Identification and quantification of major malonyl ginsenosides isolated fromPanax ginsengC.A. Meyer

Abstract

The root of Panax ginseng C.A. Meyer were extracted with 70% aqueous EtOH and the concentrates were partitioned into MeOH and H 2 O fractions using Diaion HP-20. The repeated SiO 2 or octadecyl SiO 2 column, and MPLC for the MeOH fraction led to isolation of four malonyl ginsenosides. The chemical structures of these compounds were determined as malonyl ginsenoside Rd (1) malonyl ginsenoside Rc (2) malonyl ginsenoside Rb2 (3) malonyl ginsenoside Rb1 (4) based on spectroscopic analyses including Nuclear magnetic resonance and HR-TOF/MS. The contents of malonyl ginsenoside Rb1 was highist as 5.44 mg/g of five years of ginseng. And malonyl ginsenoside Rd was lowest as 0.11 mg/g of six years of ginseng. Additionally, the malonyl ginsenoside Rd exhibited hepatoprotective effect against ethanol-induced hepatotoxicity in HepG2 cell line.

A Comparative Study on Processed Panax ginseng Products Using HR-MAS NMR-Based Metabolomics

“…A previous study on ginsenosides of processed ginseng products was conducted using UPLC-QTOF/MS-based metabolomics. Malonyl-ginsenoside Rb1, Rb2, Rc, ginsenoside Re, and Rg1 are the major constituents of WG, ginsenoside Rb2, Rc, Rd, Re, and Rg1 are the major constituents of TG, ginsenoside Rb1, Rb2, Rc, Rd, Re, and Rg1 are the major constituents of RG, and ginsenoside Rd, Rk1, Rg5, and Rg3 are the major constituents of BG [6,7]. Depending on the processing method, the amount of ginsenosides may be increased or decreased by chemical transformation [8].…”