Identification and multi-environment validation of resistance to Botrytis fabae in Vicia faba

Villegas-Fernández, Ángel M.; Sillero, Josefina C.; Emeran, Amero A.; Winkler, Johanna; Raffiot, Blandine; Tay, J.; Flores, Fernando; Rubiales, Diego

doi:10.1016/j.fcr.2009.07.005

Cited by 45 publications

(34 citation statements)

References 25 publications

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“…Incomplete resistance to Botrytis fabae has been reported in faba bean germplasm (Bond et al, 1994;Hanounik and Robertson, 1988;Villegas et al, 2009;2012). No QTLs or associated molecular markers have been reported so far, although molecular characterization of the resistance is in progress (Villegas et al, 2014).…”

Section: Downloaded By [West Virginia University] At 06:40 20 Novembementioning

confidence: 96%

Achievements and Challenges in Legume Breeding for Pest and Disease Resistance

Rubiales

Fondevilla

Cao

et al. 2014

Critical Reviews in Plant Sciences

176

132

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Section: Downloaded By [West Virginia University] At 06:40 20 Novembementioning

confidence: 96%

Achievements and Challenges in Legume Breeding for Pest and Disease Resistance

Rubiales

Fondevilla

Cao

et al. 2014

Critical Reviews in Plant Sciences

176

132

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“…They may be proposed as promising sources of resistance to chocolate spot in this area of Spain. Only four of them (132-1, 135-1, BPL 710, ILB 4726) had been included among the group of ten accessions identified as interesting resistant sources in a previous multi-environment test (Villegas-Fernández et al 2009). Thus, the whole plant test in this work has allowed us to fine-tune that previous selection process and choose those accessions that are best suited for the particular environment at Cordoba and, additionally, to identify two new sources of resistance that would have been otherwise overlooked.…”

Section: Discussionmentioning

confidence: 99%

“…For this purpose, a germplasm collection of 42 accessions of Vicia faba that had shown responses of interest to Botrytis fabae in previous studies (Villegas-Fernández et al 2009) was made use of. The accessions were evaluated in the adult plant stage in the field and in the seedling stage under controlled conditions using detached leaflet and whole plant tests.…”

Section: Introductionmentioning

confidence: 99%

Screening faba bean for chocolate spot resistance: evaluation methods and effects of age of host tissue and temperature

2011

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“…The timely application of fungicides with demonstrated efficacy against chocolate spot is also an important disease control practice (Sahile et al, 2008b;Stoddard et al, 2010). Only a few sources of genetic resistance to chocolate spot have been identified in faba bean germplasm (Hanounik and Robertson, 1988;Bouhassan et al, 2004;Villegas-Fernández et al, 2009;Maalouf et al, 2016). However, resistant varieties that are adapted to a range of growing regions have not yet been made widely available to farmers (Sillero et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Characterization of Growth Morphology and Pathology, and Draft Genome Sequencing of Botrytis fabae, the Causal Organism of Chocolate Spot of Faba Bean (Vicia faba L.)

et al. 2020

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Chocolate spot is a major fungal disease of faba bean caused by the ascomycete fungus, Botrytis fabae. B. fabae is also implicated in botrytis gray mold disease in lentils, along with B. cinerea. Here we have isolated and characterized two B. fabae isolates from chocolate spot lesions on faba bean leaves. In plant disease assays on faba bean and lentil, B. fabae was more aggressive than B. cinerea and we observed variation in susceptibility among a small set of cultivars for both plant hosts. Using light microscopy, we observed a spreading, generalized necrosis response in faba bean toward B. fabae. In contrast, the plant response to B. cinerea was localized to epidermal cells underlying germinated spores and appressoria. In addition to the species characterization of B. fabae, we produced genome assemblies for both B. fabae isolates using Illumina sequencing. Genome sequencing coverage and assembly size for B. fabae isolates, were 27x and 45x, and 43.2 and 44.5 Mb, respectively. Following genome assembly and annotation, carbohydrate-active enzyme (CAZymes) and effector genes were predicted. There were no major differences in the numbers of each of the major classes of CAZymes. We predicted 29 effector genes for B. fabae, and using the same selection criteria for B. cinerea, we predicted 34 putative effector genes. For five of the predicted effector genes, the pairwise dN/dS ratio between orthologs from B. fabae and B. cinerea was greater than 1.0, suggesting positive selection and the potential evolution of molecular mechanisms for host specificity in B. fabae. Furthermore, a homology search of secondary metabolite clusters revealed the absence of the B. cinerea phytotoxin botrydial and several other uncharacterized secondary metabolite biosynthesis genes from B. fabae. Although there were no obvious differences in the number or proportional representation of different transposable element classes, the overall proportion of AT-rich DNA sequence in B. fabae was double that of B. cinerea.

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Identification and multi-environment validation of resistance to Botrytis fabae in Vicia faba

Cited by 45 publications

References 25 publications

Achievements and Challenges in Legume Breeding for Pest and Disease Resistance

Achievements and Challenges in Legume Breeding for Pest and Disease Resistance

Screening faba bean for chocolate spot resistance: evaluation methods and effects of age of host tissue and temperature

Characterization of Growth Morphology and Pathology, and Draft Genome Sequencing of Botrytis fabae, the Causal Organism of Chocolate Spot of Faba Bean (Vicia faba L.)

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