Identification and response of cytochrome P450 genes in the brackish water flea Diaphanosoma celebensis after exposure to benzo[α]pyrene and heavy metals

Han, Jeonghoon; Lee, Kyun-Woo

doi:10.1007/s11033-020-06113-y

Cited by 7 publications

(2 citation statements)

References 49 publications

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“…The induction of CYP enzymes may be an adaptive response to metal exposure, whereas their decrease inhibits detoxification [ 97 ]. In Cu 2+ -exposed Diaphanosoma celebensis , CYP-related genes, including cyp2 and cyp4 , were found to be upregulated at an earlier exposure time (6 h) but downregulated at a later exposure time (24h) [ 98 ]. In C. auratus , Cu 2+ exposure upregulated the expression of cyp1a and cyp3a , but combined treatment with Cu 2+ and diclofenac decreased their expression [ 99 ].…”

Section: Discussionmentioning

confidence: 99%

Effects of Copper Exposure on Oxidative Stress, Apoptosis, Endoplasmic Reticulum Stress, Autophagy and Immune Response in Different Tissues of Chinese Mitten Crab (Eriocheir sinensis)

Feng

Song

et al. 2022

Antioxidants

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High concentrations of copper (Cu2+) pose a great threat to aquatic animals. However, the mechanisms underlying the response of crustaceans to Cu2+ exposure have not been well studied. Therefore, we investigated the alterations of physiological and molecular parameters in Chinese mitten crab (Eriocheir sinensis) after Cu2+ exposure. The crabs were exposed to 0 (control), 0.04, 0.18, and 0.70 mg/L of Cu2+ for 5 days, and the hemolymph, hepatopancreas, gills, and muscle were sampled. The results showed that Cu2+ exposure decreased the antioxidative capacity and promoted lipid peroxidation in different tissues. Apoptosis was induced by Cu2+ exposure, and this activation was associated with the mitochondrial and ERK pathways in the hepatopancreas. ER stress-related genes were upregulated in the hepatopancreas but downregulated in the gills at higher doses of Cu2+. Autophagy was considerably influenced by Cu2+ exposure, as evidenced by the upregulation of autophagy-related genes in the hepatopancreas and gills. Cu2+ exposure also caused an immune response in different tissues, especially the hepatopancreas, where the TLR2-MyD88-NF-κB pathway was initiated to mediate the inflammatory response. Overall, our results suggest that Cu2+ exposure induces oxidative stress, ER stress, apoptosis, autophagy, and immune response in E. sinensis, and the toxicity may be implicated following the activation of the ERK, AMPK, and TLR2-MyD88-NF-κB pathways.

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Section: Discussionmentioning

confidence: 99%

Effects of Copper Exposure on Oxidative Stress, Apoptosis, Endoplasmic Reticulum Stress, Autophagy and Immune Response in Different Tissues of Chinese Mitten Crab (Eriocheir sinensis)

Feng

Song

et al. 2022

Antioxidants

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“…By contrast, CYP1A has not been observed in invertebrates. Recent studies in marine invertebrates reported the involvement of CYPs from different families in detoxi cation in response to xenobiotics (Kim et al 2013b;Yim et al 2017;Han and Lee 2021). Several CYPs were also identi ed in marine polychaetes, and their functions were analyzed by mRNA expression studies.…”

Section: Introductionmentioning

confidence: 99%

Identification of a novel CYP4V gene in the polychaete Perinereis aibuhitensis: transcriptional comparison with a CYP4B gene exposed to PAHs

Zhao

Wang

Yan

et al. 2022

Environ Sci Pollut Res

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Polychaete worms can biotransform polycyclic aromatic hydrocarbons (PAHs) in environments, and the cytochrome P450 (CYP) enzyme plays an important role in this process. Herein, a novel cytochrome P450 gene was identi ed and characterized from the polychaete worm Perinereis aibuhitensis. The full-length cDNA, which is named CYP4V82, is 1709 bp encoding a protein of 509 amino acids and has high similarity to CYP4V. The expression levels of CYP4V82 and CYP4BB4 (a CYP gene identi ed from P. aibuhitensis in a previous study, Chen et al., 2012) exposure to various concentrations of benzo[α]pyrene (B[α]P) (0.5, 2, 4, and 8 μg/L) and same mass concentrations of uoranthene (Flu, 3.2 μg/L), phenanthrene (Phe, 2.9 μg/L), B[α]P (4.0 μg/L) were detected to identify the function of the CYP4 family in P. aibuhitensis. Compared with CYP4BB4, CYP4V82 mRNA was minimally expressed on day 7 but highly sensitive on day 14. Notably, the expression level of CYP4V82 and CYP4BB4 was relatively different in response to PAHs with different benzene rings of the same concentration. The expression of CYP4V82 in the B(a)P group was the highest, while that of CYP4BB4 in the Phe group was relatively higher than the two other groups. These findings suggest that PAHs are associated with the induction of CYP4V82 and CYP4BB4 expressions, which may have different e ciencies in the detoxification of PAHs.

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Characterization of the molecular differential responses in marine benthic macroinvertebrates exposed to polycyclic aromatic hydrocarbons

Onyena,

Manohar,

Nkwoji

et al. 2023

Aquat Ecol

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Identification and response of cytochrome P450 genes in the brackish water flea Diaphanosoma celebensis after exposure to benzo[α]pyrene and heavy metals

Cited by 7 publications

References 49 publications

Effects of Copper Exposure on Oxidative Stress, Apoptosis, Endoplasmic Reticulum Stress, Autophagy and Immune Response in Different Tissues of Chinese Mitten Crab (Eriocheir sinensis)

Effects of Copper Exposure on Oxidative Stress, Apoptosis, Endoplasmic Reticulum Stress, Autophagy and Immune Response in Different Tissues of Chinese Mitten Crab (Eriocheir sinensis)

Identification of a novel CYP4V gene in the polychaete Perinereis aibuhitensis: transcriptional comparison with a CYP4B gene exposed to PAHs

Characterization of the molecular differential responses in marine benthic macroinvertebrates exposed to polycyclic aromatic hydrocarbons

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