Identification and quantitation of platelet-associated fibronectin antigen.

Plow, Edward F.; Birdwell, Charles R.; Ginsberg, MH

doi:10.1172/jci109334

Cited by 92 publications

(33 citation statements)

References 15 publications

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“…Platelets were obtained from members of four unrelated thrombasthenic kindreds. Each kindred has been previously reported (10)(11)(12) (13,14) and were not reduced, confirming previous studies (15). Two of the kindreds showed markedly reduced levels of GP-III and Ilb as judged by lactoperoxidase catalyzed iodination and SDS-PAGE (16).…”

Section: Introductionsupporting

confidence: 90%

Reduced surface expression and binding of fibronectin by thrombin-stimulated thrombasthenic platelets.

Ginsberg¹,

Forsyth²,

Lightsey³

et al. 1983

J. Clin. Invest.

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163

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Section: Introductionsupporting

confidence: 90%

Reduced surface expression and binding of fibronectin by thrombin-stimulated thrombasthenic platelets.

Ginsberg¹,

Forsyth²,

Lightsey³

et al. 1983

J. Clin. Invest.

Self Cite

163

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“…In addition, platelets release a small amount of Fn when stimulated with thrombin or collagen (Plow et al 1979). An increase of plasma Fn levels in poorly controlled diabetic patients was reported in the literature previously, and elevated plasma Fn levels could be due to enhanced vessel wall production or due to increased release from platelets (Schwarz and Schernthaner 1982).…”

Section: Resultsmentioning

confidence: 99%

The effect of glycemic control on plasma soluble fibrin monomer complexes and fibronectin in diabetic patients.

Tada

Takeuchi

Takagi

et al. 1987

Tohoku J. Exp. Med.

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Med., 1987, 153 (2), 145 -150 We have shown previously that increased concentrations of plasma soluble fibrin monomer complexes (SFMC) and elevated fibronectin (Fn) levels are closely related to the development of diabetic microangiopathy. The purpose of the present study was to explore whether or not changes in plasma glucose levels could have an effect on these protein constituents. Plasma glucose levels of 25 uncontrolled diabetic patients were brought under control with insulin and serial measurements of SFMC and Fn were made over a period of 4 weeks. Glucose values fell from an average of 312 mg 100 ml to 160 mg 100 ml. Ten patients with macroproteinuria (i.e.>_ 0.5 g/24 hr) showed initially elevated plasma SFMC and Fn concentrations. These levels fell significantly over the 4 week observation period : from 13.6 mg/ 100 ml to 9.4 mg 100 ml for SFMC and from 38.4 mg 100 ml to 34.5 mg 100 ml for Fn. The remaining 15 patients had nearly normal levels of both SFMC (7.9 mg/ 100 ml) and Fn (31.1 mg 100 ml) and glycemic control brought no further reduction. The data indicated that a) elevated SFMC and Fn levels are indeed associated with diabetic microangiopathy, especially in the presence of macroproteinuria ; and b) adequate glycemic control is capable of normalizing the plasma concentration of these constituents.soluble fibrin monomer complexes ; fibronectin ; intravascular thrombin generation ; glycemic control It is well known that plasma soluble fibrin monomer complexes (SFMC) consist of the fibrin monomer, fibrinogen and fibrin degradation products. Fibrin monomer production essentially requires thrombin for the partial proteolysis of fibrinogen. In addition, the levels of SFMC closely correlates to that of /3-thromboglobulin (Iioka 1984). Hence, the increase in the levels of SFMC is an indicator of the intracapillary thrombin activation.Our recent studies revealed that plasma levels of SFMC and Fn in diabetics with advanced diabetic microangiopathy, especially in the presence of macroproteinuria, were higher than those seen in patients without diabetic microangiopathy.Moreover, plasma Fn level

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“…The major factors for cell attachment derived from mammalian platelets are FN and VN (24,25,32). This fact indicates that the cell attachment activity of TSP prepared from thrombocytes may be closely related with FN and/or VN.…”

Section: Resultsmentioning

confidence: 99%

“…In addition, platelets are known to contain adhesive glycoproteins such as fibrinogen (22), fibronectin (FN) (24,32) and vitronectin (VN) (25), which are also released by thrombin stimulation.…”

mentioning

confidence: 99%

Identification of Fibronectin in Chicken Thrombocytes.

Horiuchi

Hayashi

Matsuda

et al. 1992

Cell Struct. Funct.

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ABSTRACT. Cell attachment and spreading of chicken embryo fibroblasts and three mammaliancell lines were promoted by using thrombocyte secretion products (TSP) prepared from chicken thrombocytes which are analogous to mammalian platelets. The cell attachment activity following TSP treatment was interfered with by the addition of a synthetic peptide containing the RGDsequence from the cell attachment region of fibronectin (FN). Cell attachment on TSP-coated plates was inhibited by the addition of anti-chicken FN rabbit antiserum, but not by anti-chicken vitronectin (VN) rabbit antiserum. By immunoblotting, immunofluorescent test and sensitive enzymelinked immunosorbent assay using an anti-chicken FNantiserum, the presence of FNin thrombocytes and the release of FNfrom the cells were demonstrated. The release of FNfrom thrombocytes was partially induced by the in vitro cultivation of the cells and was further promoted by treatment of the cells with thrombin.

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Identification and quantitation of platelet-associated fibronectin antigen.

Cited by 92 publications

References 15 publications

Reduced surface expression and binding of fibronectin by thrombin-stimulated thrombasthenic platelets.

Reduced surface expression and binding of fibronectin by thrombin-stimulated thrombasthenic platelets.

The effect of glycemic control on plasma soluble fibrin monomer complexes and fibronectin in diabetic patients.

Identification of Fibronectin in Chicken Thrombocytes.

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