Identification and regulation of cold-inducible factors of Bordetella bronchiseptica

Stübs, Dorothee; Fuchs, Thilo M.; Schneider, Boris; Bosserhoff, Anja‐Katrin; Gross, Roy

doi:10.1099/mic.0.27785-0

Cited by 16 publications

(9 citation statements)

References 57 publications

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“…Nucleotide sequence comparison of Csps in the EGD-e strain revealed high levels of sequence similarity, with identities ranging from 77 to 84% (data not shown). All the csp open reading frames in this organism are preceded by relatively long stretches of 5Ј untranslated leader regions (5 ЈUTR) ranging from 198 bp (cspA) to 363 bp (cspD), a situation which is similar to what has previously been described for some csp genes of other bacterial species (1,34). These long 5Ј UTR regions were predicted to be rich in RNA hairpin loop secondary structures (DNASIS MaxVersion 2.6 program; Miraibio, San Francisco, CA), which might have implications for the regulation of csp gene expression in this organism.…”

Section: Resultssupporting

confidence: 67%

“…The potential role of Csps in osmotic stress adaptation so far stems from the fact that expression of the cspC gene in Bordetella bronchiseptica is significantly induced following exposure of this organism to 2 M NaCl (34). We show here that direct growth of L. monocytogenes EGD-e under NaCl stress in BHI-NaCl leads to significant induction of cspA and cspD gene expression, although the overall NaCl stress-associated csp inductions appeared lower compared to those in cold-adapted cells.…”

Section: Discussionmentioning

confidence: 60%

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Role of Cold Shock Proteins in Growth of Listeria monocytogenes under Cold and Osmotic Stress Conditions

Schmid

Klumpp

Raimann

et al. 2009

Appl Environ Microbiol

173

186

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The gram-positive bacterium Listeria monocytogenes is a food-borne pathogen of both public health and food safety significance. It possesses three small, highly homologous protein members of the cold shock protein (Csp) family. We used gene expression analysis and a set of mutants with single, double, and triple deletions of the csp genes to evaluate the roles of CspA, CspB, and CspD in the cold and osmotic (NaCl) stress adaptation responses of L. monocytogenes. All three Csps are dispensable for growth at optimal temperature (37°C). These proteins are, however, required for efficient cold and osmotic stress tolerance of this bacterium. The hierarchies of their functional importance differ, depending on the environmental stress conditions: CspA>CspD>CspB in response to cold stress versus CspD>CspA/CspB in response to NaCl salt osmotic stress. The fact that Csps are promoting L. monocytogenes adaptation against both cold and NaCl stress has significant implications in view of practical food microbial control measures. The combined or sequential exposure of L. monocytogenes cells to these two stresses in food environments might inadvertently induce cross-protection responses.

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Section: Resultssupporting

confidence: 67%

Section: Discussionmentioning

confidence: 60%

Role of Cold Shock Proteins in Growth of Listeria monocytogenes under Cold and Osmotic Stress Conditions

Schmid

Klumpp

Raimann

et al. 2009

Appl Environ Microbiol

173

186

View full text Add to dashboard Cite

show abstract

“…Upregulation of a gene encoding cold shock-like protein CspD ( vp1012 ) was also detected. Cold-shock proteins can regulate a wide range of cellular functions in bacteria, including virulence factors; in Bordetella bronchiseptica the cspC and cspD genes appear to be repressed by the virulence regulatory BvgAS two-component system, while in Listeria monocytogenes Csps have been found to regulate hemolysis and listeriolysin production (Stübs et al, 2005; Schärer et al, 2013). To the author's knowledge, the role of Csps in V. parahaemolyticus virulence has not been investigated to date.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome analysis of Vibrio parahaemolyticus in type III secretion system 1 inducing conditions

Nydam

Shah

Call

2014

Front. Cell. Infect. Microbiol.

130

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Vibrio parahaemolyticus is an emerging bacterial pathogen capable of causing inflammatory gastroenteritis, wound infections, and septicemia. As a food-borne illness, infection is most frequently associated with the consumption of raw or undercooked seafood, particularly shellfish. It is the primary cause of Vibrio-associated food-borne illness in the United States and the leading cause of food-borne illness in Japan. The larger of its two chromosomes harbors a set of genes encoding type III section system 1 (T3SS1), a virulence factor present in all V. parahaemolyticus strains that is similar to the Yersinia ysc T3SS. T3SS1 translocates effector proteins into eukaryotic cells where they induce changes to cellular physiology and modulate host-pathogen interactions. T3SS1 is also responsible for cytotoxicity toward several different cultured cell lines as well as mortality in a mouse model. Herein we used RNA-seq to obtain global transcriptome patterns of V. parahaemolyticus under conditions that either induce [growth in Dulbecco's Modified Eagle Medium (DMEM) media, in trans expression of transcriptional regulator exsA] or repress T3SS1 expression (growth in LB-S media, in trans exsD expression) and during infection of HeLa cells over time. Comparative transcriptomic analysis demonstrated notable differences in the expression patterns under inducing conditions and was also used to generate an expression profile of V. parahaemolyticus during infection of HeLa cells. In addition, we identified several new genes that are associated with T3SS1 expression and may warrant further study.

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“…Of the nine genes tested, only the 16S rRNA gene was stably expressed at the two temperatures. Although housekeeping genes involved in basic cellular metabolism are usually constitutively expressed, some bacteria respond to low temperature by altering protein synthesis and cellular metabolism (6,52). Hence, the upregulation of seven of the remaining candidate reference genes was not unexpected.…”

Section: Discussionmentioning

confidence: 99%

Identification of Cold-Temperature-Regulated Genes in Flavobacterium psychrophilum

Hesami

Metcalf

Lumsden

et al. 2011

Appl Environ Microbiol

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Flavobacterium psychrophilum is the etiological agent of bacterial coldwater disease (BCWD) and rainbow trout fry syndrome (RTFS). It causes disease primarily in fresh water-reared salmonids, but other fish species can also be affected. A diverse array of clinical conditions is associated with BCWD, including tail rot (peduncle disease), necrotic myositis, and cephalic osteochondritis. Degradation of connective and muscular tissues by extracellular proteases is common to all of these presentations. There are no effective vaccines to prevent BCWD or RTFS, and antibiotics are often used to prevent and control disease. To identify virulence factors that might permit development of an efficacious vaccine, cDNA suppression subtractive hybridization (SSH) was used to identify cold-regulated genes in a virulent strain of F. psychrophilum. Genes predicted to encode a two-component system sensor histidine kinase (LytS), an ATP-dependent RNA helicase, a multidrug ABC transporter permease/ATPase, an outer membrane protein/protective antigen OMA87, an M43 cytophagalysin zinc-dependent metalloprotease, a hypothetical protein, and four housekeeping genes were upregulated at 8°C versus the level of expression at 20°C. Because no F. psychrophilum gene was known to be suitable as an internal standard in reverse transcription-quantitative real-time PCR (RT-qPCR) experiments, the expression stability of nine commonly used reference genes was evaluated at 8°C and 20°C. Expression of the 16S rRNA was equivalent at both temperatures, and this gene was used in RT-qPCR experiments to verify the SSH findings. With the exception of the ATCC 49513 strain, similar patterns of gene expression were obtained with 11 other representative strains of F. psychrophilum.Bacterial cold water disease (BCWD), caused by Flavobacterium psychrophilum, occurs at low water temperatures and can cause economic losses in the aquaculture industry as a result of direct mortality or vertebral deformities that decrease the market value of fish that survive the infection (31, 35). A number of putative virulence factors of this bacterium have been identified, including extracellular proteases involved in degradation of extracellular matrix components such as elastin, fibrinogen, type IV collagen, actin, and myosin (9,37,47,48). The best studied of these, psychrophilic metalloproteases Fpp1 and Fpp2, are thought to be involved in destruction of host tissues; however, their roles have not yet been demonstrated in vivo (47,48). In a recent study, Sudheesh et al. (53) used two-dimensional acrylamide gel electrophoresis and Western blot analysis to compare a virulent strain and a nonvirulent strain of F. psychrophilum and found a thermolysin that was unique to the virulent strain. A role for the iron uptake-associated gene exbD2 has also been demonstrated. Strains lacking this gene have decreased virulence and confer a high level of protection in rainbow trout fry when they are used for vaccination (4). Other putative virulence factors of F. psychrophilum include a lipo...

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Identification and regulation of cold-inducible factors of Bordetella bronchiseptica

Cited by 16 publications

References 57 publications

Role of Cold Shock Proteins in Growth of Listeria monocytogenes under Cold and Osmotic Stress Conditions

Role of Cold Shock Proteins in Growth of Listeria monocytogenes under Cold and Osmotic Stress Conditions

Transcriptome analysis of Vibrio parahaemolyticus in type III secretion system 1 inducing conditions

Identification of Cold-Temperature-Regulated Genes in Flavobacterium psychrophilum

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