Identification and quantitative analysis of β-sitosterol oxides in vegetable oils by capillary gas chromatography–mass spectrometry

Zhang, Xin; Julien-David, Diane; Miesch, Michel; Geoffroy, Philippe; Raul, Françis; Roussi, Stamatiki; Aoudé-Werner, Dalal; Marchioni, Eric

doi:10.1016/j.steroids.2005.06.004

Cited by 55 publications

(46 citation statements)

References 39 publications

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“…33. They performed a genomewide scan of the same family data by treating the hoarding score as a quantitative trait.…”

Section: Discussionmentioning

confidence: 99%

“…Normality is generally assumed for analyzing quantitative traits. In this data set, the hoarding score used in Zhang et al (33) came from two yes-no questions, and the normality is clearly a concern. It is important that we confirmed the earlier results by the new model and a more rigorous approach.…”

Section: Discussionmentioning

confidence: 99%

“…They found evidence in support of a recessive mode of transmission for the hoarding symptom dimension in families with two affected siblings with Gilles de la Tourette syndrome (GTS) (32). Zhang et al (33) performed linkage study of hoarding in the sibling pairs in the same study sample that we used here. They found strong evidence of linkage to three different regions by using a numerical factor scale from two hoarding items as a quantitative trait.…”

Section: Application On Obsessive-compulsive Disorder (Ocd)mentioning

confidence: 99%

“…No information has been collected to perform a justifiable ascertainment adjustment for hoarding, which was a limitation of this study, as it was for ref. 33.…”

Section: Application On Obsessive-compulsive Disorder (Ocd)mentioning

confidence: 99%

“…Parametric and nonparametric analyses were done with GENE-HUNTER and ALLEGRO. For parametric analysis, disease allele frequency was set at 0.3, and the penetrances were set at 0.125, 0.575, and 0.75 (33).…”

Section: Application On Obsessive-compulsive Disorder (Ocd)mentioning

confidence: 99%

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Linkage analysis of ordinal traits for pedigree data

Feng

Leckman

Zhang

2004

Proc. Natl. Acad. Sci. U.S.A.

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Linkage analysis is used routinely to map genes for human diseases and conditions. However, the existing linkage-analysis methods require that the diseases or conditions either be dichotomized or measured by a quantitative trait, such as blood pressure for hypertension. In the latter case, normality is generally assumed for the trait. However, many diseases and conditions, such as cancer and mental and behavioral conditions, are rated on ordinal scales. The objective of this study was to establish a framework to conduct linkage analysis for ordinal traits. We propose a latent-variable, proportional-odds logistic model that relates inheritance patterns to the distribution of the ordinal trait. We use the likelihood-ratio test for testing evidence of linkage. By means of simulation studies, we find that the power of our proposed model is substantially higher than that of the binary-trait-based linkage analysis and that our test statistic is robust with regard to certain parameter misspecifications. By using our proposed method, we performed a genome scan of the hoarding phenotype in a data set with 53 nuclear families, which were collected by the Tourette Syndrome Association International Consortium for Genetics (TSAICG). Standard linkage scans using hoarding as a dichotomous trait were also performed by using GENEHUNTER and ALLEGRO. Both GENEHUNTER and ALLEGRO failed to reveal any marker significantly linked to the binary hoarding phenotypes. However, our method identified three markers at 4q34-35 (P ‫؍‬ 0.0009), 5q35.2-35.3 (P ‫؍‬ 0.0001), and 17q25 (P ‫؍‬ 0.0005) that manifest significant allele sharing.

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“…33. They performed a genomewide scan of the same family data by treating the hoarding score as a quantitative trait.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Application On Obsessive-compulsive Disorder (Ocd)mentioning

confidence: 99%

“…No information has been collected to perform a justifiable ascertainment adjustment for hoarding, which was a limitation of this study, as it was for ref. 33.…”

Section: Application On Obsessive-compulsive Disorder (Ocd)mentioning

confidence: 99%

Section: Application On Obsessive-compulsive Disorder (Ocd)mentioning

confidence: 99%

See 3 more Smart Citations

Linkage analysis of ordinal traits for pedigree data

Feng

Leckman

Zhang

2004

Proc. Natl. Acad. Sci. U.S.A.

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Stabilization of phytosterols in rapeseed oil by natural antioxidants during heating

Kmiecik

Korczak

Rudzińska

et al. 2009

Euro J Lipid Sci & Tech

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Antioxidants are substances that can reduce negative changes in fat. Many antioxidants are very effective during storage, but during heating they lose their properties. It is very important to find antioxidants that will be stable at high temperatures and protect fat throughout the entire frying process. The aim of this study was to estimate the effect of natural and synthetic antioxidants on changes in phytosterols of rapeseed oil during heating. Oil with antioxidants was heated at 180 7C for 4 h in a Rancimat ® and in an Oxidograph ® . Ethanol extract of rosemary, ethanol extract of green tea, and BHT were used in the study. The contents of phytosterols (sitosterol, campesterol, avenasterol, brassicasterol, stigmasterol) and oxyphytosterols (7a-and 7b-hydroxysterol, 5a,6a-and 5b,6b-epoxysterol, 7-ketosterol and triols) were estimated by gas chromatography. In all samples with antioxidants, a lower decrease of phytosterols and a lower increase of total oxyphytosterols were observed in comparison with the control sample (without antioxidant). The antioxidant effect depends on the type of the antioxidant and the heating conditions. The best results were observed in samples with natural antioxidants. BHT was a substance that protected phytosterols as well, but not as effectively as the other antioxidants.

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Bleaching augments lipid peroxidation products in pistachio oil and its cytotoxicity

Racicot

Craven

Chen

2012

Euro J Lipid Sci & Tech

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Pistachio consumption is associated with reductions in serum cholesterol and oxidative stress due to their constituents of unsaturated fats, phytosterols, fiber, and antioxidants. Bleaching has been applied to whiten nut shells for antifungal and cosmetic purposes. However, the impact of bleaching on nutritional quality and safety of pistachios remains to be examined. In this study, we investigated whether bleaching would increase malondialdehyde (MDA) or 7‐keto‐sitosterol and decrease phytosterols in pistachio oil, as well as cause cytotoxicity of modeled Hepa1c1c7 cells. Bleaching increased MDA by more than 32% from 0.23 µg/g in raw oil, with the largest increase noted with the bleach containing H2O2 and Fe2+ (P ≤ 0.05). Bleached pistachio oil had larger than 12.6% decrease in β‐sitosterol and total phytosterols as compared to the raw oil (P ≤ 0.05). Bleaching with Fe2+ significantly increase 7‐keto‐sitosterol compared to bleaching alone. Hepatic cell viability was decreased the most by the oil of the pistachios treated with bleach containing Fe2+ (P ≤ 0.05), and lactate dehydrogenase activity in medium was elevated by >18‐folds (P ≤ 0.05). Compared to natural pistachios, the bleaching treatment had detrimental effects on nutritional quality and expected health benefits of pistachios by increasing lipid peroxidation, decreasing phytosterol content, and causing cytotoxicity.Practical applications: Bleaching has been applied to whiten the nut shell for antifungal and cosmetic purposes. However, the results of this study indicate that bleaching treatment has a detrimental impact on nutritional quality and expected health benefits of pistachios. Particularly, treatment with a bleach formula with hydrogen peroxide and transit metals increases formation of lipid peroxidation products and decreases phytosterol content. The resulting pistachio oil causes cell toxicity. Thus, bleaching practice for whitening pistachios is strongly discouraged.

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Identification and quantitative analysis of β-sitosterol oxides in vegetable oils by capillary gas chromatography–mass spectrometry

Cited by 55 publications

References 39 publications

Linkage analysis of ordinal traits for pedigree data

Linkage analysis of ordinal traits for pedigree data

Stabilization of phytosterols in rapeseed oil by natural antioxidants during heating

Bleaching augments lipid peroxidation products in pistachio oil and its cytotoxicity

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