Identification and sequencing of remnant messenger RNAs found in domestic swine (Sus scrofa) fresh ejaculated spermatozoa

Yang, C. C.; Lin, Yusheng; Hsu, Ching‐Chi; Wu, Shinn-Chih; Lin, Eric S.; Cheng, W. T. K.

doi:10.1016/j.anireprosci.2008.08.012

Cited by 57 publications

(52 citation statements)

References 51 publications

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“…The presence of coding RNAs in sperms of humans (Lambard et al 2004), pigs (Yang et al 2009), bovines (Gilbert et al 2007, Lalancette et al 2008, mice (Fang et al 2014) and stallions (Das et al 2013) is well established. Even though the functions of the majority of the sperm mRNAs remain enigmatic, it has been proposed that sperm mRNAs may play roles in the regulation of sperm morphology, motility and capacitation and that sperm transcriptional profiles might provide clinical markers for male fertility (Lambard et al 2004, Li et al 2007, Bansal et al 2015.…”

Section: Introductionmentioning

confidence: 99%

Comparative profiling of small RNAs of pig seminal plasma and ejaculated and epididymal sperm

Cai¹,

Wu²,

Shen³

et al. 2017

Reproduction

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The similarities and differences of small RNAs in seminal plasma, epididymal sperm and ejaculated sperm remain largely undefined. We conducted a systematic comparative analysis of small RNA profiles in pig ejaculated sperm, epididymal sperm and seminal plasma and found that the diversity distribution of small RNA species was generally similar, whereas the abundance of small RNAs is dramatically different across the three libraries; miRNAs and small RNAs derived from rRNA, tRNA, small nuclear RNA, 7SK RNA, NRON RNA and cis-regulatory RNA were enriched in the three libraries, but piRNA was absent. A large population of small RNAs from ejaculated sperm are ejaculated sperm specific, and only 8-30% of small RNAs overlapped with those of epididymal sperm or seminal plasma and a small proportion (5-18%) of small RNAs were shared in the three libraries, suggesting that, in addition to the testes, sperm RNAs may also originate from seminal plasma, epididymis as well as other resources. Most miRNAs were co-distributed but differentially expressed across the three libraries, with epididymal sperm exhibiting the highest abundance, followed by ejaculated sperm and seminal plasma. The prediction of target genes of the top 10 highly expressed miRNAs across the three libraries revealed that these miRNAs may be involved in spermatogenesis, zygote development and the interaction between the environment and animals. Our study provides the first description of the similarities and differences of small RNA profiles in ejaculated sperm, epididymal sperm and seminal plasma and indicates that sperm RNA may have origins other than the testes.Reproduction (2017) 153 [785][786][787][788][789][790][791][792][793][794][795][796]

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Section: Introductionmentioning

confidence: 99%

Comparative profiling of small RNAs of pig seminal plasma and ejaculated and epididymal sperm

Cai¹,

Wu²,

Shen³

et al. 2017

Reproduction

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“…For example, Jasko et al (1990a) and findings provided a better concept of the biological importance of sperm RNAs, allowing the identification of biomarkers of stallion fertility. The sperm transcriptome has been studied in humans (Ostermeier et al 2002), boars (Kempisty et al 2008;Yang et al 2009) and bulls (Gilbert et al 2007;Bissonnette et al 2009;Feugang et al 2010), and the utility of sperm RNA as a marker of infertility has been explored (Miller 2000;Steger 2001), whereby differences in transcript levels in spermatozoa with different motilities (Bissonnette et al 2009;Lambard et al 2004), as well as between normal and abnormal sperm samples (Platts et al 2007;Steger et al 2003), have been reported. Krawetz (2005) demonstrated that some of the sperm RNAs can be delivered to the oocyte during fertilisation.…”

Section: Introductionmentioning

confidence: 99%

Implication of transcriptome profiling of spermatozoa for stallion fertility

Suliman

Becker

Wimmers

2018

Reprod. Fertil. Dev.

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Abstract. Poor fertility of breeding stallions is a recognised problem in the equine industry. The aim of the present study was to detect molecular pathways using two groups of stallions that differed in pregnancy rates as well as in the proportion of normal and motile spermatozoa. RNA was isolated from spermatozoa of each stallion and microarray data were analysed to obtain a list of genes for which transcript abundance differed between the groups (P #0.05, fold change $1.2). In all, there were 437 differentially expressed (DE) genes between the two groups (P # 0.05, fold change $1.2). Next, the DE genes were analysed using Database for Annotation, Visualisation, and Integrated Discovery (DAVID). Finally, ingenuity pathways analysis (IPA) was used to identify top biological functions and significant canonical pathways associated with the DE genes. Analysis using the DAVID database showed significant enrichment in the gene ontology (GO) term 'RNA binding' (P ¼ 0.05) and in the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway cytokinecytokine receptor interaction (P ¼ 0.02). Furthermore, IPA analysis showed interconnected biological functions and canonical pathways involved in the regulation of spermatogenesis and male fertility. In addition, significantly enriched metabolic pathways were identified. In conclusion, the present study has identified, for the first time, molecular processes in stallion spermatozoa that could be associated with stallion fertility.

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“…However, recent evidence suggests that a complex specific mRNA population is retained in freshly ejaculated spermatozoa of several species [1][2][3][4]. In addition, some spermatozoa mRNAs are transferred into the oocyte during fertilization, and remain stable until the activation of the embryonic genome [5].…”

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The role of Dby mRNA in early development of male mouse zygotes

Yao¹,

Xu²,

Gong³

et al. 2010

Asian J Androl

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Ejaculated mammalian spermatozoa contain a complex yet specific population of mRNA. However, the possible roles that mRNA has in early zygotic and embryonic development remain unclear. We found that Dby mRNA is selectively retained in capacitated mouse spermatozoa, and is transferred into the oocyte during fertilization by reverse transcription-polymerase chain reaction even though no DBY protein expression is detected. The cellular location of Dby mRNA is seen in the post-acrosome region, and it comprises nearly half of the mouse spermatozoa in in situ hybridization. In contrast, transcripts of the control gene, Smcy, are not detected in capacitated mouse spermatozoa, although the H-Y antigen encoded by Smcy is expressed on the surface of the spermatozoa. In our microinjection experiment, the zygotic development rate of the as-Dby male pronucleus injection group was significantly lower than that of the as-Smcy male pronucleus injection group (35.9% vs. 95%, P = 0.001) and the as-Dby female pronucleus injection group (35.9% vs. 93.8%, P = 0.001). The rate of male-developed zygotes was also lower than that of the as-Smcy male pronucleus injection group (17.4% vs. 57.9%, P = 0.002) and the as-Dby female pronucleus injection group (17.4% vs. 54.1%, P = 0.002). Thus, we conclude that Dby mRNA is selectively retained in capacitated mouse spermatozoa, and it has an important role in the early zygotic development of male mouse zygotes. This might imply that spermatozoa mRNA is involved in early zygotic and embryonic stages of reproduction.

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Identification and sequencing of remnant messenger RNAs found in domestic swine (Sus scrofa) fresh ejaculated spermatozoa

Cited by 57 publications

References 51 publications

Comparative profiling of small RNAs of pig seminal plasma and ejaculated and epididymal sperm

Comparative profiling of small RNAs of pig seminal plasma and ejaculated and epididymal sperm

Implication of transcriptome profiling of spermatozoa for stallion fertility

The role of Dby mRNA in early development of male mouse zygotes

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