Identification and preliminary characterization of non-polyene antibiotics secreted by new strain of actinomycete isolated from sebkha of Kenadsa, Algeria

Abstract

A B S T R A C TObjective: To study the antimicrobial activity of actinomycetes isolated from sabkha of Kenadsa and identification of the isolate interesting. Methods: Eighteen strains were isolated, using four culture media from sebkha of Kenadsa (Bechar, Southwestern Algeria). Screening of antimicrobial activity consisted of two steps: in primary screening, antibacterial activity was determined by using the agar plug method against test strains; in secondary screening, better isolate which showed a good activity in the first screening was selected to extract antimicrobial substances. The antimicrobial activities of extracts were evaluated by using Kirby-Bauer disc diffusion method. Partial characterization of antimicrobial products was performed on the basis of chemical revelations, UV-vis spectrometry and infrared spectroscopy. The identification of isolate interesting was performed through morphological, chemical, biochemical and physiological characteristics. Results: All isolates showed antimicrobial activity against at least one microorganism test. One isolate, LAM143cG3, was selected for its broad spectrum and high antimicrobial activity. The isolate LAM143cG3 was identified as Spirillospora sp. The comparison between the species of this genus (Spirillospora rubra and Spirillospora albida) and our isolate indicated the existence of several physiological and biochemical differences which led us to suppose that this was a new member of this genus. Primary characterization of antimicrobial substances produced by the isolate LAM143cG3 indicated the presence of amines and phenols. The UV-vis spectrum suggested a nonpolyenic nature of substances secreted by our isolate, while infrared confirmed the presence of amine groups. Conclusions: The result of the present study revealed that sebkha of Kenadsa was rich in rare actinomycetes, that secreted interesting antimicrobial substance.

Antimicrobial Properties of Actinomycetes Isolated from Menengai Crater in Kenya

“…Actinomycetes are said to be morphologically, physiologically and ecologically diverse organisms [7]. They are of great significance to the pharmaceutical world due to their ability to produce secondary metabolites of medical importance [8].…”

Microbiological and molecular insights on rare Actinobacteria harboring bioactive prospective

“…Originally, classical approaches for the identification of Actinobacteria were based on morphological observations, chemotaxonomy, and physiological criteria. Morphological observations were performed to identify an unknown strain to the genus level including identification of aerial mycelium presence, color of substrate mycelium, color of aerial mycelium, ornamentation of spores, and production of soluble pigments (Barka et al, 2016;Shirling & Gottlieb, 1966;Messaoudi et al, 2015;Amin et al, 2017a). Chemotaxonomic criteria such as the detection of diaminopimelic acid (DAP) isomers are one of the most important cell wall properties of Actinobacteria.…”

Antibacterial metabolite prospecting from Actinomycetes isolated from waste damped soils from Thika, central part of Kenya

“…The composite soil samples were air dried in this lab at room temperature according to Azira et al [5] and Messaoudi et al [16]. The soil samples were transported to Kenya Industrial research Development and Innovation (KIRDI).…”

“…Soil samples from each plot were collected aseptically from 0-20 cm depth [14] at depth of 0-5 cm, 5-10 cm, 10-15 cm [15]and 15-20 cm [16]. Four composite soil samples were prepared based on depth per site and the samples were packed using sterile polyethylene bag [5,15,16] to minimize soil moisture lose. From 8 sites 32 composite soil samples were prepared, packed and transported to Jomo Kenyata University of Agriculture and Technology (JKUAT), Zoology laboratory.…”

“…The soil samples were transported to Kenya Industrial research Development and Innovation (KIRDI). Dry heat pretreatment of composite soil sample was done according to Azira et al [5] and chemical treatment using calcium carbonate or CaCO 3 (1%) was done according to Messaoudi et al [16].…”