Identification and molecular mapping of Rps11, a novel gene conferring resistance to Phytophthora sojae in soybean

Abstract

Phytophthora root and stem rot (PRSR), caused by the soil-borne pathogen Phytophthora sojae, is a devastating disease of soybean [Glycine max (L.) Merr.] throughout the world. Deploying resistant soybean cultivars is the most effective and environmentally friendly approach to managing this disease. The soybean landrace PI 594527 was found to carry excellent resistance to all P. sojae isolates examined, some of which were capable of overcoming the major Rps genesp, such as Rps1-k, Rps1-c, and Rps3-a, predominantly used for soybean protection in the past decades. A mapping population consisting of 58 F2 individuals and 209 F2:3 families derived from a cross between PI 594527 and the susceptible cultivar 'Williams' was used to characterize the inheritance pattern of the resistance to P. soja (Rps) in PI 594527. It was found that the resistance was conferred by a single Rps gene, designated Rps11, which was initially defined as an ~5 Mb genomic region at the beginning of chromosome 7 by bulked segregant analysis (BSA) with a nucleotide polymorphism (SNP) chip comprising 7039 SNP markers. Subsequently, simple sequence repeat (SSR) markers in the defined region were used to genotype the F2:3 mapping population to map Rps11 to a 225.3 kb genomic region flanked by SSR markers BARCSOYSSR_07_0286 and BARCSOYSSR_07_0300, according to the soybean reference genome sequence. Particularly, an SSR marker (i.e., BARCSOYSSR_07_0295) was found to tightly co-segregate with Rps11 in the mapping population and can be effectively used for marker-assisted selection of this gene for development of resistant soybean cultivars.

Fine Mapping of a Resistance Gene RpsHN that Controls Phytophthora sojae Using Recombinant Inbred Lines and Secondary Populations

“…Each family had 30 plants scored. The standard criterion of each family is as follows: if the percentage of dead seedlings >80%, then this family was recorded as homozygous susceptible (S); if the percentage of dead seedlings <20%, then this family was recorded as homozygous resistant (R); and if the percentage of dead seedlings is between 21 and 79%, then this family was recorded as heterozygous resistant (Rs) (Gordon et al, 2006; Ping et al, 2015). …”

“…To our knowledge, 22 race-specific resistance soybean cultivars containing single genes have been identified and reported: L88-8470 ( Rps1a ), L77-1863 ( Rps1b ), L75-3735 ( Rps1c ), L93-3312 ( Rps1d ), L77-1794 ( Rps1k ), L76-1988 ( Rps2 ), L83-570 ( Rps3a ), L91-8347 ( Rps3b ), L92-7857 ( Rps3c ), L85-2352 ( Rps4 ), L85-3059 ( Rps5 ), L89-1581 ( Rps6 ), L93-3258 ( Rps7 ), PI 399073 ( Rps8 ), Ludou4 ( Rps9 ), Wandou15 ( Rps10 ), PI 594527 ( Rps11 ), PI 567139B ( RpsUN1 and RpsUN2 ), Yudou25 ( RpsYu25 ), Yudou29 ( RpsYD29 ), Waseshiroge (unnamed Rps gene), and Nannong 10-1 ( RpsJS ) (Sugimoto et al, 2011, 2012; Sun et al, 2011, 2014; Wu et al, 2011; Zhang et al, 2013a,b; Ping et al, 2015; Li et al, 2016). Twenty-three genes/alleles identified in the soybean cultivars listed above were located on six chromosomes.…”

“…RpsJS , a fine mapping gene located in a 138.9-kb region with 14 candidate genes on chromosome 18, and three genes Glyma18g51930, Glyma18g51950 and Glyma18g51960 were characterized as NBS-LRR type genes (Sun et al, 2014). Rps11 mapped to a 225.3-kb region on chromosome 7, and Rps10 mapped to a 311-kb region on chromosome 17 (Zhang et al, 2013a; Ping et al, 2015). Furthermore, the Rps10 mapping region contained two candidate genes, Glyma17g28950.1 and Glyma17g28970.1 , annotated as serine/threonine (Ser/Thr) protein kinases.…”

Fine mapping of a Phytophthora-resistance gene RpsWY in soybean (Glycine max L.) by high-throughput genome-wide sequencing

“…Rps3 (three alleles Rps3a, Rps3b and Rps3c ) and RpsSN10 which was linked with Rps8 were on chromosome 13 (Demirbas et al 2001; Sandhu et al 2005; Gordon et al 2006; Yu et al 2010). In addition, Rps4, Rps5, Rps6 , and RpsJS were located on chromosome 18 (Demirbas et al 2001; Sandhu et al 2004; Sun et al 2014a, b), and Rps8, RpsYB30, RpsZS18, RpsSu, Rps10 , and Rps11 were located on chromosome 8, 19, 2, 10, 17 and 7, respectively (Burnham et al 2003a; Zhu et al 2007; Yao et al 2010; Wu et al 2011b; Zhang et al 2013c; Ping et al 2016). …”

A Novel Phytophthora sojae Resistance Rps12 Gene Mapped to a Genomic Region That Contains Several Rps Genes

“…In fact, Rps4 and Rps6 could be allelic [50]. Rps10 has been mapped to Chromosome 17 [55], RpsYB30 and RpsZS18 [56–57] to Chromosome 19 and Chromosome 2, respectively, and Rps11 to Chromosome 7 [58]. …”