Identification and sequence of a Na+‐linked gene from the marine bacterium Alteromonas haloplanktis which functionally complements the dagA gene of Escherichia coli

MacLeod, Patricia; MacLeod, Robert A.

doi:10.1111/j.1365-2958.1992.tb01444.x

Cited by 15 publications

(11 citation statements)

References 42 publications

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“…S2 in the supplemental material). The riboswitch has structural and sequence homology to other glycine riboswitches (28), and AA00167 belongs to the sodium/alanine symporter superfamily (35), which includes a known glycine transporter (34). We also observed high levels of an sRNA homologous to GcvB in A. actinomycetemcomitans biofilms.…”

Section: Discussionmentioning

confidence: 54%

Characterization of a Novel Riboswitch-Regulated Lysine Transporter inAggregatibacter actinomycetemcomitans

Jorth

Whiteley

2010

J Bacteriol

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Aggregatibacter actinomycetemcomitans is an opportunistic pathogen that resides primarily in the mammalian oral cavity. In this environment, A. actinomycetemcomitans faces numerous host-and microbederived stresses, including intense competition for nutrients and exposure to the host immune system. While it is clear that A. actinomycetemcomitans responds to precise cues that allow it to adapt and proliferate in the presence of these stresses, little is currently known about the regulatory mechanisms that underlie these responses. Many bacteria use noncoding regulatory RNAs (ncRNAs) to rapidly alter gene expression in response to environmental stresses. Although no ncRNAs have been reported in A. actinomycetemcomitans, we propose that they are likely important for colonization and persistence in the oral cavity. Using a bioinformatic and experimental approach, we identified three putative metabolitesensing riboswitches and nine small regulatory RNAs (sRNAs) in A. actinomycetemcomitans during planktonic and biofilm growth. Molecular characterization of one of the riboswitches revealed that it is a lysine riboswitch and that its target gene, lysT, encodes a novel lysine-specific transporter. Finally, we demonstrated that lysT and the lysT lysine riboswitch are conserved in over 40 bacterial species, including the phylogenetically related pathogen Haemophilus influenzae.

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Section: Discussionmentioning

confidence: 54%

Characterization of a Novel Riboswitch-Regulated Lysine Transporter inAggregatibacter actinomycetemcomitans

Jorth

Whiteley

2010

J Bacteriol

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“…6) revealed 50% homology between Orf3 and the D-alanine permease (DagA) of the marine bacterium Alteromonas haloplanktis (26) and 71% homology between Orf1 and the YadQ protein of E. coli, whose function has not been defined (14). Low homologies to eukaryotic chloride channels and the Gef1 protein of Saccharomyces cerevisiae, were found; a relationship to iron and citrate has been found for this protein (10).…”

Section: Discussionmentioning

confidence: 99%

Ferric rhizoferrin uptake into Morganella morganii: characterization of genes involved in the uptake of a polyhydroxycarboxylate siderophore

1996

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Iron uptake in Morganella morganii, mediated by the fungal siderophore rhizoferrin, was studied. A Mud1 insertion mutant devoid of growth on ferric rhizoferrin was complemented by a chromosomal DNA fragment of M. morganii that encoded an outer membrane protein and a periplasmic protein named RumA and RumB (for rhizoferrin uptake into Morganella spp.), respectively. rumA and rumB have the same transcription polarity and are probably cotranscribed from an iron-regulated promoter upstream of rumA. A predicted Fur regulatory sequence upstream of rumA was confirmed by the Fur titration assay. At the N terminus of RumA, a putative TonB box contains a proline residue that inactivates TonB-dependent receptors and colicins when introduced at the same position into TonB boxes of Escherichia coli. Analysis of a 10-kb sequence flanking rumA and rumB on both sides revealed seven additional open reading frames for which no role in ferric rhizoferrin uptake could be discerned. Thus, rumA and rumB, both essential for transport of this siderophore, form an isolated operon. Additional genes required for ferric rhizoferrin translocation across the cytoplasmic membrane must map at sites distinct from rumA and rumB.

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“…The search for similar proteins in the databases gave a few positive results. Several of them do not have a known function, but two of the other proteins are sodium/proton-dependent alanine carrier proteins (MacLeod & MacLeod, 1992;Kamata e t al., 1992). The transmembrane domains and the similarities to known proteins are indications of a possible amino acid carrier function for this protein.…”

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confidence: 99%

New genes in the 170 region of the Bacillus subtilis genome encode DNA gyrase subunits, a thioredoxin, a xylanase and an amino acid transporter

Rose

Entian²

1996

Microbiology

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A DNA contig of 26.2 kb covering the 170" region of the Bacillus subtilis strain 168 genome was isolated and sequenced. For DNA isolation, suitable restriction sites at the end of previously known genes were chosen to amplify adjacent unknown DNA regions by inverse PCR. On the basis of the DNA sequence, 26 ORFs were identified of which eg/S and ccd!A, as well as parts of citB and tkf have been described previously. Here we report the complete sequences of the aconitase (citl?) and transketolase (tkt) genes. Of the other proteins encoded on the 262 kb fragment, eight revealed similarities t o previously described proteins. These included a pair of newly identified DNA gyrase subunits A (grlA) and B (gr/B), a sodium/proton-dependent alanine carrier (alsn, a member of the thioredoxin family @/PA), an endo-1,4+ xylanase (XUnD) and a response regulator protein. Comparison of the physical and the genetic maps revealed several differences. According to i t s flanking sequences the leXA (din!?) gene which was previously mapped at 162" was found to be adjacent t o yneA localized at 170". Genes citi3 and eg/S were located the opposite way round and closer together than expected from the genetic map (citB at 173" and eglS at 170"). The pr&A gene, which was mapped at ISSO, was not present on the respective fragment. Sequence comparison actually showed that prkA is located close to 70" on the B. subfilis genome.

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Identification and sequence of a Na⁺‐linked gene from the marine bacterium Alteromonas haloplanktis which functionally complements the dagA gene of Escherichia coli

Cited by 15 publications

References 42 publications

Characterization of a Novel Riboswitch-Regulated Lysine Transporter inAggregatibacter actinomycetemcomitans

Characterization of a Novel Riboswitch-Regulated Lysine Transporter inAggregatibacter actinomycetemcomitans

Ferric rhizoferrin uptake into Morganella morganii: characterization of genes involved in the uptake of a polyhydroxycarboxylate siderophore

New genes in the 170 region of the Bacillus subtilis genome encode DNA gyrase subunits, a thioredoxin, a xylanase and an amino acid transporter

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