotype with dysfunctional anti-tumor immunity and its impact on clinical outcomes
of primary and relapse HCC.
Background:
The stemness phenotype is gradually acquired during cancer progression; however, it
remains unclear the effect of stemness phenotype on recurrence and clinical outcomes in hepatocellular
carcinoma (HCC).
Methods:
The stemness index (mRNAsi) calculated by a one-class logistic regression algorithm in
multiple HCC cohorts was defined as the stemness phenotype of the patient. Using single-cell profiling
in primary or early-relapse HCC, cell stemness phenotypes were evaluated by developmental potential.
Differential analysis of stemness phenotype, gene expression and interactions between primary
and recurrent samples revealed the underlying immune evasion mechanisms.
Results:
A significant mRNAsi association with HCC patient clinical outcomes was found. The high
and low mRNAsi groups had distinct tumor immune microenvironments. Cellular stemness phenotype
varied by cell type. Moreover, compared with primary tumors, early-relapse tumors had increased
stemness of dendritic cells and tumor cells and reduced stemness of T cells and B cells.
Moreover, in relapse tumors, CD8+ T cells displayed a low stemness state, with a high exhausted
state, unlike the high stemness state observed in primary HCC.
Conclusions:
The comprehensive characterization of the HCC stemness phenotype provides insights
into the clinical outcomes and immune escape mechanisms associated with recurrence.