Identification and Purification of Potential Bioactive Peptide of Moringa oleifera Seed Extracts

Abstract

The aim of the study was to investigate the antibacterial and anticoagulant activity of Moringa (Moringa oleifera) seed extracts and coagulant protein for their potential application in water treatment. Pathogenic microorganisms were obtained from Ramachandra Hospital, Chennai, India. Bacterial cell aggregation and growth kinetics studies were employed for six bacterial strains with different concentrations of seed extracts and coagulant protein. Moringa seed extract and coagulant protein showed cell aggregation against six bacterial strains, whereas seed extract alone showed growth inhibition of all six bacterial strains for up to 6 h, compared to that of control. Escherichia coli and Salmonella para typhi B did not develop resistance against coagulant protein. The results imply that Moringa oleifera is likely an efficient low-molecular bioactive peptide (with <7.5 kDa plant-based coagulant and antimicrobial peptides, confirmed by applying amino acid sequences), using liquid chromatography–mass spectrometry and HPLC, with the corresponding sequences from Napin-1A peptide posing different degrees of antibacterial activity against different pathogenic organisms.

Characterization of a Novel Antimicrobial Peptide Isolated from Moringa oleifera Seed Protein Hydrolysates and Its Membrane Damaging Effects on Staphylococcus aureus

“…Similarly, Chandrashekar et al isolated Napin-1A peptide from M. oleifera seeds with antibacterial and anticoagulant activities . Shebek et al reported that a cationic protein (MOCP) isolated from M.…”

“…14 Similarly, Chandrashekar et al isolated Napin-1A peptide from M. oleifera seeds with antibacterial and anticoagulant activities. 15 Shebek et al reported that a cationic protein (MOCP) isolated from M. oleifera seeds could inhibit the growth of Escherichia coli(E. coli) by membrane fusion. 16 Dasgupta et al (b) proceeded with the incorporation of the antimicrobial MOCP into the blotting paper to "kill" the "trapped" E. coli through prolonged contact, which effectively enhanced the role of MOCP in water purification.…”

Moringa oleifera leaf as a natural water purifier and causes decontamination of fecal-coliform bacteria

“…In addition Xiong et al, (2018) investigated the use of MOL as beds to disinfect the raw water from E. coli, which exhibited a high removal efficiency of 99.0 %, with a total coliform count of 3-4 cfu\ 100 ml. In the same line, Chandrashekar et al, (2020); Begum et al, (2021) indicated that a crude extract from Moringa contained a coagulant protein that exhibited cell aggregation and growth inhibition of E. coli and S. paratyphi B coliform bacteria, which was attributed to the presence of peptides with antibacterial properties in the Moringa extract. In a recent study conducted by Semanka et al, (2022), it was observed that reduction of coliform count recorded more than 65 % of the total coliform.…”

Moringa oleifera: a valuable insight into recent advances in medicinal uses and pharmacological activities

“…The ethanolic extracts of the leaves also show antibiotic activity against a variety of microorganisms, like Pseudomonas aeruginosa , Salmonella typhi , Vibrio cholera , and Escherichia coli 62 . Numerous studies propose that the seed extracts of M. oleifera have a likelihood of being used in water purification because it inhibited the growth of bacteria in both agar and nutrient media 63 . Methanol extracts of the leaves repressed pathogens of the urinary tract, such as of Staphylococcus aureus , Klebsiella pneumonia .…”

“…62 Numerous studies propose that the seed extracts of M. oleifera have a likelihood of being used in water purification because it inhibited the growth of bacteria in both agar and nutrient media. 63 Methanol extracts of the leaves repressed pathogens of the urinary tract, such as of Staphylococcus aureus, Klebsiella pneumonia. One article evaluated the antimicrobial property of M. oleifera leaf ethanolic extract against Enterobacter spp.…”