Identification and the developmental formation of carotenoid pigments in the yellow/orange Bacillus spore-formers

Pérez-Fons, Laura; Steiger, Sabine; Khaneja, R.; Bramley, Peter M.; Cutting, Simon M.; Sandmann, Gerhard; Fraser, Paul D.

doi:10.1016/j.bbalip.2010.12.009

Cited by 56 publications

(66 citation statements)

References 40 publications

(62 reference statements)

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“…Both sub-clusters are transcribed in opposite directions. In B. indicus, the carotenoid product 4-glycosyl-49-methyl-4,49-diapolycopene-49-oate fatty acid ester (Perez-Fons et al, 2011) requires the catalytic activity of a glycosyltransferase (GT) with the terminal hydroxyl group acting as the sugar acceptor (Lairson et al, 2008). A corresponding AT gene is present next to crtNb.…”

Section: Resultsmentioning

confidence: 99%

“…A C 30 carotenoid biosynthesis pathway has been established for two Bacillus species. In Bacillus indicus, the major carotenoid is methyl 49-[6-O-acylglycosyl)oxy]-4,49-diapolycopen-4-oic acid (Perez-Fons et al, 2011), whereas in Bacillus firmus, 4,49-diapolycopen-4,49-dioic acid with putative glycosyl esters accumulate (Steiger et al, 2012b;Osawa et al, 2013), all sharing 4,49-diapolycopen-4-oic acid (4,49-diapo-y,y-caroten-4-oic acid) as a precursor. In some pigmented bacilli, formation of the end products of the carotenoid pathway is associated with spore formation (Duc et al, 2006;Perez-Fons et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

“…In Bacillus indicus, the major carotenoid is methyl 49-[6-O-acylglycosyl)oxy]-4,49-diapolycopen-4-oic acid (Perez-Fons et al, 2011), whereas in Bacillus firmus, 4,49-diapolycopen-4,49-dioic acid with putative glycosyl esters accumulate (Steiger et al, 2012b;Osawa et al, 2013), all sharing 4,49-diapolycopen-4-oic acid (4,49-diapo-y,y-caroten-4-oic acid) as a precursor. In some pigmented bacilli, formation of the end products of the carotenoid pathway is associated with spore formation (Duc et al, 2006;Perez-Fons et al, 2011). In contrast to S. aureus, in which the genes for the 4,49-diaponeurosporene-derived biosynthesis of staphyloxanthin were identified and functionally assigned (Pelz et al, 2005;Kim & Lee, 2012), the genes for the oxidative C 30 pathway via 4,49-diapolycopene are unknown.…”

Section: Introductionmentioning

confidence: 99%

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Annotation and functional assignment of the genes for the C30 carotenoid pathways from the genomes of two bacteria: Bacillus indicus and Bacillus firmus

et al. 2015

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Bacillus indicus and Bacillus firmus synthesize C 30 carotenoids via farnesyl pyrophosphate, forming apophytoene as the first committed step in the pathway. The products of the pathways were methyl 49-[6-O-acyl-glycosyl)oxy]-4,49-diapolycopen-4-oic acid and 4,49-diapolycopen-4,49-dioic acid with putative glycosyl esters. The genomes of both bacteria were sequenced, and the genes for their early terpenoid and specific carotenoid pathways annotated. All genes for a functional 1-deoxy-D-xylulose 5-phosphate synthase pathway were identified in both species, whereas genes of the mevalonate pathway were absent. The genes for specific carotenoid synthesis and conversion were found on gene clusters which were organized differently in the two species. The genes involved in the formation of the carotenoid cores were assigned by functional complementation in Escherichia coli. This bacterium was co-transformed with a plasmid mediating the formation of the putative substrate and a second plasmid with the gene of interest. Carotenoid products in the transformants were determined by HPLC. Using this approach, we identified the genes for a 4,49-diapophytoene synthase (crtM), 4,49-diapophytoene desaturase (crtNa), 4,49-diapolycopene ketolase (crtNb) and 4,49-diapolycopene aldehyde oxidase (crtNc). The three crtN genes were closely related and belonged to the crtI gene family with a similar reaction mechanism of their enzyme products. Additional genes encoding glycosyltransferases and acyltransferases for the modification of the carotenoid skeleton of the diapolycopenoic acids were identified by comparison with the corresponding genes from other bacteria.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Annotation and functional assignment of the genes for the C30 carotenoid pathways from the genomes of two bacteria: Bacillus indicus and Bacillus firmus

et al. 2015

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“…Recently, scientists at the Royal Holloway University of London have isolated carotenoid-producing spore-forming Bacillus spp.. These pigmented Bacillus species (yellow, pink, orange…) have been characterized and the pigments have been shown to be due to one or more carotenoids (Duc et al, 2006;Hong et al, 2009;Khaneja et al, 2010;Perez-Fons et al, 2011). More specifically, the presence of 1-HO-demethylspheroidene, ubiquinone and phytoene was identified in some strains by means of a combination of HPLC analysis and UV/VIS spectral data.…”

Section: Bacteria As Production Facilitiesmentioning

confidence: 99%

The Gut Microbiota as Target for Innovative Drug Development: Perspectives and a Case Study of Inflammatory Bowel Diseases

Vermeiren¹,

Possemiers²,

Marzorati³

et al. 2011

Drug Development - A Case Study Based Insight Into Modern Strategies

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“…Chromatographic procedures followed that of Perez-Fons et al (2011). Briefly, separations were made on an RP C 30 3 μm column (150 × 2.1 mm i.d.…”

Section: Lc-ms For Mutatochrome (β-Carotene 58-epoxide) Identificationmentioning

confidence: 99%

Carotenoid profiling of yams: Clarity, comparisons and diversity

et al. 2018

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A B S T R A C TScreening carotenoids of elite accessions of yam (Dioscorea spp.) used in the global yam breeding program has been conducted to quantitatively determine the carotenoid composition of the crop. Comparisons to previous data reporting cerotenoid levels in yam has been made, in order to deduce greater perspectives across multiple studies. Characterisation of complex species and accession -specific profiles have shown a rich base of diversity that can inform breeding strategies. Key findings include; (i) the identification of accessions rich in β-carotene which can aid provitamin A biofortification, (ii) Data disputing the commonly held belief that yellow Guinea yam (D. cayennensis) has higher β-carotene content than that of white Guinea yam (D. rotundata), and (iii) the tentative identification of C 25 -epoxy-apocarotenoid persicaxanthin with potential implications for tuber dormancy.

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Identification and the developmental formation of carotenoid pigments in the yellow/orange Bacillus spore-formers

Cited by 56 publications

References 40 publications

Annotation and functional assignment of the genes for the C30 carotenoid pathways from the genomes of two bacteria: Bacillus indicus and Bacillus firmus

Annotation and functional assignment of the genes for the C30 carotenoid pathways from the genomes of two bacteria: Bacillus indicus and Bacillus firmus

The Gut Microbiota as Target for Innovative Drug Development: Perspectives and a Case Study of Inflammatory Bowel Diseases

Carotenoid profiling of yams: Clarity, comparisons and diversity

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