Identification and tracing ofEnterococcusspp. by RAPD-PCR in traditional fermented sausages and meat environment

Abstract

Aims: Four local small‐scale factories were studied to determine the sources of enterococci in traditional fermented sausages. Methods and Results: Different points during the production of a traditional fermented sausage type (fuet) were evaluated. Randomly amplified polymorphic DNA (RAPD)‐PCR was used to type 596 Enterococcus isolates from the final products, the initial meat batter, the casing, the workers’ hands and the equipment. Species‐specific PCR‐multiplex and the partial sequencing of atpA gene and 16S rRNA gene sequencing allowed the identification of the isolates: Enterococcus faecalis (31·4%), Enterococcus faecium (30·7%), Enterococcus sanguinicola (14·9%), Enterococcus devriesei (9·7%), Enterococcus malodoratus (7·2%), Enterococcus gilvus (1·0%), Enterococcus gallinarum (1·3%), Enterococcus casseliflavus (3·4%), Enterococcus hermanniensis (0·2%), and Enterococcus durans (0·2%). A total of 92 different RAPD‐PCR profiles were distributed among the different factories and samples evaluated. Most of the genotypes found in fuet samples were traced back to their source. Conclusions: The major sources of enterococci in the traditional fermented sausages studied were mainly the equipment followed by the raw ingredients, although a low proportion was traced back to human origin. Significance and Impact of the Study: This work contributes to determine the source of enterococcal contamination in fermented sausages and also to the knowledge of the meat environment.

Role of House Flies in the Ecology of Enterococcus faecalis from Wastewater Treatment Facilities

“…faecium at WWTF-1 was 9:1, which is similar to other studies that have surveyed enterococci at meat processing operations (beef, pork and poultry) and from associated meat products. In most of those studies, E. faecalis was dominant followed by E. faecium [14,30,36,45,49,54,58]. In the current study, the species composition among sludge and house flies was comparable with E. faecalis being the most abundant species from both sources (Fig.…”

Köpek ve Kedi Rektal Svablarından İzole Edilen Ampisilin-Dirençli Enterokokların İncelenmesi

“…The suspension was centrifuged at 12000 g for 1.5 min and 100 µl of the supernatant was transferred to a new tube and used as a template DNA (Martin et al, 2009).…”

Caracterización y potencial probiótico de bacterias lácticas aisladas de leche de oveja guirra

“…Estudios previos destacan la técnica de difusión en placa por el método de sobrenadante porque se observan halos de inhibición más claros y puede deberse a que es una técnica sencilla y es menos susceptible a contaminaciones (Hütt et al, 2005;Sigrid et al, 2006 (Armuzzi et al, 2001). Al igual que L. brevis, estas cepas producen sustancias antimicrobianas que inhiben la multiplicación de distintos enteropatógenos (Salminen y Donohhue, 1996;Isolauri et al, 2002;Warren et al, 2007;Calderón et al, 2007;B. Martín, et al, 2009).…”