Identification and transcript analysis of MATE genes involved in anthocyanin transport in radish ( Raphanus sativus  L.)

M’mbone, Muleke Everlyne; Cheng, Wanwan; Xu, Liang; Wang, Yan; Karanja, Bernard Kinuthia; Zhu, Xiaoming; Cao, Yang; Liu, Liwang

doi:10.1016/j.scienta.2018.04.029

Cited by 26 publications

(21 citation statements)

References 29 publications

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“…Recent research indicated that multi-drug and toxic extrusion MATE TT12 might be involved in transportation of anthocyanin across cell membranes in brown cotton, radish and strawberry (Gao et al, 2016, M’Mbone et al, 2018, Chen et al, 2018). In the present study, six MATE TT12 genes were identified in the strawberries and high ambient temperature up-regulate their genes expression, which was consistant with the increment of anthocyanidins levels (Table 1).…”

Section: Discussionmentioning

confidence: 99%

Comparative transcriptome analysis reveals fruit discoloration mechanisms in postharvest strawberries in response to high ambient temperature

et al. 2019

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Highlight High ambient temperature induced fruit discoloration of postharvest strawberries. High ambient temperature enhanced anthocyanin biosynthesis and accumulation. UAGT was the crucial enzymes involving anthocyanin glucosides in postharvest strawberries. High ambient temperature stimulated genes expression of laccase and peroxidase. Anthocyanin synthesis and degradation co-existed in postharvest strawberries under high ambient temperature.

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Section: Discussionmentioning

confidence: 99%

Comparative transcriptome analysis reveals fruit discoloration mechanisms in postharvest strawberries in response to high ambient temperature

et al. 2019

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“…In other anthocyanin-producing species, two other mechanisms of vacuolar localization have been identified in addition to the GST/ABC transporter system—MATE transporters and vesicle trafficking. MATE transporters, either as H + /flavonoid antiporters or in combination with other vacuolar proton gradient maintaining mechanisms, have been identified in Arabidopsis, Medicago , tomato (S. lycopersicum ), and radish ( R. sativa ) ( Debeaujon et al 2001 ; Mathews et al 2003 ; Gomez et al 2009 ; Zhao et al 2011 ; Kitamura et al 2016 ; M’mbone et al 2018 ). Support for vesicle trafficking of anthocyanins and a subsequent autophagy-like mechanism of vacuolar transport has been found in Arabidopsis, Brassica napus , and grapevine ( V. vinifera) .…”

Section: Introductionmentioning

confidence: 99%

Linking anthocyanin diversity, hue, and genetics in purple corn

Chatham

Juvik

2021

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While maize with anthocyanin-rich pericarp (purple corn) is rising in popularity as a source of natural colorant for foods and beverages, information on color range and stability—factors associated with anthocyanin decorations and compositional profiles—is currently limited. Furthermore, to maximize the scalability and meet growing demands, both anthocyanin concentrations and agronomic performance must improve in purple corn varieties. Using the natural anthocyanin diversity present in a purple corn landrace, Apache Red, we generated a population with variable flavonoid profiles—flavanol–anthocyanin condensed forms (0–83%), acylated anthocyanins (2–72%), pelargonidin-derived anthocyanins (5–99%), C-glycosyl flavone co-pigments up to 1904 µg/g, and with anthocyanin content up to 1598 µg/g. Each aspect of the flavonoid profiles was found to play a role in either the resulting extract hue or intensity. With genotyping-by-sequencing of this population, we mapped aspects of the flavonoid profile. Major quantitative trait loci (QTLs) for anthocyanin type were found near loci previously identified only in aleurone-pigmented maize varieties [Purple aleurone1 (Pr1) and Anthocyanin acyltransferase1 (Aat1)]. A QTL near P1 (Pericarp color1) was found for both flavone content and flavanol–anthocyanin condensed forms. A significant QTL associated with peonidin-derived anthocyanins near a candidate S-adenosylmethionine-dependent methyltransferase was also identified, warranting further investigation. Mapping total anthocyanin content produced signals near Aat1, the aleurone-associated bHLH R1 (Colored1), the plant color-associated MYB, Pl1 (Purple plant1), the aleurone-associated recessive intensifier, In1 (Intensifier1), and several previously unidentified candidates. This population represents one of the most anthocyanin diverse pericarp-pigmented maize varieties characterized to date. Moreover, the candidates identified here will serve as branching points for future research studying the genetic and molecular processes determining anthocyanin profile in pericarp.

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“…Several other significant SNPs or SNP ranges were observed ( Table 2). On Chr 7, a peak was found at 156.4 Mb, which aligns closely with a potential ortholog of the Arabidopsis TT12 (Transparent Testa12) MATE transporter of flavonoids (Zm00001d021629, 158.6 Mb) 12 and other flavonoid transporting MATEs in other species ( Supplementary Figure 3) 14,15,17 . This is particularly interesting given the highly significant effect of SNPs near Aat1 for both competitive flux and overall flux (Figures 4 and 5).…”

Section: Mapping Molar Fluxmentioning

confidence: 57%

“…In other anthocyanin-producing species two other mechanisms of vacuolar localization have been identified in addition to the GST/ABC transporter system -MATE-transporters and vesicle trafficking. MATE-transporters, either as H + /flavonoid antiporters or in combination with other vacuolar proton gradient maintaining mechanisms have been identified in Arabidopsis, Medicago, tomato (S. lycopersicum) and radish (R. sativa) [12][13][14][15][16][17] . Support for vesicle trafficking of anthocyanins and a subsequent autophagy-like mechanism of vacuolar transport has been found in Arabidopsis, Brassica napus, and grapevine (V. vinifera).…”

Section: Introductionmentioning

confidence: 99%

Gwas and Genomic Selection For Increased Anthocyanin Content in Purple Corn

Juvik

Chatham

2020

Preprint

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Purple corn offers an attractive source of economical natural anthocyanin-based colorant for use in foods and beverages. Yet to maximize the scalability and meet growing demands, both anthocyanin concentrations and agronomic performance must improve in purple corn varieties.We studied flux through the flavonoid biosynthesis pathway using GWAS data derived from a diverse purple corn landrace with anthocyanin-rich pericarp, Apache Red. Trends between flavonoid endpoints suggest that regulators of total flux into the pathway and regulators of partitioning within the pathway may both represent targets for maximizing anthocyanin content.A peak at the end of chromosome 1 near Aat1 (Anthocyanin acyltransferase1) was highly significant in all approaches taken to map anthocyanin flux, suggesting the structural modification of malonylation is required for maximal anthocyanin production. We also identified several candidate MATEs and H + ATPases that could assist in the preferential transport of acylated anthocyanins into the vacuole. These and other candidates identified here suggest there is still much to learn about the regulation of anthocyanin biosynthesis in the pericarp of purple corn. The efficacy of genomic predictions in the population was also studied, yielding an accuracy of 0.71 with cross validation for total anthocyanin content with no improvement found when known anthocyanin regulators were added to the model. These data suggest that genomic selection could be employed effectively in a purple corn breeding program, and especially for a landrace improvement program.

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Identification and transcript analysis of MATE genes involved in anthocyanin transport in radish ( Raphanus sativus L.)

Cited by 26 publications

References 29 publications

Comparative transcriptome analysis reveals fruit discoloration mechanisms in postharvest strawberries in response to high ambient temperature

Comparative transcriptome analysis reveals fruit discoloration mechanisms in postharvest strawberries in response to high ambient temperature

Linking anthocyanin diversity, hue, and genetics in purple corn

Gwas and Genomic Selection For Increased Anthocyanin Content in Purple Corn

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