Identification and quantitation of indole-3-acetic and abscisic acids in the cambial region of Quercus robur by combined gas chromatography-mass spectrometry

Browning, G.; Wignall, T. A.

doi:10.1093/treephys/3.3.235

Cited by 17 publications

(4 citation statements)

References 21 publications

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“…Frozen samples were lyophilized and stored at −70 °C until extraction. The level of endogenous ABA was quantified following the method of Browning and Wignall (1987), Qi et al. (1998), and Kamboj et al.…”

Section: Methodsmentioning

confidence: 99%

“…Frozen samples were lyophilized and stored at )70°C until extraction. The level of endogenous ABA was quantified following the method of Browning and Wignall (1987), Qi et al (1998), and Kamboj et al (1999). Plant samples were extracted with 30 ml of extraction solution containing 95 % isopropanol, 5 % glacial acetic acid, and 100 ng of [(±)-3,5,5,7,7,7-d 6 ]-ABA.…”

Section: Quantification Of Endogenous Aba and Ja Levelsmentioning

confidence: 99%

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Jasmonic Acid Differentially Affects Growth, Ion Uptake and Abscisic Acid Concentration in Salt‐tolerant and Salt‐sensitive Rice Cultivars

Kang¹,

Seo²,

Lee³

et al. 2005

J Agronomy Crop Science

220

102

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Phytohormones play critical roles in regulating plant responses to stress. Here, we investigated the effects of salt stress and stress recovery by applying jasmonate to the two different rice (Oryza sativa L.) cultivars Dongjinchalbyeo (DJC, salt-tolerant) and Dongjinbyeo (DJ, salt-sensitive). Salt stress remarkably decreased the root length of plants even at low NaCl concentration (20 mm). Salt stress led to a sharp increase in the concentrations of abscisic acid (ABA) in 20 and 40 mm NaCl, when compared with the control values. The concentrations of ABA in the salt-tolerant cultivar DJC plants progressively increased with increasing NaCl levels, whereas in the salt-sensitive cultivar DJ, they sharply decreased in all three parts of rice plants at 80 mm NaCl treatment. The decrease of jasmonic acid (JA) concentrations in salt-tolerant cultivar DJC was lesser than in the saltsensitive cultivar DJ plants in the shoot. Post-application in the stressed plants with 30 lm JA at 24 and 48 h after NaCl treatment, recovered salt inhibition on dry mass production more effectively than application of JA at 48 and 24 h before salt stress, and during salt stress simultaneously. The uptake of Na decreased especially in the salt-sensitive cultivar DJ plants, whereas there was an increase in Ca and Mg levels and slight increase of K by JA application. Leaf water potential, leaf photosynthetic rate, and maximum quantum yield of photosystem II (PSII) also remarkably recovered when 30 lm JA was applied 24 h after the salt stress compared with the 40 mm NaCl-treated plants. These results clearly indicate that post-application with exogenous JA can ameliorate salt-stressed rice seedlings, especially the salt-sensitive cultivar rather than the salt-tolerant cultivar. This may change the balance of other endogenous plant hormones.Key words: abscisic acid -ion uptake -jasmonic acid -leaf photosynthesis -leaf water potential -maximum quantum yield of PSII (Fv/Fm)

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Section: Methodsmentioning

confidence: 99%

Section: Quantification Of Endogenous Aba and Ja Levelsmentioning

confidence: 99%

Jasmonic Acid Differentially Affects Growth, Ion Uptake and Abscisic Acid Concentration in Salt‐tolerant and Salt‐sensitive Rice Cultivars

Kang¹,

Seo²,

Lee³

et al. 2005

J Agronomy Crop Science

220

102

View full text Add to dashboard Cite

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“…The level of endogenous ABA was quantified by gas chromatography-mass spectrometry (GC-MS) as described previously [22][23][24][25][26]. Plants were immediately frozen in liquid nitrogen.…”

Section: Measurement Of Endogenous Aba By Gas Chromatography-mass Spementioning

confidence: 99%

A vacuole localized β-glucosidase contributes to drought tolerance in Arabidopsis

et al. 2011

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The phytohormone abscisic acid (ABA) plays a critical role in plant growth, development, and adaptation to various stress conditions. The cellular ABA level is constantly adjusted to respond to changing physiological and environmental conditions. To date, the mechanisms for fine-tuning ABA levels remain elusive. Here, we report that BGLU10, a member of a multigene family of β-glucosidases, contributes to drought tolerance in Arabidopsis. The T-DNA insertion mutant bglu10 exhibited a droughtsensitive phenotype, characterized by an increased rate of water loss, and lower leaf temperature, β-glucosidase activity, ABA content, and expressions of ABA-and drought-responsive genes under drought stress. In contrast, lines overexpressing BGLU10 showed greater drought resistance than that of the wild-type, as shown by decreased water loss via transpiration, higher β-glucosidase activity, ABA level, and expressions of ABA-and stress-responsive genes under drought stress. Transient expression of BGLU10::GFP and γ-TIP1::RFP in mesophyll cell protoplasts showed that the BGLU10 enzyme protein was localized to the vacuole. Meanwhile, BGLU10 was expressed in various organs, and was induced by several abiotic stresses, suggesting that BGLU10 may be involved in a variety of stress responses, and that hydrolysis of ABA-GE produces free ABA in the plant stress response.Arabidopsis, β-glucosidase, BGLU10, ABA, drought Citation: Wang P T, Liu H, Hua H J, et al. A vacuole localized β-glucosidase contributes to drought tolerance in Arabidopsis.

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“…The ABA content was analyzed using the method of Browning and Wignall (1987), Qi et al (1998), and Kamboj et al (1999) with modifications. The powdered seeds were extracted with 30 of solution containing 95% isopropanol and 5% glacial acetic acid, and 50 ng of [(±)-3,5,5,7,7,7-d6]-ABA standard was added into extracts.…”

Section: Analysis Of Endogenous Abscisic Acidmentioning

confidence: 99%

Effect of light on endogenous levels of gibberellin and abscisic acid in seed germination of photoblastic weedy rice (Oryza sativa L.)

Kim

Hwang

Lee

et al. 2009

J. Crop Sci. Biotechnol.

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The effect of red (R) and R/far-red (FR) lights on endogenous gibberellin (GA) and abscisic acid (ABA) content was first investigated during the germination of photoblastic black-hulled weedy rice (PBWR) seeds. The R light-treated PBWR seeds germinated after 36-48 h and germination was increased to 63% at 72 h. However, the FR light-treated seeds after R light treatment, suppressed the R light effect showing only 11% germination even at 72 h after the light treatment. The PBWR seed treated with R light rapidly increased the endogenous level of GA1 to about 200 times at 12 h before seed germination as compared with R/FR (control) which suppressed the effect of R light. The contents of other GAs like GA12, GA53, GA19, GA20, and GA8 were not affected by the R light irradiation. These results showed that the major biosynthetic pathway of GAs in PBWR seeds is the early 13-hydroxylation pathway leading to GA1, which was suggested to be physiologically active in the PBWR seed germination. The decrease in the level of ABA in the R light-treated seeds was greater than the R/FR light-treated seeds, indicating that the balance of endogenous GA1 and ABA is responsible for the induction of germination in the PBWR seed.

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Identification and quantitation of indole-3-acetic and abscisic acids in the cambial region of Quercus robur by combined gas chromatography-mass spectrometry

Cited by 17 publications

References 21 publications

Jasmonic Acid Differentially Affects Growth, Ion Uptake and Abscisic Acid Concentration in Salt‐tolerant and Salt‐sensitive Rice Cultivars

Jasmonic Acid Differentially Affects Growth, Ion Uptake and Abscisic Acid Concentration in Salt‐tolerant and Salt‐sensitive Rice Cultivars

A vacuole localized β-glucosidase contributes to drought tolerance in Arabidopsis

Effect of light on endogenous levels of gibberellin and abscisic acid in seed germination of photoblastic weedy rice (Oryza sativa L.)

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