Identification and Molecular Mapping of a Gummy Stem Blight Resistance Gene in Wild Watermelon (<i>Citrullus amarus</i>) Germplasm PI 189225

Ren, Runsheng; Xu, Jianfei; Zhang, Man; Guang, Liu; Yao, Xiefeng; Zhu, Lin; Hou, Qi

doi:10.1094/pdis-04-19-0753-re

Cited by 29 publications

(49 citation statements)

References 56 publications

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“…Gusmini et al (2005) reported a low correlation (r = 0.10 -0.36) in the evaluation for GSB resistance in watermelon. More recently, Ren et al (2019) reported a significantly high correlation (r = 0.92) for GSB disease incidence in watermelon seedlings between two greenhouse experiments. We calculated a relatively high (72.6%) broad sense heritability, with no significant interaction observed between the genotype × experiment.…”

Section: Discussionmentioning

confidence: 95%

“…The total number of genes in the 2-LOD confidence interval for each QTL were: ClGSB3.1: 65; ClGSB5.1: 712; ClGSB7.1: 574 (Electronic Supplementary Material 4). The GSB resistance loci and candidate genes identified in the present study were compared with those identified previously in cucurbit species (Lou et al 2013;Liu et al 2017;Zhang et al 2017;Hassan et al 2018;Hu et al 2018;Ren et al 2019).…”

Section: Candidate Gene Identificationmentioning

confidence: 99%

“…Initial studies of GSB resistance in PI 189225 reported that resistance was mediated by a single gene, db (Norton 1979). However, later studies on PI 189225, PI 482283, and PI 526233 found that many genes with minor effects are most likely responsible for this trait (Gusmini et al 2017;Hassan et al 2019;Ren et al 2019). Recently, a quantitative trait locus (QTL) underlying GSB resistance in PI 189225 was described on chromosome 8 of watermelon (Ren et al 2019).…”

Section: Electronic Supplementary Materialsmentioning

confidence: 99%

“…However, later studies on PI 189225, PI 482283, and PI 526233 found that many genes with minor effects are most likely responsible for this trait (Gusmini et al 2017;Hassan et al 2019;Ren et al 2019). Recently, a quantitative trait locus (QTL) underlying GSB resistance in PI 189225 was described on chromosome 8 of watermelon (Ren et al 2019). This QTL explains ~ 32% of the phenotypic variance in the population.…”

Section: Electronic Supplementary Materialsmentioning

confidence: 99%

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QTL associated with gummy stem blight resistance in watermelon

et al. 2020

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Key message We identified QTLs associated with gummy stem blight resistance in an interspecific F2:3Citrullus population and developed marker assays for selection of the loci in watermelon. Abstract Gummy stem blight (GSB), caused by three Stagonosporopsis spp., is a devastating fungal disease of watermelon (Citrullus lanatus) and other cucurbits that can lead to severe yield losses. Currently, no commercial cultivars with genetic resistance to GSB in the field have been reported. Utilizing GSB-resistant cultivars would reduce yield losses, decrease the high cost of disease control, and diminish hazards resulting from frequent fungicide application. The objective of this study was to identify quantitative trait loci (QTLs) associated with GSB resistance in an F2:3 interspecific Citrullus mapping population (N = 178), derived from a cross between Crimson Sweet (C. lanatus) and GSB-resistant PI 482276 (C. amarus). The population was phenotyped by inoculating seedlings with Stagonosporopsis citrulli 12178A in the greenhouse in two separate experiments, each with three replications. We identified three QTLs (ClGSB3.1, ClGSB5.1 and ClGSB7.1) associated with GSB resistance, explaining between 6.4 and 21.1% of the phenotypic variation. The genes underlying ClGSB5.1 includes an NBS-LRR gene (ClCG05G019540) previously identified as a candidate gene for GSB resistance in watermelon. Locus ClGSB7.1 accounted for the highest phenotypic variation and harbors twenty-two candidate genes associated with disease resistance. Among them is ClCG07G013230, encoding an Avr9/Cf-9 rapidly elicited disease resistance protein, which contains a non-synonymous point mutation in the DUF761 domain that was significantly associated with GSB resistance. High throughput markers were developed for selection of ClGSB5.1 and ClGSB7.1. Our findings will facilitate the use of molecular markers for efficient introgression of the resistance loci and development of GSB-resistant watermelon cultivars.

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Section: Discussionmentioning

confidence: 95%

Section: Candidate Gene Identificationmentioning

confidence: 99%

Section: Electronic Supplementary Materialsmentioning

confidence: 99%

Section: Electronic Supplementary Materialsmentioning

confidence: 99%

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QTL associated with gummy stem blight resistance in watermelon

et al. 2020

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“…The original files generated from high-throughput sequencing were converted to raw reads by using base calling software Illunima Casava 1.8, the quality evaluation was performed to obtain clean reads as described by Ren et al (2019) [51]. Then, the filtered short reads were aligned to the reference Chinese cabbage genome (http://brassicadb.org/brad/) using the BWA (Burrows-Wheeler-Aligner) software [52].…”

Section: Alignment With the Reference Genome And Variant Callingmentioning

confidence: 99%

Conjunctive Analyses of BSA-Seq and BSR-Seq to Reveal the Molecular Pathway of Leafy Head Formation in Chinese Cabbage

Hou

Gao

et al. 2019

Plants

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As the storage organ of Chinese cabbage, the leafy head was harvested as a commercial product due to its edible value. In this study, the bulked segregant analysis (BSA) and bulked segregant RNA-Seq (BSR) were performed with F 2 separation progeny to study the molecular mechanism of leafy head formation in Chinese cabbage. BSA-Seq analysis located four candidate regions containing 40 candidate genes, while BSR-Seq analysis revealed eight candidate regions containing 607 candidate genes. The conjunctive analyses of these two methods identified that Casein kinase gene BrCKL8 (Bra035974) is the common candidate gene related with leafy head formation in Chinese cabbage, and it showed high expression levels at the three segments of heading type plant leaves. The differentially expressed genes (DEGs) between two set pairs of cDNA sequencing bulks were divided into two categories: one category was related with five hormone pathways (Auxin, Ethylene, Abscisic acid, Jasmonic acid and Gibberellin), the other category was composed of genes that associate with the calcium signaling pathway. Moreover, a series of upregulated transcriptional factors (TFs) were also identified by the association analysis of BSR-Seq analysis. The leafy head development was regulated by various biological processes and effected by diverse external environment factors, so our research will contribute to the breeding of perfect leaf-heading types of Chinese cabbage.

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Translational Genomics of Cucurbit Oil Seeds

McGregor

Meru

2021

Oil Crop Genomics

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Identification and Molecular Mapping of a Gummy Stem Blight Resistance Gene in Wild Watermelon (Citrullus amarus) Germplasm PI 189225

Cited by 29 publications

References 56 publications

QTL associated with gummy stem blight resistance in watermelon

QTL associated with gummy stem blight resistance in watermelon

Conjunctive Analyses of BSA-Seq and BSR-Seq to Reveal the Molecular Pathway of Leafy Head Formation in Chinese Cabbage

Translational Genomics of Cucurbit Oil Seeds

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