Identification and Quantification of Methanogenic Archaea in Adult Chicken Ceca

Saengkerdsub, Suwat; Anderson, Robin C.; Wilkinson, Heather H.; Kim, Woo-Kyun; Nisbet, David J.; Ricke, Steven C.

doi:10.1128/aem.01931-06

Cited by 97 publications

(73 citation statements)

References 28 publications

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“…This finding is consistent with other studies where Methanobrevibacter strains accounted for the vast majority of methanogens in chicken ceca (Saengkerdsub et al, 2007), the lower termite Reticulitermes speratus (Shinzato et al, 1999) and the rumen Sharp et al, 1998;Tokura et al, 1999;Whitford et al, 2001;Irbis and Ushida, 2004;Skillman et al, 2004Skillman et al, , 2006Wright et al, 2004Wright et al, , 2008. Moreover, this finding supports our hypothesis that the genus Methanobrevibacter is the most dominant constituent of foregut fermentation systems.…”

Section: Discussionsupporting

confidence: 93%

“…The mean density of hoatzin crop methanogens (5.80 Â 10 9 cells per gram wet weight) from the present study was up to 20 000 times greater than methanogen densities found in chicken cecal samples, which were based on both most probable number enumeration and 16S rRNA copy numbers (Saengkerdsub et al, 2007). However, the mean density of methanogens in the hoatzin crop was in agreement with real-time PCR densities of methanogens from cattle from Canada using 16S rRNA (7.93 Â 10 4 to 1.62 Â 10 9 ; Hook et al, 2009), and cattle from Australia using the mcrA genes (9.8 Â 10 8 , Evans et al, 2009;1.34 Â 10 9 , Denman et al, 2007).…”

Section: Discussioncontrasting

confidence: 67%

“…Saengkerdsub et al (2007) examined the methanogenic archaea in adult chicken ceca, using the same methanogen-specific forward and reverse primers (Met86F and Met1340R), and grouped 420 clones into 11 different phylotypes, 10 of which were 99% similar to Methanobrevibacter woesei. In one other study , methanogen strains isolated from turkey and chicken feces appeared to belong to the genus Methanogenium (König, 1986).…”

Section: Discussionmentioning

confidence: 99%

“…However, methanogens have been reported from other avian species. Methanobrevibacter woesei was isolated from enrichments of goose feces Miller and Lin, 2002) and was also the predominant methanogen in chicken ceca (Saengkerdsub et al, 2007). Methanogenium-like strains also appear to have been reported from chicken and turkey feces, but no sequence data were reported (Kö nig, 1986;.…”

Section: Introductionmentioning

confidence: 99%

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Rumen-like methanogens identified from the crop of the folivorous South American bird, the hoatzin (Opisthocomus hoazin)

2009

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The hoatzin is the only known avian species with foregut fermentation. It is a primarily folivorous feeder and has a distended crop and lower/distal esophagus, which has evolved for the microbial fermentation of ingested feed. Crop samples collected from 10 individual animals from the Apure River area, Apure State, Venezuela were examined for the presence and density of methanogens using 16S rRNA gene clone libraries and real-time PCR prepared from pooled and individual PCR products. A total of 197 clones were examined, revealing 24 different methanogen 16S rRNA sequences, or phylotypes. Of the 24 unique phylotypes, 16 (171 of 197 clones) formed five unique clades within the genus Methanobrevibacter with the largest group of clones (118 clones) 98.7% similar to Methanobrevibacter ruminantium. The remaining eight phylotypes (26 clones) formed four unique clades that had only 94.0-96.7% identity to Methanosphaera stadtmanae. Based upon 98% sequence identity, we identified 17 of the 24 methanogen phylotypes from the hoatzin as possible new species and strains, with three phylotypes representing possible new genera (o94.5% sequence identity). Although none of the hoatzin methanogen phylotypes had 100% sequence identity to any other archaeal sequences in the GenBank database, the hoatzin crop methanogen sequences formed sister groups with known rumen methanogens. Mean population densities (numbers per gram wet weight) of methanogenic archaea, rumen bacteria and ciliate protozoa, estimated using real-time PCR, were 5.80 Â 10 9 , 7.93 Â 10 12 and 3.31 Â 10 5 , respectively. The crop microbial data presented here provide an excellent example of convergent evolution of foregut fermentation in the hoatzin, similar to that of ruminants.

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Section: Discussionsupporting

confidence: 93%

Section: Discussioncontrasting

confidence: 67%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Rumen-like methanogens identified from the crop of the folivorous South American bird, the hoatzin (Opisthocomus hoazin)

2009

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“…Methanobrevibacter ruminantium was the highest represented methanogen (64.5%) in the crop of the hoatzin, followed by a substantial representation of unassigned Methanobrevibacter species (21.3%) and unassigned Methanosphaera species (13.2%; Wright et al, 2009). In chickens, Methanobrevibacter woesei was found to be the most prevalent methanogen in the ceaca (96.7%; Saengkerdsub et al, 2007). This was the first report of M. woesei as the most prevalent methanogen in a GIT.…”

Section: Birdsmentioning

confidence: 78%

Diversity of gut methanogens in herbivorous animals

St-Pierre

Wright

2013

Animal

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The digestion of plant biomass by symbiotic microbial communities in the gut of herbivore hosts also results in the production of methane, a greenhouse gas that is released into the environment where it contributes to climate change. As methane is exclusively produced by methanogenic archaea, various research groups have devoted their efforts to investigate the population structure of symbiotic methanogens in the gut of herbivores. In this review, we summarized and compared currently available results from 16S rRNA gene clone library studies, which cover a broad range of hosts from ruminant livestock species to wild ruminants, camelids, marsupials, primates, birds and reptiles. Although gut methanogens are very diverse, they tend to be limited to specific phylogenetic groups. Overall, methanogens related to species of the genus Methanobrevibacter are the most highly represented archaea in the gut of herbivores. However, under certain conditions, archaea from more phylogenetically distant groups are the most prevalent, such as methanogens belonging to either the genus Methanosphaera, the order Methanomicrobiales or the Thermoplasmatales-Affiliated Lineage C Comparisons not only highlight the strong influence of host species and diet in the determination of the population structure of symbiotic methanogens, but also reveal other complex relationships, such as wide differences between breeds, as well as unexpected similarities between unrelated species. These observations strongly support the need for high throughput sequencing and metagenomic studies to gain further insight.

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Probiotics as Pathogen Control Agents for Organic Meat Production

Siragusa¹,

Ricke

2012

Organic Meat Production and Processing

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Identification and Quantification of Methanogenic Archaea in Adult Chicken Ceca

Cited by 97 publications

References 28 publications

Rumen-like methanogens identified from the crop of the folivorous South American bird, the hoatzin (Opisthocomus hoazin)

Rumen-like methanogens identified from the crop of the folivorous South American bird, the hoatzin (Opisthocomus hoazin)

Diversity of gut methanogens in herbivorous animals

Probiotics as Pathogen Control Agents for Organic Meat Production

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