Identification and quantification of nine compounds in Fangwen Jiuwei decoction by liquid chromatography–mass spectrometry

To improve the effectiveness of marketed drugs related to active ingredients, it is necessary to designate a more unified quality evaluation standard. Taking Nvjin Pills as an example, this study reported the development of a novel principle of analysis in traditional Chinese medicine. The core of the experiment is to prepare three batches of traditional Chinese medicine reference drugs by high‐quality Chinese materia medica. The active ingredients identified in the herbal formula including glycyrrhizic acid, cinnamaldehyde, paeonol, baicalin, hesperidin, paeoniflorin, and ferulic acid were analyzed in traditional Chinese medicine reference drugs by the high‐performance liquid chromatography method combined with wavelength switching. The simple prediction results of network pharmacological analysis verified the feasibility and reliability of the established quantitative analysis method for seven target‐focused compounds in Nvjin Pills, which were recommended as candidate indicators for quality evaluation ultimately. Using the seven target‐focused compounds as the scientific ruler, quality grade specifications of Nvjin Pills were proposed by comprehensive analysis. Accordingly, 16, 47, and 13 batches of samples were primarily graded as first grade, second grade, and unqualified grade, respectively. This study will provide a chemical basis for quality control of Nvjin Pills, which is necessary for the production process of pharmaceutical development.

Section: Explore the Anti-polycystic Ovary Syndrome Activity Of Targe...mentioning

confidence: 99%

Quality grade evaluation of Nvjin Pills based on traditional Chinese medicine reference drug and network pharmacology of target‐focused compounds

Lin,

Liu,

Zhang

et al. 2023

“…CKD incidence is increasing globally and has become a major public health burden [4,5]. Current treatments for CKD are limited; thus, many patients seek alternative therapies, such as traditional Chinese medicine (TCM) [6,7]. Several basic and clinical studies have confirmed that TCM protects kidney function and delays CKD progression [8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of multiple constituents, serum composition, and tissue distribution of Qingshen granule using ultra‐high performance liquid chromatography‐quadrupole‐orbitrap high‐resolution mass spectrometry

Dai

Chen

et al. 2023

Qingshen granule, composed of 14 herbal drugs, is primarily used as the assistant therapy for chronic kidney disease. Qingshen granule chemical composition was complex, but its chemical constituents and the pharmacodynamic material basis remain unreported. Ultra-high-performance liquid chromatography (UHPLC)-quadrupole-orbitrap high-resolution mass spectrometry was applied to recognize the chemical constituents of Qingshen granule. The analysis was performed using the ACQUITY UHPLC BEH C18 column (2.1 × 50 mm, 1.7 μm) with acetonitrile-0.1% formic acid as the mobile phase for gradient elution. The data were collected using heated electrospray ionization in positive and negative ion modes. This study successfully applied the UPHLC-quadrupole-orbitrap high-resolution mass spectrometry technique with the Compound Discoverer 3.3 platform to analyze Qingshen granule chemical composition. A total of 127 and 42 chemical components were identified in Qingshen granule in vitro and in vivo, respectively. In the tissue distribution of Qingshen granule, 9, 10, 11, 10, and 18 prototype components were detected in the heart, liver, spleen, lungs, and kidneys, respectively. Qingshen granule chemical constituents were characterized rapidly for the first time in this study, laying a foundation for further research on the substance basis and quality control of Qingshen granule in treating chronic kidney disease.

“…The working schematic diagram is shown in Figure 1. Though it seemed a routine strategy in the TCMP study [15, 16], special improvement and innovation were conducted in this study according to the concrete circumstances of MTZSFG. Liquid–liquid extraction was introduced into the sample pretreatment process to obtain fractions of different polarities, which were analyzed separately in order to overcome the complexity of components in MTZSFG formulation.…”

Section: Introductionmentioning

confidence: 99%

Chemical profiling and mechanistic studies of Zhi‐Shang‐Feng granules against influenza virus by high‐performance liquid chromatography coupled with Q exactive focus hybrid quadrupole orbitrap high‐resolution mass spectrometry in combination with network pharmacology analysis

Xie,

Luo,

et al. 2023

Zhi‐Shang‐Feng Granules are used in the clinical treatment of influenza to relieve headaches, chills and fever, bronchitis, nasal congestion, neuralgia and other symptoms. To decipher the components responsible for therapeutic effects of Zhi‐Shang‐Feng g ranules against influenza virus, an analytical method based on high‐performance liquid chromatography coupled with Q exactive focus hybrid quadrupole orbitrap high resolution mass spectrometry was developed and the chemical profile of Zhi‐Shang‐Feng granules was characterized. Then, the identified components were used to conduct network pharmacological analysis and determine the potential mechanism of Zhi‐Shang‐Feng Granules. As a result, 177 compounds were putatively identified through comprehensive analysis by liquid chromatography coupled with high‐resolution mass spectrometry, of which 23 compounds were unambiguously confirmed with reference standards. Components in Zhi‐Shang‐Feng Granules were found to specifically act on different enzymes, G‐protein‐coupled receptors, ion channels and transporters in the immune, endocrine, nervous, and circulatory systems. The potential mechanism was related to several biological processes, including cell growth and death, pattern recognition receptor signalling, signalling by interleukins, and lipid metabolism. The combination of chemical profile characterization and network construction provided useful insight into the overall chemical composition of Zhi‐Shang‐Feng granules and revealed their potential anti‐infection, anti‐inflammatory and immunoregulatory mechanisms against influenza virus infected disease.