Identification and sex-biased profiles of candidate olfactory genes in the antennal transcriptome of the parasitoid wasp Cotesia vestalis

Liu, Yipeng; Du, Lixiao; Zhu, Yun; Yang, Shiyong; Zhou, Qiong; Wang, Guirong; Liu, Yang

doi:10.1016/j.cbd.2020.100657

Cited by 22 publications

(20 citation statements)

References 79 publications

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“…Among these gene families, OBPs are a class of small soluble proteins widely present in the lymph fluid of the antennal sensilla, which can bind and transfer such molecules to the receptors [ 7 ], and participate in host location and pheromone perception [ 8 , 9 ]. The combination of OBPs and volatile molecules is the foremost step for insects to recognize odorous pheromones [ 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

Antennal Transcriptome Analysis and Identification of Olfactory Genes in Glenea cantor Fabricius (Cerambycidae: Lamiinae)

Ouyang

et al. 2022

Insects

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Glenea cantor Fabricius (Cerambycidae: Lamiinae) is a pest that devastates urban landscapes and causes ecological loss in southern China and Southeast Asian countries where its main host kapok trees are planted. The olfactory system plays a vital role in mating, foraging, and spawning in G. cantor as an ideal target for pest control. However, the olfactory mechanism of G. cantor is poorly understood at the molecular level. In this study, we first established the antennal transcriptome of G. cantor and identified 76 olfactory-related genes, including 29 odorant binding proteins (OBPs), 14 chemosensory proteins (CSPs), 13 odorant receptors (ORs), 18 ionotropic receptors (IRs) and 2 sensory neuron membrane proteins (SNMPs). Furthermore, the phylogenetic trees of olfactory genes were constructed to study the homology with other species of insects. We also verified the reliability of transcriptome differential genes by qRT-PCR, which indicated the reliability of the transcriptome. Based on the relative expression of 30 d adults, GcanOBP22 and GcanOBP25 were highly expressed not only in the antennae, but also in the wings and legs. In addition, GcanCSP4 was the highest expression on the female antennae at 12 d. These findings laid the foundation for further research on the mechanism of G. cantor olfactory mechanism at the molecular level.

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Section: Introductionmentioning

confidence: 99%

Antennal Transcriptome Analysis and Identification of Olfactory Genes in Glenea cantor Fabricius (Cerambycidae: Lamiinae)

Ouyang

et al. 2022

Insects

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“…C. medinalis, a number of olfactory genes were also mainly expressed in male antennae (Zhang et al, 2015;Liu et al, 2017bLiu et al, , 2020Sun et al, 2017;Li et al, 2020;Qu et al, 2020;Yang et al, 2020;He et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

“…The male antennae-biased expression suggests that these genes may play a role in the recognition of volatiles from females and/or beehives. In other insect species, including E. obliqua , O. furnacalis , Cotesia vestalis , Laodelphax striatellus , Leptocorisa acuta , Histia rhodope , Phthorimaea operculella , and C. medinalis , a number of olfactory genes were also mainly expressed in male antennae ( Zhang et al, 2015 ; Liu et al, 2017b , 2020 ; Sun et al, 2017 ; Li et al, 2020 ; Qu et al, 2020 ; Yang et al, 2020 ; He et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Identification of Olfactory Genes From the Greater Wax Moth by Antennal Transcriptome Analysis

et al. 2021

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The olfactory system is used by insects to find hosts, mates, and oviposition sites. Insects have different types of olfactory proteins, including odorant-binding proteins (OBPs), chemosensory proteins (CSPs), odorant receptors (ORs), ionotropic receptors (IRs), and sensory neuron membrane proteins (SNMPs) to perceive chemical cues from the environment. The greater wax moth, Galleria mellonella, is an important lepidopteran pest of apiculture. However, the molecular mechanism underlying odorant perception in this species is unclear. In this study, we performed transcriptome sequencing of G. mellonella antennae to identify genes involved in olfaction. A total of 42,544 unigenes were obtained by assembling the transcriptome. Functional classification of these unigenes was determined by searching against the Gene Ontology (GO), eukaryotic orthologous groups (KOG), and the Kyoto Encyclopedia of Genes and Genomes (KEGG) databases. We identified a total of 102 olfactory-related genes: 21 OBPs, 18 CSPs, 43 ORs, 18 IRs, and 2 SNMPs. Results from BLASTX best hit and phylogenetic analyses showed that most of the genes had a close relationship with orthologs from other Lepidoptera species. A large number of OBPs and CSPs were tandemly arrayed in the genomic scaffolds and formed gene clusters. Reverse transcription-quantitative PCR results showed that GmelOBP19 and GmelOR47 are mainly expressed in male antennae. This work provides a transcriptome resource for olfactory genes in G. mellonella, and the findings pave the way for studying the function of these genes.

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“…The prediction of the whole OBP family in species became quite simple due to the availability of more insect genomes and transcriptomes using next-generation sequencing techniques. However, the number of OBPs in Hymenoptera varies; for example, Apis mellifera has 21 OBPs, Microplitis mediator has 18 OBPs, Pieris rapae has 14 OBPs, Spodoptera exigua has 34 OBPs, Cotesia vestalis has 20 OBPs, and 90 OBPs were predicted in Nasonia vitripennis ( Forêt and Maleszka, 2006 ; Vieira et al, 2012 ; Liu et al, 2015 ; Peng et al, 2017 ; Li et al, 2020 ; Liu et al, 2020 ; Zhou et al, 2021 ). Insect OBPs not only are expressed in the chemosensory system, but also occur in nonsensory tissues and organs, such as the cornicles ( Wang et al, 2021a ), thoraxes ( Xue et al, 2016 ; Gao et al, 2018 ; Wang et al, 2019 ), reproductive organs ( Li et al, 2008 ; Sun et al, 2012b ), mandibular glands ( Iovinella et al, 2011 ), salivary glands ( Zhang et al, 2017 ), and wings ( Calvello et al, 2003 ; Pelosi et al, 2005 ; Wang et al, 2021b ).…”

Section: Introductionmentioning

confidence: 99%

Spatial Expression Analysis of Odorant Binding Proteins in Both Sexes of the Aphid Parasitoid Aphidius gifuensis and Their Ligand Binding Properties

et al. 2022

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In China, Aphidius gifuensis is one of the most common endoparasitoids of the green peach aphid Myzus persicae and grain aphid Sitobion miscanthi in the field. Insect odorant-binding proteins (OBPs) play vital roles in odor perception during feeding, host searching, mating and oviposition. In addition, some OBPs are involved in other physiological processes such as gustation and reproduction. In the present study, a comparative antennal transcriptomic analysis was applied between male and female A. gifuensis. The spatial expression patterns among antennae, heads, thoraxes, abdomens and legs of OBPs in both sexes were further profiled. Fifteen AgifOBPs were predicted, and 14 of them were identified by gene cloning, including 12 classic OBPs and 2 min-C OBPs. As expected, all OBPs were mainly expressed at high levels in antennae, heads or legs which are sensory organs and tissues. Finally, ligand binding properties of 2 OBPs (AgifOBP7 and AgifOBP9) were further evaluated. Female leg specifically expressed AgifOBP9 displays a broad and high binding property to aphid alarm pheromones, plant green volatiles and aphid sex pheromones (Ki < 10 μΜ). However, female leg specifically expressed AgifOBP7 displays poor affinity for all tested ligands except CAU-II-11 ((E)-3,7-dimethylocta-2,6-dien-1-yl-2-hydroxy-3-methoxybenzoate), a reported (E)-β-farnesene (EBF) analog with an exceptionally high binding affinity (Ki = 1.07 ± 0.08 μΜ). In summary, we reported the spatial expression pattern of the OBP repertoire in A. gifuensis, and further studied the binding properties of OBP7 and OBP9, which are mainly expressed in female legs, laying the foundation for the dissection of the contribution of OBPs to chemosensation in A. gifuensis.

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Identification and sex-biased profiles of candidate olfactory genes in the antennal transcriptome of the parasitoid wasp Cotesia vestalis

Cited by 22 publications

References 79 publications

Antennal Transcriptome Analysis and Identification of Olfactory Genes in Glenea cantor Fabricius (Cerambycidae: Lamiinae)

Antennal Transcriptome Analysis and Identification of Olfactory Genes in Glenea cantor Fabricius (Cerambycidae: Lamiinae)

Identification of Olfactory Genes From the Greater Wax Moth by Antennal Transcriptome Analysis

Spatial Expression Analysis of Odorant Binding Proteins in Both Sexes of the Aphid Parasitoid Aphidius gifuensis and Their Ligand Binding Properties

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