Identification and typing of free-living  Acanthamoeba  spp. by MALDI-TOF MS Biotyper

Chierico, Federica Del; Cave, David Di; Accardi, Cristel; Santoro, Maristella; Masotti, Andrea; D’Alfonso, Rossella; Berrilli, Federica; Urbani, Andrea; Putignani, Lorenza

doi:10.1016/j.exppara.2016.09.007

“…The proteomic methodology has been used for the identification of many agents of infectious diseases, such as bacteria [ 24 , 25 ], protozoa [ 26 , 27 ] and fungi [ 28 – 31 ]. Some proteomic studies have been performed to identify Paracoccidioides spp.…”

Section: Discussionmentioning

confidence: 99%

Ceruloplasmin, transferrin and apolipoprotein A-II play important role in treatment's follow-up of paracoccidioidomycosis patients

Sylvestre

¹

,

Cavalcante

²

,

Silva

³

et al. 2018

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Paracoccidioidomycosis (PCM) is a systemic disease caused by thermodymorphic fungi of the Paracoccidioides brasiliensis complex, (Paracoccidioides spp.). Patients with PCM reveal specific cellular immune impairment. Despite the effective treatment, quiescent fungi can lead to relapse, usually late, the serological diagnosis of which has been deficient. The present study was carried out with the objective of investigating a biomarker for the identification of PCM relapse and another molecule behaving as an immunological recovery biomarker; therefore, it may be used as a cure criterion. In the evolutionary analysis of the proteins identified in PCM patients, comparing those that presented with those that did not reveal relapse, 29 proteins were identified. The interactions observed between the proteins, using transferrin and haptoglobin, as the main binding protein, were strong with all the others. Patient follow-up suggests that cerulosplamin may be a marker of relapse and that transferrin and apolipoprotein A-II may contribute to the evaluation of the treatment efficacy and avoiding a premature decision.

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“…The proteomic methodology has been used for the identification of many agents of infectious diseases, such as bacteria [ 17 , 18 ], protozoa [ 19 , 20 ] and fungi [ 21 – 24 ]. Some proteomic studies have been performed to identify Paracoccidioides spp.…”

Section: Discussionmentioning

confidence: 99%

Serological proteomic biomarkers to identify Paracoccidioides species and risk of relapse

Sylvestre

¹

,

Cavalcante

²

,

Silva

³

et al. 2018

View full text Add to dashboard Cite

The sensitivity of the double agar gel immunodiffusion test is about 90% in patients with untreated paracoccidioidomycosis (PCM), but it is much lower in cases of relapse. In addition, serum from patients with PCM caused by Paracoccidioides lutzii, frequent in the Midwest region of Brazil, do not react with the classical antigen obtained from Pb B-339. These findings showed the need for alternative diagnostic methods, such as biological markers through proteomics. The aim of this study was to identify biomarkers for the safe identification of PCM relapse and specific proteins that could distinguish infections caused by Paracoccidioides brasiliensis from those produced by Paracoccidioides lutzii. Proteomic analysis was performed in serum from 9 patients with PCM caused by P. brasiliensis, with and without relapse, from 4 patients with PCM produced by P. lutzii, and from 3 healthy controls. The comparative evaluation of the 29 identified plasma proteins suggested that the presence of the immunoglobulin (Ig) alpha-2 chain C region and the absence of Ig heavy chain V-III TIL indicate infection by P. lutzii. In addition, the absence of complement factor B protein might be a predictor of relapse. The evaluation of these proteins in a higher number of patients should be carried out in order to validate these findings.

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“…The proteomic methodology has been used for the identification of many agents of infectious diseases, such as bacteria [17,18], protozoa [19,20] and fungi [21][22][23][24]. Some proteomic studies have been performed to identify Paracoccidioides spp.…”

Section: Discussionmentioning

confidence: 99%

Serological proteomic biomarkers to identify Paracoccidioides species and risk of relapse.

Sylvestre

¹

,

Cavalcante

²

,

Silva

³

et al. 2018

Preprint

0

View full text Add to dashboard Cite

The sensitivity of the double agar gel immunodiffusion test is about 90% in patients with untreated paracoccidioidomycosis (PCM), but it is much lower in cases of relapse. In addition, serum from patients with PCM caused by Paracoccidioides lutzii, frequent in the Midwest region of Brazil, do not react with the classical antigen obtained from Pb B-339. These findings showed the need for alternative diagnostic methods, such as biological markers through proteomics. The aim of this study was to identify biomarkers for the safe identification of PCM relapse and specific proteins that could distinguish infections caused by Paracoccidioides brasiliensis from those produced by Paracoccidioides lutzii. Proteomic analysis was performed in serum from 9 patients with PCM caused by P. brasiliensis, with and without relapse, from 4 patients with PCM produced by P. lutzii, and from 3 healthy controls. The comparative evaluation of the 29 identified plasma proteins suggested that the presence of the immunoglobulin (Ig) alpha-2 chain C region and the absence of Ig heavy chain V-III TIL indicate infection by P. lutzii. In addition, the absence of complement factor B protein might be a predictor of relapse. The evaluation of these proteins in a higher number of patients should be carried out in order to validate these findings.

show abstract

Identification and typing of free-living Acanthamoeba spp. by MALDI-TOF MS Biotyper

Cited by 15 publications

References 38 publications

Ceruloplasmin, transferrin and apolipoprotein A-II play important role in treatment's follow-up of paracoccidioidomycosis patients

Ceruloplasmin, transferrin and apolipoprotein A-II play important role in treatment's follow-up of paracoccidioidomycosis patients

Serological proteomic biomarkers to identify Paracoccidioides species and risk of relapse

Serological proteomic biomarkers to identify Paracoccidioides species and risk of relapse.

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