Identification and Quantification of S-Sulfenylation Proteome of Mycobacterium tuberculosis under Oxidative Stress

Lu, Yuanyao; Chen, Hongtong; Wang, Penghe; Pang, Jing; Lü, Xi; Li, Guoqing; Hu, Xinxin; Wang, Xiukun; Yang, Xinyi; Li, Congran; Lu, Yu; You, Xuefu

doi:10.1128/spectrum.03386-22

Cited by 2 publications

(4 citation statements)

References 42 publications

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“…The phagocytosis of MTB by macrophages also triggers a respiratory burst, producing a series of anti‐MTB effector molecules, such as reactive oxygen intermediates and reactive nitrogen intermediates (RNIs) 41 . H 2 O 2 , an intermediate of reactive oxygen intermediates, is one of the earliest molecules recognized to mediate MTB clearance 42,43 . Last, different subtypes of macrophages play important roles in the clearance of MTB 44,45 .…”

Section: Immunologic Mechanisms Of Mtb–host Interactionsmentioning

confidence: 99%

Mycobacterium tuberculosis: immune response, biomarkers, and therapeutic intervention

Zhuang,

Yang,

et al. 2024

MedComm

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Although tuberculosis (TB) is an infectious disease, the progression of the disease following Mycobacterium tuberculosis (MTB) infection is closely associated with the host's immune response. In this review, a comprehensive analysis of TB prevention, diagnosis, and treatment was conducted from an immunological perspective. First, we delved into the host's immune response mechanisms against MTB infection as well as the immune evasion mechanisms of the bacteria. Addressing the challenges currently faced in TB diagnosis and treatment, we also emphasized the importance of protein, genetic, and immunological biomarkers, aiming to provide new insights for early and personalized diagnosis and treatment of TB. Building upon this foundation, we further discussed intervention strategies involving chemical and immunological treatments for the increasingly critical issue of drug‐resistant TB and other forms of TB. Finally, we summarized TB prevention, diagnosis, and treatment challenges and put forward future perspectives. Overall, these findings provide valuable insights into the immunological aspects of TB and offer new directions toward achieving the WHO's goal of eradicating TB by 2035.

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Section: Immunologic Mechanisms Of Mtb–host Interactionsmentioning

confidence: 99%

Mycobacterium tuberculosis: immune response, biomarkers, and therapeutic intervention

Zhuang,

Yang,

et al. 2024

MedComm

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“…Mycobacteria can adapt to stress conditions, including oxidative stress, nutrient starvation, pH change, and temperature, which elucidate its evolutional adaptation by a refined network of molecular mechanisms [ 10 – 13 ]. The production of reactive oxygen species (ROS) and the acidification of phagosomes are the critical mechanisms that macrophages employ to kill internalized pathogens during infections [ 14 – 17 ].…”

Section: Introductionmentioning

confidence: 99%

“…For example, Lu et al investigated the proteomic profiling of Mycobacterium tuberculosis (Mtb) under oxidative stress, focusing on total cysteine thiols modification and S-sulfenylation modification [ 10 ]. They identified the differential expression of numerous proteins at total cysteine modification and S-sulfenylation modification levels under hydrogen peroxide exposure [ 10 ]. These cysteine-modified proteins were associated with oxidation–reduction, fatty acid synthesis, cell wall remodeling, and protein repair [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

“…They identified the differential expression of numerous proteins at total cysteine modification and S-sulfenylation modification levels under hydrogen peroxide exposure [ 10 ]. These cysteine-modified proteins were associated with oxidation–reduction, fatty acid synthesis, cell wall remodeling, and protein repair [ 10 ]. Recently, Yimcharoen et al investigated the transcriptional response of drug susceptible and resistant Mtb strains after isoniazid exposure under stress conditions that mimic the host environment [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

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Prominent transcriptomic changes in Mycobacterium intracellulare under acidic and oxidative stress

Park,

Kim,

Shin

et al. 2024

BMC Genomics

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Background Mycobacterium avium complex (MAC), including Mycobacterium intracellulare is a member of slow-growing mycobacteria and contributes to a substantial proportion of nontuberculous mycobacterial lung disease in humans affecting immunocompromised and elderly populations. Adaptation of pathogens in hostile environments is crucial in establishing infection and persistence within the host. However, the sophisticated cellular and molecular mechanisms of stress response in M. intracellulare still need to be fully explored. We aimed to elucidate the transcriptional response of M. intracellulare under acidic and oxidative stress conditions. Results At the transcriptome level, 80 genes were shown [FC] ≥ 2.0 and p < 0.05 under oxidative stress with 10 mM hydrogen peroxide. Specifically, 77 genes were upregulated, while 3 genes were downregulated. In functional analysis, oxidative stress conditions activate DNA replication, nucleotide excision repair, mismatch repair, homologous recombination, and tuberculosis pathways. Additionally, our results demonstrate that DNA replication and repair system genes, such as dnaB, dinG, urvB, uvrD2, and recA, are indispensable for resistance to oxidative stress. On the contrary, 878 genes were shown [FC] ≥ 2.0 and p < 0.05 under acidic stress with pH 4.5. Among these genes, 339 were upregulated, while 539 were downregulated. Functional analysis highlighted nitrogen and sulfur metabolism pathways as the primary responses to acidic stress. Our findings provide evidence of the critical role played by nitrogen and sulfur metabolism genes in the response to acidic stress, including narGHIJ, nirBD, narU, narK3, cysND, cysC, cysH, ferredoxin 1 and 2, and formate dehydrogenase. Conclusion Our results suggest the activation of several pathways potentially critical for the survival of M. intracellulare under a hostile microenvironment within the host. This study indicates the importance of stress responses in M. intracellulare infection and identifies promising therapeutic targets.

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Identification and Quantification of S-Sulfenylation Proteome of Mycobacterium tuberculosis under Oxidative Stress

Abstract: With the continuous spread of drug-resistant tuberculosis, there is an urgent need for new antituberculosis drugs with new mechanisms. The ability of Mtb to resist oxidative stress is extremely important for maintaining redox homeostasis and survival in the host.

Cited by 2 publications

References 42 publications

Mycobacterium tuberculosis: immune response, biomarkers, and therapeutic intervention

Mycobacterium tuberculosis: immune response, biomarkers, and therapeutic intervention

Prominent transcriptomic changes in Mycobacterium intracellulare under acidic and oxidative stress

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