Identification and transcript analysis of two glutamate decarboxylase genes, CsGAD1 and CsGAD2, reveal the strong relationship between CsGAD1 and citrate utilization in citrus fruit

Liu, Xiao; Hu, Xuehai; Jin, Long-Fei; Shi, Cai-Yun; Liu, Yong-Zhong; Peng, Shuang

doi:10.1007/s11033-014-3506-x

Cited by 51 publications

(44 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…qRT-PCR was performed in a 10 μL reaction volume that contained 5 μL Thunderbird TM SYBR qPCR Mix (TOYOBO, JAPAN), 1 μL of cDNA, 1 μM gene-specific primers and 3 μL ddH 2 O. Reactions started with an initial incubation at 50°C for 2 min and 95°C for 10 min and were then subjected to 40 cycles of 95°C for 15 s and 60°C for 60 s (Liu et al, 2014). The Livak (Livak and Schmittgen, 2001) method was employed to calculate gene relative expression levels.…”

Section: Methodsmentioning

confidence: 99%

Transcriptome Changes Associated with Boron Deficiency in Leaves of Two Citrus Scion-Rootstock Combinations

et al. 2017

Self Cite

View full text Add to dashboard Cite

Boron (B) deficiency stress is frequently observed in citrus orchards and causes considerable loss of productivity and fruit quality. Carrizo citrange (Cc) has been reported as a rootstock more tolerant to B deficiency than Trifoliate orange (To). The ‘Newhall’ navel orange (Ns) performed better when grafted onto Cc (Ns/Cc) than when grafted onto To (Ns/To) under long-term B deficiency. The present study confirmed that Ns/Cc had higher boron content, leaf fresh weight, lower leaf chlorosis and stronger photosynthesis ability than Ns/To. Moreover, B-deficiency significantly reduced the chlorophyll and carotenoid content in Ns/To. The content of total soluble sugar and lignin were dramatically increased and the expression levels of photosynthesis-related genes were substantially down-regulated in Ns/To by B-deficient treatment. B-deficiency also strongly induced expression levels of chlorophyll decomposition-related genes, glucose synthesis-related genes and lignin synthesis-related genes, and significantly inhibited the expression of carotenoid synthesis-related genes in Ns/To. Overall, these findings suggested that the influence of To on the scion of Ns was worse than that of Cc due to differently regulating these metabolic pathways under the long term of B-deficiency. The transcriptome analysis provided further information for understanding the mechanism of the different responses of scion-rootstock combinations to B-deficiency stress.

show abstract

Section: Methodsmentioning

confidence: 99%

Transcriptome Changes Associated with Boron Deficiency in Leaves of Two Citrus Scion-Rootstock Combinations

et al. 2017

Self Cite

View full text Add to dashboard Cite

show abstract

“…Each pair of fruits was grown on the identical fruit-bearing shoot, and the fruits were similar in size. The ABA injection was performed according to Liu et al (2014). In each pair of fruits, one fruit was injected with an ABA solution, and one fruit, the control, was injected only with the solvent (without the ABA).…”

Section: Aba Treatmentmentioning

confidence: 99%

Citrus sucrose transporter genes: genome-wide identification and transcript analysis in ripening and ABA-injected fruits

Islam

Jin

Shi

et al. 2015

Tree Genetics & Genomes

Self Cite

View full text Add to dashboard Cite

The sucrose transporter (SUT) plays a major role in the transport of sucrose apoplastically. An extensive mining of the data confirmed that at least three SUT genes (CitSUT1, CitSUT2, and CitSUT3) were found in the databases of the three currently available citrus genomes. The exon and intron sizes varied among the three CitSUT genes, and the similarities among the genes were 40-50 %. The phylogenetic analysis revealed that the CitSUT1, CitSUT2, and CitSUT3 were distributed into groups SUT I, SUT II, and SUT III, respectively. The analysis of the spatiotemporal expression of the genes showed that the CitSUT1, CitSUT2, and CitSUT3 were predominantly expressed in the stamens, young leaves, and fruits [juice sacs (JS) and/or segment membrane (SM)], respectively. Moreover, in addition to the significant increase in sucrose levels from 114 days after anthesis (DAA) to 160 DAA, the transcript levels of CitSUT1 and CitSUT3 increased significantly in the JS and decreased significantly in the SM, whereas the CitSUT2 transcript levels decreased significantly either in the JS or in the SM. Additionally, treatment with ABA increased the accumulations of sucrose and fructose in fruits, which were accompanied by the induction of the CitSUT2 transcripts in fruits (JS and SM) and the CitSUT3 transcripts in the SM and the reduction of the CitSUT1 and CitSUT3 transcript levels in the JS. Thus, the CitSUT1 and CitSUT3 might play important roles in the transport of sucrose into the fruit JS during normal fruit development; the transcript alterations of the CitSUT2 and CitSUT3 under the ABA treatment might contribute to the increased accumulation of sucrose.

show abstract

“…SHM7 , also known as AT1G36370, encodes the specific serine hydroxy methyltransferase 7, which further contributes to catalyzing the interconversion of serine and glycine [71]. Recent publications confirm that the main function of SHM7, pyridoxal phosphate binding, may contribute to the development and maturation of fruit in citrus, implying its potential role in various plant subtypes [72, 73]. Furthermore, a specific mutant screening of Arabidopsis thaliana , based on high-throughput HPLC-MS/MS assay has confirmed that, together with another enzyme threonine aldolase, the serine hydroxy methyltransferase may play a specific role during the development and maturation of fruit, affecting seed nutritional quality [74].…”

Section: Discussionmentioning

confidence: 99%

Identifying novel fruit-related genes in Arabidopsis thaliana based on the random walk with restart algorithm

Zhang¹,

Dai²,

Liu³

et al. 2017

PLoS ONE

View full text Add to dashboard Cite

Fruit is essential for plant reproduction and is responsible for protection and dispersal of seeds. The development and maturation of fruit is tightly regulated by numerous genetic factors that respond to environmental and internal stimulation. In this study, we attempted to identify novel fruit-related genes in a model organism, Arabidopsis thaliana, using a computational method. Based on validated fruit-related genes, the random walk with restart (RWR) algorithm was applied on a protein-protein interaction (PPI) network using these genes as seeds. The identified genes with high probabilities were filtered by the permutation test and linkage tests. In the permutation test, the genes that were selected due to the structure of the PPI network were discarded. In the linkage tests, the importance of each candidate gene was measured from two aspects: (1) its functional associations with validated genes and (2) its similarity with validated genes on gene ontology (GO) terms and KEGG pathways. Finally, 255 inferred genes were obtained, subsequent extensive analysis of important genes revealed that they mainly contribute to ubiquitination (UBQ9, UBQ8, UBQ11, UBQ10), serine hydroxymethyl transfer (SHM7, SHM5, SHM6) or glycol-metabolism (HXKL2_ARATH, CSY5, GAPCP1), suggesting essential roles during the development and maturation of fruit in Arabidopsis thaliana.

show abstract

Identification and transcript analysis of two glutamate decarboxylase genes, CsGAD1 and CsGAD2, reveal the strong relationship between CsGAD1 and citrate utilization in citrus fruit

Cited by 51 publications

References 55 publications

Transcriptome Changes Associated with Boron Deficiency in Leaves of Two Citrus Scion-Rootstock Combinations

Transcriptome Changes Associated with Boron Deficiency in Leaves of Two Citrus Scion-Rootstock Combinations

Citrus sucrose transporter genes: genome-wide identification and transcript analysis in ripening and ABA-injected fruits

Identifying novel fruit-related genes in Arabidopsis thaliana based on the random walk with restart algorithm

Contact Info

Product

Resources

About