Identification and synthesis of an antigenic determinant common to human chorionic somatomammotropin and human growth hormone

An epitope of human chorionic somatomammotropin for one of the monoclonal antibodies raised against the whole antigen has been identified. We compared the release of peptides from limited proteolysis of the antigen in the presence and absence of the related antibody. Using enzymes of different specificity, we could determine the amino acid sequence that can be considered at least inclusive of the epitope. The monoclonal antibody selected is 100% cross-reactive with human growth hormone, so the antigenic determinant identified is shared by the two protein hormones.

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An antigenic determinant common to human chorionic somatomammotropin and human growth hormone revealed by limited proteolysis of immune complexes

Santucci

Rustici

Bracci

et al. 1991

Biopolymers

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Two new Protecting Groups for the Guanidino Function of arginine

et al. 1995

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Two new synthons, Fmoc‐L‐Arg(biphenyl‐4‐sulphonyl)‐OH (8) and Fmoc‐Arg(4‐methoxy‐3‐t‐butylbenzenesulphonyl)‐OH (14), are prepared for the synthesis of arginine‐containing peptides. These groups are cleaved by commonly employed trifluoroacetic acid and methanesulphonic acid. Kinetic studies reveal that extended bicyclic aromatic conjugation, as in biphenyl, slightly improves the acid lability compared to the electron‐donating t‐butyl group.

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Blockade of the antigen-antibody reaction using benzil condensation with the guanidyl residue of arginine

1991

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Benzil blockade of the guanidyl group of arginine was tried on sections of paraffin-embedded tissue fixed in two different fixatives, in an attempt to evaluate the relevance of this amino acid to the reaction of several proteins with their corresponding antibodies. The two fixatives were 10% formaldehyde, and Bouin's fluid without acetic acid. Both polyclonal and monoclonal antibodies against proteins or peptides (lysozyme, adrenocorticotropic hormone, growth hormone, placental lactogen, and prolactin) were used on human biopsies or material from autopsies. The blockade was effective when monoclonal antibodies were used, whereas no effect or only a small decrease of the intensity of the reaction was observed with polyclonal antibodies. The least definitive result was obtained with prolactin, where a complete blockade was never achieved with monoclonal antibodies. Calcitonin, a peptide that does not contain arginine, was used as a control not susceptible to benzil blockade; no blockade of immunostaining was observed.

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Identification and synthesis of an antigenic determinant common to human chorionic somatomammotropin and human growth hormone

Cited by 16 publications

References 26 publications

An antigenic determinant common to human chorionic somatomammotropin and human growth hormone revealed by limited proteolysis of immune complexes

An antigenic determinant common to human chorionic somatomammotropin and human growth hormone revealed by limited proteolysis of immune complexes

Two new Protecting Groups for the Guanidino Function of arginine

Blockade of the antigen-antibody reaction using benzil condensation with the guanidyl residue of arginine

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