IDENTIFICATION AND PATHOGENICITY TEST OF SOME BACTERIA ISOLATED FROM WILD AND FARMED SPINY LOBSTER Panulirus homarus

Abstract

The bacterial populations in the farming of spiny lobster could have either beneficial or harmful effects depending on the prevailing conditions. We designed this study to identify and to perform a pathogenicity test of some bacteria isolated from wild and farmed spiny lobsters Panulirus homarus. The adult farmed lobsters were obtained from Pangandaran and Lombok coastal areas, while the wild lobsters were collected from Lombok, with five lobsters for each location. The bacteria were isolated from the midgut, gill, hepatopancreas, and muscle tissues of the lobsters. The identification of the bacteria was carried out by molecular methods. Pathogenicity test was performed by intramuscular injection of 0.1 ml bacterial suspensions at the density of 7×106cfu/ml into each three adult apparently healthy lobsters for every eight bacterial isolates. Our study identified six bacterial isolates that exhibited high homology of a nucleotide sequence with Shewanella algae, Bacillus firmus, Vibrio alginolyticus, Tenacibaculum lutimaris, Pseudomonas sp. and Vibrio sp., while two isolates were reminded unidentified due to low nucleotide similarities (< 97%). The pathogenicity test showed that there was no mortality of lobsters injected with those bacterial isolates. This may because the dose of injection was too low to induce bacterial infection particularly for Vibrio, or the bacteria were not pathogenic for lobster or even have the potency as probiotic bacteria.

Identification of Vibrio spp. in Spiny Lobster (Panulirus homarus) from Natural Catch and Culture In Batu Bangka Village, Sumbawa

“…In some cases, it also shows the color of the hepatopancreas greatly affects health status. Based on findings on several lobsters that identified symptoms of milky disease had the appearance of white hepatopancreas (Sudewi et al, 2019). Nur and Yusnaini (2018) hepatopancreas is an organ sensitive to changes in the environment, food, or health status.…”

“…Cultivation activities have so far been carried out on several fishery commodities, while the conversion of mangrove land and community settlements are several factors that influence the success of cultivation on Bungin Island. Sudewi et al (2019) apart from the injection method, exposure to bacteria can also be through maintenance media, although the potential infection rate is low. Meanwhile, direct contact through cannibalism can increase the risk of infection.…”

“…the form of pictures and tables. Data collection was carried out to determine the types of pathogenic bacteria that can attack the spiny lobster (P. homarus) (Sudewi et al, 2019).…”

“…Meanwhile, the results of the identification of pathogenic bacteria caught in nature include Shewanella, Bacillus firmus, Vibrio alginolyticus, Tenacibaculum lutimaris, Lysinibacillus fusiformis, and Pseudomonas sp. (Sudewi et al, 2019).…”