Identification and Quantification of Phenolic Compounds from the Forage Legume Sainfoin (Onobrychis viciifolia)

Abstract

Phenolic compounds of sainfoin ( Onobrychis viciifolia ) variety Cotswold Common are assumed to contribute to its nutritive value and bioactive properties. A purified acetone/water extract was separated by Sephadex LH-20 gel chromatography. Sixty-three phenolic and other aromatic compounds were identified by means of chemical, chromatographic, and spectroscopic methods. Reverse phase HPLC with diode array and chemical reaction detection was used to investigate the phenolic composition of different plant organs. All plant parts showed specific phenolic profiles. Moreover, there were considerable variations in the phenolic content among individual plants of the same variety. The three most abundant phenolic compounds were found to be arbutin [predominant in petiols, 17.7 mg/g of dry weight (DW)], rutin (predominant in leaves, 19.9 mg/g of DW), and catechin (predominant flavanol in petiols, 3.5 mg/g of DW). The present study reveals that the phenolic profile of sainfoin is even more complex than hitherto assumed.

Identification of phenolic constituents of Cytisus multiflorus

“…Moreover, the fragmentation pathway of the base peak at m/z 449 corroborated the hypothesis of a luteolin-C-glucoside derivative (Ioset et al, 2007) and the loss of 132 Da from the product ion at m/z 449 confirmed the presence of a pentose unit with a O-linkage (Cuyckens & Claeys, 2004;Han et al, 2008;Regos, Urbanella, & Treutter, 2009;Ye, Yan, & Guo, 2005). Overall, the above results allowed the detection, for the first time, of two isomers of 2 00 -O-pentosyl-C-hexosyl-luteolin derivatives in Cytisus.…”

Investigation of sainfoin (Onobrychis viciifolia) cultivar differences on nitrogen balance and fecal egg count in artificially infected lambs1

“…In fact, in vitro studies with sainfoin extracts by Barrau et al (2005), who used the larval migration inhibition assay, showed that other polyphenols, such as rutin, nicotifl orin, and narcissin (all fl avonol glycosides), also inhibited larval migration. Furthermore, Regos et al (2009) found that rutin and catechin were of the most abundant phenolic compounds in sainfoin, and this lends further credence to the above argument.…”

Promising options for improving performance and proanthocyanidins of the forage legume sainfoin (Onobrychis viciifolia Scop.)

“…As expected for an outbreeding species, large variability in PA content and 263 composition was also observed between individual plants within accessions. PA content and 264 composition is also known to vary considerable within the plant, with young leaves having highest 265 and stems having lowest concentrations (Regos et al 2009). In addition, sainfoin leaves appeared to 266 better meet the PA composition required for optimal health and nutritional benefits.…”