Identification and quantification of anammox bacteria in eight nitrogen removal reactors

Hu, Baolan; Zheng, Ping; Tang, Chong–Jian; Chen, Jianwei; Biezen, Erwin van der; Zhang, Lei; Ni, Bing‐Jie; Jetten, Mike S. M.; Yan, Jia; Yu, Han‐Qing; Kartal, Boran

doi:10.1016/j.watres.2010.07.021

Cited by 169 publications

(76 citation statements)

References 37 publications

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“…As a result, Brocadia sinica could be enriched in the reactor. Besides, Hu et al (2010) found that ''Ca. Brocadia" was the abundant anammox species in wastewater treatment where organic compounds, ammonium, nitrite and nitrate were all present.…”

Section: Microbial Community Of the Sad Processmentioning

confidence: 99%

Performance and microbial community of simultaneous anammox and denitrification (SAD) process in a sequencing batch reactor

Qiang

et al. 2016

Bioresource Technology

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h i g h l i g h t sSimultaneous nitrogen and carbon removal was achieved in the SAD process. Contributions of anammox and denitrification to nitrogen removal were determined. Anammox and denitrifying bacteria dominated at low and high CODs, respectively. Brocadia sinica could grow with organic matter and tolerate high NO 3 À concentrations.a r t i c l e i n f o exhibited good diversity and abundance, but the diversity of anammox bacteria was less abundant. Brocadia sinica was able to grow in the presence of organic matter and tolerate high nitrite concentration. Anammox bacteria were predominant at low COD contents, while denitrifying bacteria dominated the microbial community at high COD contents. Anammox and denitrifying bacteria could coexist in one reactor to achieve the simultaneous carbon and nitrogen removal through the synergy of anammox and denitrification.

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Section: Microbial Community Of the Sad Processmentioning

confidence: 99%

Performance and microbial community of simultaneous anammox and denitrification (SAD) process in a sequencing batch reactor

Qiang

et al. 2016

Bioresource Technology

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“…qPCR assays were performed using SYBR Green I and conducted in FTC2000 fluorescence real-time PCR system (Canada). Primer pairs included Bacteria1055f (5′-ATGGCTGTCGTCAGCT-3′) and Bacteria1392r (5′-AC GGGCGGTGTGTAC-3′) for total bacterial 16S rRNA genes (Egli et al 2001), amoA-1 F (5′-GGGGTTTCTACTGGTG GT-3′) and amoA-2R (5′-CCCCTCKGSAAAGCCTTCTTC-3′) for AOB amoA genes (Pynaert et al 2003), and anammox-1 (5′-GGATTAGGCATGCAAGTC-3′) and anammox-2 (5′-TCTGTATTACCGCGGCT-3′) for anammox bacteria 16S rRNA genes (Tal et al 2006;Hu et al 2010). The 50-μL PCR reaction contained 25 μL of 2× PCR buffer, 1 μL of each primer (25 μM), 0.5 μL of SYBR Green I fluorescent dye, 2 μL of DNA template, and DEPC water.…”

Section: N Isotopic Tracing Experimentsmentioning

confidence: 99%

Nitrogen removal through different pathways in an aged refuse bioreactor treating mature landfill leachate

Xie

et al. 2012

Appl Microbiol Biotechnol

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In this study, an aged refuse bioreactor was constructed to remove nitrogen in a mature landfill leachate. The nitrogen removal efficiency and the microbial community composition in the bioreactor were investigated. The results showed that the aged refuse bioreactor removed more than 90 % of total nitrogen in the leachate under the nitrogen loading rate (NLR) of 0.74 g/kg (vs) day, and the total nitrogen removal rate decreased to 62.2 % when NLR increased up to 2.03 g/kg (vs) day. Quantitative polymerase chain reaction results showed that the average cell number of ammoniaoxidizing bacteria in the bioreactor was 1.58×10 8 cells/g, which accounted for 0.41 % of total bacteria. The number of anammox bacteria in the reactor was 1.09×10 8 cells/g, which accounted for 0.27 % of total bacteria. Isotopic 15 N tracing experiments showed that nearly 10 % of nitrogen was removed by anammox. High-throughout 454 pyrosequencing revealed that the predominant bacteria in the bioreactor were Proteobacteria, Chloroflexi, Actinobacteria, Bacteroidetes, and Gemmatimonadetes, including various nitrifiers and denitrifiers with diverse heterotrophic and autotrophic metabolic pathways, supporting that nitrogen was removed through different pathways in this aged refuse bioreactor.

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“…Li et al [39] reported successful operation of an Anammox reactor for more than 500 days with an abundance of Anammox bacteria of only 16%. Candidatus brocadia and Candidatus kuenenia, which were previously reported to be the predominant Anammox bacteria [43], were not detected in our Anammox-UASB reactor. Different sludge types and operating conditions can lead to shifts in the abundances of the three genera of Anammox bacteria.…”

Section: Discussionmentioning

confidence: 49%

Characterization of a Microbial Community in an Anammox Process Using Stored Anammox Sludge

Chen

Dai

Cao

et al. 2017

Water

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This study investigated a rapid start-up anaerobic ammonium oxidation (Anammox) process by inoculation with stored Anammox sludge and characterized the associated microbial communities. The Anammox process took only 43 days to start. A high nitrogen removal rate of 1.13 kg N m −3 d −1 and a nitrogen loading rate of 1.28 kg N m −3 d −1 were achieved. The ratio of ammonium removal to nitrite removal to nitrate production (1:1:0.2) was slightly lower than the theoretical value, which indicated nitrogen removal by denitrification in the reactor. Illumina high-throughput sequencing of sludge samples confirmed the co-existence of Anammox bacteria and denitrifying bacteria in the reactor and demonstrated that denitrifying bacteria play a role in nitrogen removal during the Anammox process. The dominant microbes in the reactor were Proteobacteria, Chlorobi, Chloroflexi, and Planctomycetes. However, only one species of Anammox bacteria, Candidatus jettenia, was identified and had an abundance of 4.92%. Our results illustrate the relationship between Anammox reactor performance and microbial community succession.

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Identification and quantification of anammox bacteria in eight nitrogen removal reactors

Cited by 169 publications

References 37 publications

Performance and microbial community of simultaneous anammox and denitrification (SAD) process in a sequencing batch reactor

Performance and microbial community of simultaneous anammox and denitrification (SAD) process in a sequencing batch reactor

Nitrogen removal through different pathways in an aged refuse bioreactor treating mature landfill leachate

Characterization of a Microbial Community in an Anammox Process Using Stored Anammox Sludge

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