Identification and up-regulation of three small heat shock proteins in summer and winter diapause in response to temperature stress in Pieris melete

Miano, Falak Naz; Jiang, Ting; Zhang, Jing; Zhang, Wanna; Peng, Yajun; Xiao, Hai

doi:10.1016/j.ijbiomac.2022.04.093

Cited by 3 publications

(2 citation statements)

References 48 publications

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“…Acting independently of ATP, they are the first line of cell defense, preventing the irreversible denaturation of substrate proteins, especially when cells are stressed [ 18 ]. Small HSPs have been extensively studied in diapausing insects, including the fruit flies Drosophila triauraria and D. melanogaster [ 23 , 81 ], the flesh fly, S. crassipalpis [ 96 ], the corn stalk borer S. nonagrioides [ 70 ], the wheat midge S. mosellana [ 97 ], the butterfly Pieris melete [ 98 ], the leaf beetle Gastrophysa atrocyanea [ 99 ] and the silkworm B. mori [ 100 ]. The augmentation of sHSPs appears to be common during diapause in numerous species across different insect orders, including Lepidoptera, Diptera, Hymenoptera and Coleoptera, and can occur at different developmental stages (embryo, larva, pupa, adult) [ 72 ].…”

Section: Discussionmentioning

confidence: 99%

Prolonged Heat Stress during Winter Diapause Alters the Expression of Stress-Response Genes in Ostrinia nubilalis (Hbn.)

Uzelac,

Avramov,

Knežić

et al. 2024

IJMS

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During diapause, a state of temporarily arrested development, insects require low winter temperatures to suppress their metabolism, conserve energy stores and acquire cold hardiness. A warmer winter could, thus, reduce diapause incidence and duration in many species, prematurely deplete their energy reserves and compromise post-diapause fitness. In this study, we investigated the combined effects of thermal stress and the diapause program on the expression of selected genes involved in antioxidant defense and heat shock response in the European corn borer Ostrinia nubilalis. By using qRT-PCR, it has been shown that response to chronic heat stress is characterized by raised mRNA levels of grx and trx, two important genes of the antioxidant defense system, as well as of hsp70 and, somewhat, of hsp90, two major heat shock response proteins. On the other hand, the expression of hsc70, hsp20.4 and hsp20.1 was discontinuous in the latter part of diapause, or was strongly controlled by the diapause program and refractory to heat stress, as was the case for mtn and fer, genes encoding two metal storage proteins crucial for metal ion homeostasis. This is the first time that the effects of high winter temperatures have been assessed on cold-hardy diapausing larvae and pupae of this important corn pest.

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Section: Discussionmentioning

confidence: 99%

Prolonged Heat Stress during Winter Diapause Alters the Expression of Stress-Response Genes in Ostrinia nubilalis (Hbn.)

Uzelac,

Avramov,

Knežić

et al. 2024

IJMS

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show abstract

“…HSPs were first identified in the insect Drosophila melanogaster by Ritossa et al [2]. They occur when organisms are subjected to stress, including starvation, extreme temperature, virus invasion, heavy metals, chemical agents, and UV light [3][4][5][6]. HSPs act as molecular chaperones that can protect proteins from irreversible denaturation and help proteins to refold correctly [7,8].…”

Section: Introductionmentioning

confidence: 99%

Spatiotemporal Patterns of Five Small Heat Shock Protein Genes in Hyphantria cunea in Response to Thermal Stress

Zhao,

Liu,

et al. 2023

IJMS

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Hyphantria cunea (Drury), a destructive polyphagous pest, has been spreading southward after invading northern China, which indicates that this insect species is facing a huge thermal challenge. Small heat shock proteins (sHSPs) function as ATP-independent molecular chaperones that protect insects from heat stress damage. In order to explore the role of sHSPs in the thermotolerance of H. cunea, five novel sHSP genes of H. cunea were cloned, including an orthologous gene (HcHSP21.4) and four species-specific sHSP genes (HcHSP18.9, HcHSP20.1, HcHSP21.5, and HcHSP29.8). Bioinformatics analysis showed that the proteins encoded by these five HcHSPs contained typical α-crystallin domains. Quantitative real-time PCR analysis revealed the ubiquitous expression of all HcHSPs across all developmental stages of H. cunea, with the highest expression levels in pupae and adults. Four species-specific HcHSPs were sensitive to high temperatures. The expression levels of HcHSPs were significantly up-regulated under heat stress and increased with increasing temperature. The expression levels of HcHSPs in eggs exhibited an initial up-regulation in response to a temperature of 40 °C. In other developmental stages, the transcription of HcHSPs was immediately up-regulated at 30 °C or 35 °C. HcHSPs transcripts were abundant in the cuticle before and after heat shock. The expression of HcHSP21.4 showed weak responses to heat stress and constitutive expression in the tissues tested. These results suggest that most of the HcHSPs are involved in high-temperature response and may also have functions in the normal development and reproduction of H. cunea.

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Molecular characterization and expression analysis of six small heat shock protein genes in Trogoderma granarium during cold and starvation-induced larval diapause

Dageri

2024

Journal of Stored Products Research

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Identification and up-regulation of three small heat shock proteins in summer and winter diapause in response to temperature stress in Pieris melete

Cited by 3 publications

References 48 publications

Prolonged Heat Stress during Winter Diapause Alters the Expression of Stress-Response Genes in Ostrinia nubilalis (Hbn.)

Prolonged Heat Stress during Winter Diapause Alters the Expression of Stress-Response Genes in Ostrinia nubilalis (Hbn.)

Spatiotemporal Patterns of Five Small Heat Shock Protein Genes in Hyphantria cunea in Response to Thermal Stress

Molecular characterization and expression analysis of six small heat shock protein genes in Trogoderma granarium during cold and starvation-induced larval diapause

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