Identification and preparation of selenium‐containing peptides from selenium‐enriched Pleurotus eryngii and their protective effect on lead‐induced oxidative damage in NCTC1469 hepatocytes

Abstract

BACKGROUND Lead (Pb) is a highly toxic and persistent substance that easily accumulates in living organisms, eliciting cellular toxicity and oxidative stress. Some selenium‐containing proteins and peptides prepared from plant extracts are beneficial for protecting the body's health and resisting external disturbances. In the present study, selenium‐containing peptide species were prepared from selenium‐enriched Pleurotus eryngii protein hydrolysates and to evaluate the benefits of selenium‐containing peptides on Pb‐induced oxidative stress in NCTC1469 hepatocytes. RESULTS Trypsin was selected as primary enzyme to hydrolyze the selenium‐enriched protein (SPH). The optimal hydrolysis conditions were: hydrolysis time, 1.5 h; initial pH 8.0. The SPH was digested by trypsin and then purified by ultrafiltration, gel filtration chromatography and reversed‐phase HPLC to obtain the selenium‐containing peptides SPH‐I‐2. Furthermore, SPH‐I‐2 was analyzed and a number of total 12 selenium‐containing peptides were identified by liquid chromatography‐tandem mass spectroscopy. The NCTC1469 cell culture study showed that selenium‐containing peptides were capable of reducing reactive oxygen species levels and regulating the Keap1/Nrf2 pathway by upregulating Nrf2, HO‐1, GCLC, GCLM and NQO1 genes and downregulating Keap1 genes. Moreover, selenium‐containing peptides were also able to suppress Pb‐induced elevated levels of nitric oxide (NO), lactate dehydrogenase (LDH), malondialdehyde (MDA), increase antioxidant enzyme activity and alleviate cell apoptosis. CONCLUSION The present study indicated that the selenium‐containing peptides could protect cells from Pb2+‐induced oxidative stress. © 2023 Society of Chemical Industry.

Antimicrobial Activity of Grapefruit Seed Extract on Edible Mushrooms Contaminations: Efficacy in Preventing Pseudomonas spp. in Pleurotus eryngii

“…These characteristics have aroused particular interest because of the anti-cancer, hepatoprotective, anti-lipidemia, immune system strengthening, and other a good concentration of major nutrients, such as protein, peptides, minerals, terpenoids, traces of various elements, fiber, and polysaccharides (P. eryngii polysaccharides-PEPs). These characteristics have aroused particular interest because of the anti-cancer, hepatoprotective, anti-lipidemia, immune system strengthening, and other activities shown in vitro and in animal models [6][7][8][9][10]. For this reason, P. eryngii is classified among functional foods [11][12][13] and used to create "healthy snacks" [14], namely, foods with high nutritional and biological value (rich in fiber and protein, low in salt, sugar, fat, and calories) [11].…”

Identification of novel antioxidant collagen peptides for preventing and treating H2O2‐induced oxidative stress in HepG2 cells through in vitro and in silico approaches

“…In recent years, food‐derived peptides have attracted immense attention due to their extensive sources and desirable multiple bioactivities 7,8 . Scientific evidence has suggested that food‐derived peptides are safer than synthetic drugs, and it is highly desirable to employ food‐derived peptides with antioxidant activity to attenuate the risk of occurrence and aggravation of chronic diseases 9,10 …”

“…7,8 Scientific evidence has suggested that foodderived peptides are safer than synthetic drugs, and it is highly desirable to employ food-derived peptides with antioxidant activity to attenuate the risk of occurrence and aggravation of chronic diseases. 9,10 Collagen is a favorable source of bioactive substances, which can release peptides with higher antioxidant activity after enzymatic hydrolysis. 11 Up to now, a series of antioxidant collagen peptides have been isolated and identified, which can exhibit different degrees of free radical scavenging activity.…”

Effect of Lead Toxicity on Japanese Quail and its Alleviation with Dietary Selenium

“…Selenium is found in a variety of selenoproteins, including glutathione peroxidases (GPx), which are antioxidant enzymes found in both animals and humans (Savitha et al, 2014). Protective effects of selenium against acute and chronic toxicity has been well established in the literature (Qiao et al, 2022;Yu et al, 2023). It has been reported that like other poultry species, quails are extremely vulnerable to lead poisoning, altering the weight of the body and its organs as well as the blood, serum biochemistry and immunity (Wang et al, 2022).…”