2012
DOI: 10.1016/j.devcel.2012.05.007
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Identification and Regulation of a Molecular Module for Bleb-Based Cell Motility

Abstract: Single-cell migration is a key process in development, homeostasis, and disease. Nevertheless, the control over basic cellular mechanisms directing cells into motile behavior in vivo is largely unknown. Here, we report on the identification of a minimal set of parameters the regulation of which confers proper morphology and cell motility. Zebrafish primordial germ cells rendered immotile by knockdown of Dead end, a negative regulator of miRNA function, were used as a platform for identifying processes restorin… Show more

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Cited by 64 publications
(77 citation statements)
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References 47 publications
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“…By contrast, blebs contact a very different extracellular environment, including the basal lamina and the ECM of the peripheral mesenchyme, to which cells may adhere and thereby generate traction and forward movement. In primordial germ cells, blebs protrude forward and, as they retract, actomyosin coupled with adhesion pulls the cell forward (Goudarzi et al, 2012;Kardash et al, 2010). Our data are consistent with NCC blebs driving forward movement in a similar manner.…”
Section: Discussionsupporting
confidence: 79%
“…By contrast, blebs contact a very different extracellular environment, including the basal lamina and the ECM of the peripheral mesenchyme, to which cells may adhere and thereby generate traction and forward movement. In primordial germ cells, blebs protrude forward and, as they retract, actomyosin coupled with adhesion pulls the cell forward (Goudarzi et al, 2012;Kardash et al, 2010). Our data are consistent with NCC blebs driving forward movement in a similar manner.…”
Section: Discussionsupporting
confidence: 79%
“…Bleb formation was previously related to cortical actomyosin tension 26 . The observed 26,27 . Remarkably, in the otic vesicle, the rounding cells are immersed in a packed epithelium, ARTICLE such that the rounding severed cells are pushing over other cells to form the bleb.…”
Section: Articlementioning
confidence: 89%
“…However, if the cytoskeletal network is dense, a relatively long-lived pressure transient will develop, the cell membrane will detach from the actin cortex, and a bleb will form. Experimental support to this mechanism includes the observation that blebs can be induced by creating local pressure differences using micropipettes [16], by locally reducing the adhesion energy between the cell and its cortex [17], by locally ablating the actin cortex with a laser [18], by increasing cortical contractility [19], and by depolymerizing the cortex [20]. However, establishing a definitive causal link between membrane/cortex facture and a local increase in pressure of poroelastic origin remains a remarkable experimental challenge.…”
Section: Hydraulic Fracturing In Cells and Tissuesmentioning
confidence: 99%