Identification and sequencing of ESTs from the halophyte grass Aeluropus littoralis

Zouari, Nabil; Saad, Rania Ben; Legavre, Thierry; Azaza, Jalel; Sabau, Xavier; Jaoua, Samir; Masmoudi, Khaled; Hassairi, Afif

doi:10.1016/j.gene.2007.08.021

Cited by 59 publications

(62 citation statements)

References 55 publications

(64 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This gene showed high homology with Hordeum vulgare (Shi et al, 2001). We found the thioredoxin M and H3 type in our database, which have earlier been shown to be associated with (Zouari et al, 2007), Thellungiella (Wong et al, 2005). The GST gene is made full length and the sequence has been submitted in the NCBI GenBank (Accession number EF373062) and further being analyzed for its potential role in salt tolerance.…”

Section: Functional Classification Of Salt Related Estsmentioning

confidence: 96%

“…Arabidopsis genome encodes approximately 5.9% transcription factors (Riechmann et al, 2000). In different halophytes transcription factors form an important component of EST database as evident in Aeluropus littoralis (Zouari et al, 2007), Mesembryanthemum (Kore-eda et al, 2004), Suaeda (Zhang et al, 2001), Thellungiella (Wong et al, 2005). In Thellungiella, Wong et al (2005) have reported that 1% genes belong to transcription or signal transduction elements.…”

Section: Functional Classification Of Salt Related Estsmentioning

confidence: 99%

See 1 more Smart Citation

Identification of salt-induced genes from Salicornia brachiata, an extreme halophyte through expressed sequence tags analysis

et al. 2009

View full text Add to dashboard Cite

Salinity severely affects plant growth and development causing crop loss worldwide. We have isolated a large number of salt-induced genes as well as unknown and hypothetical genes from Salicornia brachiata Roxb. (Amaranthaceae). This is the first description of identification of genes in response to salinity stress in this extreme halophyte plant. Salicornia accumulates salt in its pith and survives even at 2 M NaCl under field conditions. For isolating salt responsive genes, cDNA subtractive hybridization was performed between control and 500 mM NaCl treated plants. Out of the 1200 recombinant clones, 930 sequences were submitted to the NCBI database (GenBank accession: EB484528 to EB485289 and EC906125 to EC906292). 789 ESTs showed matching with different genes in NCBI database. 4.8% ESTs belonged to stress-tolerant gene category and approximately 29% ESTs showed no homology with known functional gene sequences, thus classified as unknown or hypothetical. The detection of a large number of ESTs with unknown putative function in this species makes it an interesting contribution. The 90 unknown and hypothetical genes were selected to study their differential regulation by reverse Northern analysis for identifying their role in salinity tolerance. Interestingly, both up and down regulation at 500 mM NaCl were observed (21 and 10 genes, respectively). Northern analysis of two important salt tolerant genes, ASR1 (Abscisic acid stress ripening gene) and plasma membrane H + ATPase, showed the basal level of transcripts in control condition and an increase with NaCl treatment. ASR1 gene is made full length using 5' RACE and its potential role in imparting salt tolerance is being studied.

show abstract

Section: Functional Classification Of Salt Related Estsmentioning

confidence: 96%

Section: Functional Classification Of Salt Related Estsmentioning

confidence: 99%

Identification of salt-induced genes from Salicornia brachiata, an extreme halophyte through expressed sequence tags analysis

et al. 2009

View full text Add to dashboard Cite

show abstract

“…What appears to emerge is the existence of a small number of novel genes (Vinocur and Altman, 2005), while novel uses of kingdom-wide existing genes and proteins seem to provide the bulk of the mechanisms that form the basis of the stress-tolerance phenotype. Increasingly, halophyte transcriptomes are being analyzed and contrasted to glycophytic relatives (Kore-eda et al, 2004;Gong et al, 2005;Zouari et al, 2007;Dassanayake et al, 2009;Guo et al, 2009;Chelaifa et al, 2010). With the advent of numerous ongoing whole genome sequencing projects and comparisons throughout the plant kingdom, a multifaceted approach becomes possible.…”

Section: Toward the T Parvula Genome Sequencementioning

confidence: 99%

Genome Structures and Halophyte-Specific Gene Expression of the Extremophile Thellungiella parvula in Comparison with Thellungiella salsuginea (Thellungiella halophila) and Arabidopsis

Dassanayake

et al. 2010

Plant Physiology

View full text Add to dashboard Cite

The genome of Thellungiella parvula, a halophytic relative of Arabidopsis (Arabidopsis thaliana), is being assembled using Roche-454 sequencing. Analyses of a 10-Mb scaffold revealed synteny with Arabidopsis, with recombination and inversion and an uneven distribution of repeat sequences. T. parvula genome structure and DNA sequences were compared with orthologous regions from Arabidopsis and publicly available bacterial artificial chromosome sequences from Thellungiella salsuginea (previously Thellungiella halophila). The three-way comparison of sequences, from one abiotic stress-sensitive species and two tolerant species, revealed extensive sequence conservation and microcolinearity, but grouping Thellungiella species separately from Arabidopsis. However, the T. parvula segments are distinguished from their T. salsuginea counterparts by a pronounced paucity of repeat sequences, resulting in a 30% shorter DNA segment with essentially the same gene content in T. parvula. Among the genes is SALT OVERLY SENSITIVE1 (SOS1), a sodium/proton antiporter, which represents an essential component of plant salinity stress tolerance. Although the SOS1 coding region is highly conserved among all three species, the promoter regions show conservation only between the two Thellungiella species. Comparative transcript analyses revealed higher levels of basal as well as salt-induced SOS1 expression in both Thellungiella species as compared with Arabidopsis. The Thellungiella species and other halophytes share conserved pyrimidine-rich 5# untranslated region proximal regions of SOS1 that are missing in Arabidopsis. Completion of the genome structure of T. parvula is expected to highlight distinctive genetic elements underlying the extremophile lifestyle of this species.

show abstract

“…Identification and isolation of novel genes by genomic approaches will advance the understanding of these mechanisms [24]. Hence, different efforts in large-scale ESTsequencing and analysis have been made in a number of dicotyledonous halophytes, such as Suaeda salsa [25], Thellungiella halophila [26], Mesembryanthemum crystallinum [27], Aegiceras corniculatum [28], Avicennia marina [29], Tamarix hispida [30], and Limonium sinense [31], and monocotyledonous halophytes such as Leymus chinensis [32], Puccinellia tenuiflora [33], Lolium temulentum [34], Aeluropus littoralis [35], and Spartina alterniflora [36].…”

Section: Halophytes and Their Adaptations To Salinitymentioning

confidence: 99%

Molecular Breeding for Enhancing Abiotic Stress Tolerance Using Halophytes

Parida

George

Kavita

2012

Improving Crop Resistance to Abiotic Stress

View full text Add to dashboard Cite

Identification and sequencing of ESTs from the halophyte grass Aeluropus littoralis

Cited by 59 publications

References 55 publications

Identification of salt-induced genes from Salicornia brachiata, an extreme halophyte through expressed sequence tags analysis

Identification of salt-induced genes from Salicornia brachiata, an extreme halophyte through expressed sequence tags analysis

Genome Structures and Halophyte-Specific Gene Expression of the Extremophile Thellungiella parvula in Comparison with Thellungiella salsuginea (Thellungiella halophila) and Arabidopsis

Molecular Breeding for Enhancing Abiotic Stress Tolerance Using Halophytes

Contact Info

Product

Resources

About