Identification and Registration of Maize Genotypes with the Use of Molecular Markers

Kozhukhova, N. E.; Sivolap, Yu. M.

doi:10.1023/b:ruge.0000013448.70285.83

Cited by 5 publications

(2 citation statements)

References 8 publications

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“…A smaller number of markers, sufficient to determine the difference between the hybrids, indicated both greater diversity of the analysed hybrids and suitability of the selected markers for maize fingerprinting. Kozhukhova and Sivolap (2004) differentiated 40 unique molecular patterns of 40 maize genotypes with ten polymorphic SSR loci. In another study, 20 microsatellites revealed unique allelic combinations for 48 commercial maize single-cross hybrids, with a probability of identity ranging from 10 − 14 to 10 − 7 (Ribeiro et al, 2017).…”

Section: Locusmentioning

confidence: 99%

Molecular diversity and microsatellite polymorphism of modern maize hybrids

Mikić¹,

Brbaklić²,

Stanisavljević³

et al. 2018

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The aim of this study was to estimate genetic diversity of 97 modern maize hybrids cultivated in Serbia with 12 microsatellite markers. In total, 89 alleles were detected, with the average of 7.4 alleles per locus. The polymorphic information content ranged from 0.42, for umc1792, to 0.81, for dupssr10, with an average of 0.64. The pairwise relatedness Ritland's values varied between 0.787 and-0.129. Eight markers were sufficient to differentiate hybrids with unique genotypes. A principal coordinate analysis distinguished early and late maturing groups of hybrids, although the observed differentiation between groups was low (Fst = 0.4%). Two markers, bnlg1556 and umc1075, contributed most to the discrimination between early and late genotypes. The values of parameters of molecular genetic diversity were higher in early than in late maturing hybrids, indicating broader genetic basis of the former. A high level of polymorphism of the markers indicates their suitability for fingerprinting.

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Section: Locusmentioning

confidence: 99%

Molecular diversity and microsatellite polymorphism of modern maize hybrids

Mikić¹,

Brbaklić²,

Stanisavljević³

et al. 2018

Ratar i povrt

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“…Among the DNA markers, microsatellites or SSRs ("Simple Sequence Repeats") have additional attributes such as codominance and multiallelism that make them one of the best choices for the identification of genetic contamination. Sivolap and Kozhukhova (2004) showed that in the analysis of ten SSR loci, 40 genotypes were unique. For every locus, SSR analysis revealed heterozygotes among simple hybrids, which made it possible to identify the parental forms with a high probability of exclusion of nonparental forms.…”

Section: Introductionmentioning

confidence: 99%

Genetic purity certificate in seeds of hybrid maize using molecular markers

Salgado¹,

Vieira²,

Pinho³

et al. 2006

Rev. bras. sementes

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-One of the main features that confer high quality to the seed is its genetic purity, in which one of the major causes of contamination is the self-pollination of the female parent. Up to date, there is no accurate and fast methods for detecting such contamination. Thus, this work was carried out to certify the genetic purity in seeds of hybrid maize using different biochemical and DNA-based markers. Two single-cross hybrids and their parental lines derived from the maize breeding program at UFLA were evaluated by isoenzymatic pattern of alcohol dehydrogenase (ADH), esterase (EST), acid phosphatase (ACP), glutamate-oxaloacetate transaminase (GOT), malate dehydrogenase (MDH), isocitrate dehydrogenase (IDH), phosphoglucomutase (PGM), 6-phosphoglucomate dehydrogenase (PGDH), catalase (CAT) and ß-glucosidade (ßGLU) and by microsatellites markers. The enzymatic systems that were able to distinguish the hybrids from their parental line were the catalase, the isocitrate dehydrogenase and the esterase. The esterase showed a Mendelian segregation pattern for UFLA 8/3 hybrid, that enables a safer genetic purity certificate. Microsatellites were able to differentiate the hybrid lines and the respective parental lines. Moreover, this technique was fast, precise and without environment effects. For microsatellites, the amplification pattern was identical when young leaves or seeds were used as DNA source. The possibility of using seeds as DNA source would accelerate and facilitate the role process of the genetic purity analysis.

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Assessment of the influence of field size on maize gene flow using SSR analysis

et al. 2011

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One of the factors that may influence the rate of cross-fertilization is the relative size of the pollen donor and receptor fields. We designed a spatial distribution with four varieties of genetically-modified (GM) yellow maize to generate different sized fields while maintaining a constant distance to neighbouring fields of conventional white kernel maize. Samples of cross-fertilized, yellow kernels in white cobs were collected from all of the adjacent fields at different distances. A special series of samples was collected at distances of 0, 2, 5, 10, 20, 40, 80 and 120 m following a transect traced in the dominant down-wind direction in order to identify the origin of the pollen through SSR analysis. The size of the receptor fields should be taken into account, especially when they extend in the same direction than the GM pollen flow is coming. From collected data, we then validated a function that takes into account the gene flow found in the field border and that is very useful for estimating the % of GM that can be found in any point of the field. It also serves to predict the total GM content of the field due to cross fertilization. Using SSR analysis to identify the origin of pollen showed that while changes in the size of the donor field clearly influence the percentage of GMO detected, this effect is moderate. This study demonstrates that doubling the donor field size resulted in an approximate increase of GM content in the receptor field of 7%. This indicates that variations in the size of the donor field have a smaller influence on GM content than variations in the size of the receptor field.

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Identification and Registration of Maize Genotypes with the Use of Molecular Markers

Cited by 5 publications

References 8 publications

Molecular diversity and microsatellite polymorphism of modern maize hybrids

Molecular diversity and microsatellite polymorphism of modern maize hybrids

Genetic purity certificate in seeds of hybrid maize using molecular markers

Assessment of the influence of field size on maize gene flow using SSR analysis

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