Identification and quantification of 13 components in Angelica sinensis (Danggui) by gas chromatography–mass spectrometry coupled with pressurized liquid extraction

Lao, Sin-Cheng; Li, Shaoping; Kan, Koichi; Li, Peng; Wan, Jian‐Bo; Wang, Ying; Dong, Tina Tingxia; Tsim, Karl Wah Keung

doi:10.1016/j.aca.2004.09.050

Cited by 111 publications

(71 citation statements)

References 9 publications

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“…Therefore, the demand for Angelica sinensis is enormous [Sarker and Nahar, 2004]. In order to control the quality of Angelica sinensis and protect the safety of the consumers, we conducted a study to compare quantitatively several chemical components of different types of Angelica sinensis [Lao et al, 2004]. Identification of bioactive components in Angelica sinensis is very important not only for the quality control of crude botanical drugs but also for elucidating the therapeutic principle.…”

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confidence: 99%

The angiogenic effects of Angelica sinensis extract on HUVEC in vitro and zebrafish in vivo

Lam

Lin

Lao

et al. 2007

J of Cellular Biochemistry

109

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Angiogenesis plays an important role in a wide range of physiological processes such as wound healing and fetal development. Many diseases are associated with imbalances in regulation of angiogenesis, in which it is either excessive or there is insufficient blood vessel formation. Angelica sinensis (AS), commonly used in the prescriptions of Chinese medicine, is a potential candidate for curing such diseases. However, biological effects of AS on angiogenesis and underlying mechanisms are yet to be fully elucidated. This investigation describes the angiogenic effects of AS extract on human endothelial cells (HUVEC) in vitro and zebrafish in vivo. The extract was demonstrated, by XTT assay and microscopic cell counting, to stimulate the proliferation of HUVEC; in addition, flow cytometry analysis indicated that the extract increased the percentage of HUVEC in the S phase. The wound healing migration assay illustrated that a dramatic increase in migration could be measured in AS extract-treated HUVEC. Meanwhile, the number of invaded cells and the mean tube length were significantly increased in AS extract treatment groups. The extract was also demonstrated to promote changes in subintestinal vessels (SIVs) in zebrafish, one feature of angiogenesis. In addition, AS extract was found by real-time PCR to enhance vascular endothelial growth factor (VEGF) mRNA expression. In a bead-based immunoassay, higher levels of p38 and JNK 1/2 expression were also observed in effusions compared with control cells. All results suggest that Angelica sinensis extract can promote angiogenesis, and that the angiogenic effects involve p38 and JNK 1/2 phosphorylation.

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confidence: 99%

The angiogenic effects of Angelica sinensis extract on HUVEC in vitro and zebrafish in vivo

Lam

Lin

Lao

et al. 2007

J of Cellular Biochemistry

109

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“…23 Extracts of the root of AS have been reported to have beneficial effects on human vascular endothelium affected by oxidized low density lipoprotein. 10 The active ingredients of AS root, determined by gas chromatography-mass spectroscopy, are thought to be ferulic acid, ligustilide and other phthalides such as butylidenephthalide, but others may be involved in AS activity.…”

Section: Discussionmentioning

confidence: 99%

“…The levels of these constituents are known to be variable, depending on the region and climate where the plants were grown. 23 Nonetheless AS root, known best as Danggui, is in great demand throughout the world.…”

Section: Discussionmentioning

confidence: 99%

Angelica sinensis has inherent endothelial cell toxicity at high concentrations but can also protect the vascular endothelium from oxidative stress-induced injury at moderate concentrations

et al. 2011

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Hypoxia and oxidative stress are important factors in the pathogenesis of many acute forms of injury, especially acute kidney injury. Apoptosis is a key mode of endothelial cell death from oxidative stress. Minimising the detrimental effects of oxidative stress is necessary to reduce injury, and new treatment strategies are constantly being sought. The aim of this study was to investigate the ability of an aqueous/methanol extract of Angelica sinensis (AS) root (Chinese names Danggui, Dong quai, Donggui) to protect endothelium from hypoxia and oxidative stress. This was compared in specialised kidney endothelium (renal medullary vascular endothelial cells; RMVEC) versus central endothelium (aortic endothelial cells; AEC). Toxicity of various strengths of AS was first tested in RMVEC and AEC using % apoptosis and mitosis as outcomes. Morphological and molecular characteristics of apoptosis and mitosis, and the effect of AS on heme-oxygenase-1, a marker of cellular response to oxidative stress, were also investigated. The results showed that, at concentrations of 2500µg/mL or greater, AS significantly increased apoptosis in RMVEC and AEC (P<0.05), however concentrations of 2000 µg/mL or less were non-toxic and also nonmitogenic. Endothelial cells were then treated with hydrogen peroxide (0.8 mM for RMVEC; 0.6 mM for AEC) for oxidative stress, with and without 2000 µg/mL AS. AS significantly inhibited oxidant-induced apoptosis (P<0.05) but had little effect on mitosis. AS also increased heme-oxygenase-1, but only in AEC. AS extracts may have some inherent toxicity at high concentrations, but with careful analysis of non-toxic levels, both renal and central endothelium benefited from AS against oxidative stress-induced apoptosis, without inducing excessive mitosis, and AS may find application in some oxidant-induced disease.

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“…They have smooth muscle relaxant activity , vasorelaxing activity (Chan et al 2006), analgesic, neuroprotective (Peng et al 2007; Kuang et al 2006), antisclerotic, antihypertensive (Lu et al 2006), serotonergic (Deng et al 2006a), GABAergic (Deng et al 2006b), antiproliferative (Liang and He 2006), antihyperglycemic (Brindis et al 2011), sedative and spasmolytic (León et al 2011). These compounds have been found in some genera of the Apiaceae including Angelica (Lao et al 2004), Anethum (Fischer and Gijbels 1987), Apium (MacLeod et al 1988), Bifora (Gijbels et al 1985), Capnophyllum (Gijbels et al 1984), Cnidium (Gijbels et al 1981), Conioselinum (Gijbels et al 1979), Levisticum (Santos et al 2005), Ligusticum (Delgado et al 1988), Lomatium (Bairamian et al 2004), Opopanax (Gijbels et al 1983), Petroselinum (Gijbels et al 1985), Peucedanum (Gijbels et al 1984) and Meum (Palá-Paúl et al 2004).…”

Section: Introductionmentioning

confidence: 99%

In vitro morphogenetic responses and comparative analysis of phthalides in the highly valued medicinal plant Ligusticum porteri Coulter & Rose

et al. 2012

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The morphogenetic response of Ligusticum porteri, a medicinal and ceremonial plant, was investigated as part of the conservation strategy of this wild species and was compared to that of a cultivated species, Petroselinum crispum. Seeds were germinated in half strength Murashige and Skoog medium. Plantlets were excised into root, cotyledon, petiole, stem and leaf explants and cultured in an induction medium supplemented with the range of 0-18.09 lM 2,4-dichlorophenoxyacetic acid (2,4-D) or 0-21.48 lM a-naphthaleneacetic acid in combination with 0-13.31 lM 6-benzylaminopurine. Calli derived from leaf, seeds, petiole, stem and roots, mature aerial parts and roots extracts of L. porteri and P. crispum were analyzed by thin layer chromatography and gas chromatography coupled to mass spectrometry. 3-Butylidenephthalide (6.3%) was identified along with other 23 compounds from mature aerial parts extract of L. porteri and also in its roots (20.8%). 3-nButylphthalide (0.7%) and 3,6,7,-trimethoxy-isobenzofuran-13(H)-one (4.9%) were identified from the roots of P. crispum.3-Butylidenephthalide was identified from two petiole (0.9%; 0.26%) and one stem (0.8%) callus extracts of L. porteri. This is the first report on phthalides production from in vitro cultures of L. porteri. The results indicated that in vitro cultures of this plant possess the biosynthetic machinery for the biosynthesis of these highly valuable compounds.

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Identification and quantification of 13 components in Angelica sinensis (Danggui) by gas chromatography–mass spectrometry coupled with pressurized liquid extraction

Cited by 111 publications

References 9 publications

The angiogenic effects of Angelica sinensis extract on HUVEC in vitro and zebrafish in vivo

The angiogenic effects of Angelica sinensis extract on HUVEC in vitro and zebrafish in vivo

Angelica sinensis has inherent endothelial cell toxicity at high concentrations but can also protect the vascular endothelium from oxidative stress-induced injury at moderate concentrations

In vitro morphogenetic responses and comparative analysis of phthalides in the highly valued medicinal plant Ligusticum porteri Coulter & Rose

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