Identification and quantification of gossypol in cotton by using packed micro-tips columns in combination with HPLC

Abstract

Self-packed micro-tip columns containing a C18-bonded silica stationary phase, based on the same principles as solid-phase extraction methods, were used to obtain gossypol and related sesquiterpenoid aldehyde-enriched fractions. The enriched metabolite fractions were then analyzed by optimized high-performance liquid chromatography (HPLC) with a C18 column (4.6 mm x 25 cm) eluted with the binary mobile phase acetonitrile-0.1% aqueous TFA solution (80:20). This method has proven to be highly reproducible. The precision and accuracy, as %RSD and %RME values, were determined to be less than 15% for the method. The minimum detection limit of gossypol was determined to be 10 ng (absolute gossypol). Absolute recovery was greater than 94% with a standard deviation of +/-3.68%. This is a simple, fast, and cost-effective method for isolation, identification, and quantification of gossypol and related secondary metabolites. Comparative analysis of gossypol content was performed on different parts of the cotton plant (seeds, stems and leaves) of two different cultivars of Gossypium hirsutum L. (Acala(1517-70) and OR19). The results indicate that the OR19 cv naturally contains higher gossypol levels than the Acala cv. It was also found that treatment of leaves with a Verticillium dahliae-derived elicitor induced production of deoxyhemigossypol rather than gossypol.

A comprehensive tool in recycling plant-waste of Gossypium barbadense L agricultural and industrial waste extracts containing Gossypin and Gossypol:Hepatoprotective, anti-inflammatory and antioxidant effects

“…The choice of the proper solvents; CHCL3 for gossypol, and EtOAc for gossypin may depend on the solubility of the phenolic nature of these components [33]. These findings are in agreement with Meyer et al (2004) [34] who indicated that the methanol extract is the best solvent to be used for the quantitative HPLC of these phenolics. The industrial waste showed to contain only gossypin and free from gossypol because this waste contain some seed as appear in (fig 1c) that findings are agreements with European Food Safety Authority [35]…”

Efficient Production of Gossypol from Hairy Root Cultures of Cotton (Gossypium hirsutum L.)

“…The gossypol content in the seed and other organs is closely related to the overall glandulosity and the genotype [12,64,65]. The total gossypol content of seeds of different genotypes varied from as low as 0.2 ± 0.05 mg/g DW to as high as 64 ± 1.5 mg/g DW [62,66]. One acre of cotton crop is estimated to yield 150 kg dry root bark, from which about 1.4 kg of pure gossypol can be recovered [67].…”

Pest trade-offs in technology: reduced damage by caterpillars in Bt cotton benefits aphids

“…The following elution gradient was applied: 0 min: 60 per cent B (40% A); 2 min: 60 per cent B; 20 min: 70 per cent B; 21 min: 100 per cent B; 25 min: 100 per cent B; 26 min: 60 per cent B; 35 min: 60 per cent B. Eluent A consisted of MilliQ-water, and eluent B consisted of acetonitrile. Eluent A was acidified with 0.1 per cent trifluoroacetic acid (greater than or equal to 99%, Riedel de Haën, Seelze Germany) to pH ¼ 2.5 (based on [22]). The flow rate of the mobile phase was 0.125 ml min -1 , and a 10 ml sample was injected.…”