Identification and purification of the specific antigen of<i>Paracoccidioides brasiliensis</i>responsible for immunoelectrophoretic band E

Yarzábal, L. A.; Bout, Daniel; Naquira, F.; Fruit, J.; Andrieu, S

doi:10.1080/00362177785190131

Cited by 46 publications

(18 citation statements)

References 6 publications

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“…It is possible for different antigenic preparations to vary considerably in activity and quality as a result of lack of proper lotto-lot production standards even within the same laboratory. With this variety of problems in producing a proper P. brasiliensis antigen, it is not surprising that there is considerable disagreement regarding the sensitivity of the ID test for the serodiagnosis of PCM [5][6][7][8][9][10][11].…”

Section: Antigen Preparationmentioning

confidence: 99%

Serology of paracoccidioidomycosis

Camargo

2008

Mycopathologia

113

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This review provides the background for understanding the role of a battery of diagnostic methods in paracoccidioidomycosis (PCM). This systemic mycosis is a disease endemic in many regions of Latin America, with sporadic cases also occurring throughout the world (mycosis of importation). Although excellent laboratory methods for diagnosis are available, there are deficiencies that must be met by continued research. Understanding the uses and limitations of a battery of laboratory methods is essential to diagnose PCM. Clinicians and laboratory directors must be familiar with the uses and limitations of a battery of serologic and mycological tests to accurately diagnose of PCM. Antibody and antigen detections are valuable adjuncts to histopathology and culture. More recently, the gp43 and gp70 antigen detection assay have improved the methodology of diagnosis of this mycosis, which improves reproducibility and facilitates monitoring antigen clearance during antifungal treatment. Furthermore, detection of antigen in cerebrospinal fluid and in bronchoalveolar lavage fluid increases the sensitivity for diagnosis of PCM in central nervous system and in pulmonary infections, respectively.

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Section: Antigen Preparationmentioning

confidence: 99%

Serology of paracoccidioidomycosis

Camargo

2008

Mycopathologia

113

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“…This glycoprotein is the major antigenic component of P. brasiliensis, since 100% of PCM patient sera display antibodies against it. gp43 is a high-mannose concanavalin A-binding glycoprotein (30,32,33,39,43). Several biological functions have been proposed for this molecule.…”

mentioning

confidence: 99%

Anti-Idiotypic Antibodies in Patients with Different Clinical Forms of Paracoccidioidomycosis

Souza

Gesztesi

Negro

et al. 2000

Clin Diagn Lab Immunol

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“…Antisera were prepared against the C 2 antigen of A.fumigatus (15), and the E2 of P. brasiliensis (20), using the procedure described by Goudie, Horne & Wilkinson, (5) as modified by Bout et al (3). Briefly, the precipitation arcs corresponding to the chosen antigens were identified and excised from immunoelectrophoresis slides which have been prepared with the crude antigen and the corresponding homologous antisera.…”

Section: Exp3rimenxai Monospecific Antiseramentioning

confidence: 99%