Identification and molecular cloning of Atlantic cod (Gadus morhua) activating transcription factor 3 (ATF3) transcript and its induction in spleen following intraperitoneal polyriboinosinic polyribocytidylic acid injection

Feng, Charles Y.; Rise, Matthew L.

doi:10.1016/j.fsi.2011.06.002

Cited by 12 publications

(7 citation statements)

References 29 publications

(49 reference statements)

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“…ATF3 was found to control IFN signalling and to repress PAMP-stimulated cytokine responses in mammalian macrophages [ 112 , 113 ]. Consistent with the present study, Feng and Rise [ 114 ] characterised atf3 as an evolutionarily-conserved and pIC-inducible transcript in Atlantic cod. Although we showed the involvement of cAMP-dependent factors such as atf3 in the antiviral responses of salmon MLCs, the precise functions of these factors in teleost macrophages are yet to be investigated.…”

Section: Discussionsupporting

confidence: 92%

Transcriptome profiling of antiviral immune and dietary fatty acid dependent responses of Atlantic salmon macrophage-like cells

et al. 2017

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BackgroundDue to the limited availability and high cost of fish oil in the face of increasing aquaculture production, there is a need to reduce usage of fish oil in aquafeeds without compromising farm fish health. Therefore, the present study was conducted to determine if different levels of vegetable and fish oils can alter antiviral responses of salmon macrophage-like cells (MLCs). Atlantic salmon (Salmo salar) were fed diets containing 7.4% (FO7) or 5.1% (FO5) fish oil. These diets were designed to be relatively low in EPA + DHA (i.e. FO7: 1.41% and FO5: 1%), but near the requirement level, and resulting in comparable growth. Vegetable oil (i.e. rapeseed oil) was used to balance fish oil in experimental diets. After a 16-week feeding trial, MLCs isolated from fish in these dietary groups were stimulated by a viral mimic (dsRNA: pIC) for 6 h (qPCR assay) and 24 h (microarray and qPCR assays).ResultsThe fatty acid composition of head kidney leukocytes varied between the two dietary groups (e.g. higher 20:5n-3 in the FO7 group). Following microarray assays using a 44K salmonid platform, Rank Products (RP) analysis showed 14 and 54 differentially expressed probes (DEP) (PFP < 0.05) between the two diets in control and pIC groups (FO5 vs. FO7), respectively. Nonetheless, Significance Analysis of Microarrays (SAM, FDR < 0.05) identified only one DEP between pIC groups of the two diets. Moreover, we identified a large number (i.e. 890 DEP in FO7 and 1128 DEP in FO5 overlapping between SAM and RP) of pIC-responsive transcripts, and several of them were involved in TLR−/RLR-dependent and cytokine-mediated pathways. The microarray results were validated as significantly differentially expressed by qPCR assays for 2 out of 9 diet-responsive transcripts and for all of the 35 selected pIC-responsive transcripts.Conclusion Fatty acid-binding protein adipocyte (fabp4) and proteasome subunit beta type-8 (psmb8) were significantly up- and down-regulated, respectively, in the MLCs of fish fed the diet with a lower level of fish oil, suggesting that they are important diet-responsive, immune-related biomarkers for future studies. Although the different levels of dietary fish and vegetable oils involved in this study affected the expression of some transcripts, the immune-related pathways and functions activated by the antiviral response of salmon MLCs in both groups were comparable overall. Moreover, the qPCR revealed transcripts responding early to pIC (e.g. lgp2, map3k8, socs1, dusp5 and cflar) and time-responsive transcripts (e.g. scarb1-a, csf1r, traf5a, cd80 and ctsf) in salmon MLCs. The present study provides a comprehensive picture of the putative molecular pathways (e.g. RLR-, TLR-, MAPK- and IFN-associated pathways) activated by the antiviral response of salmon MLCs.Electronic supplementary materialThe online version of this article (10.1186/s12864-017-4099-2) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 92%

Transcriptome profiling of antiviral immune and dietary fatty acid dependent responses of Atlantic salmon macrophage-like cells

et al. 2017

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“…Among the genes differentially regulated in both soy and wheat diets-fed fish were PDZ and LIM domain 1 (pdlim1, downregulation), involving in actin cytoskeleton organization; serotransferrin (tfa, downregulation), important for ion transport as well as response to bacterium; mutS homolog 3 (msh3, upregulation), vital for DNA repair; and activating transcription factor 3 (atf3, downregulation), needed for regulation of transcription and fish immune responses (Feng and Rise, 2011).…”

Section: Feeding Soy and Wheat Diets Resulted In Regulation Of Genes mentioning

confidence: 99%

Plant-Based Diets Induce Transcriptomic Changes in Muscle of Zebrafish and Atlantic Salmon

et al. 2020

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“…While ATF3 suppressed IFNg expression in mouse natural killer cells during a murine cytomegalovirus (MCMV) infection, it was involved in the transactivation of the IFNg gene during early T-cell differentiation in human [71,73]. However, little is known about ATF3 in fish immune responses, except for a recent study demonstrating that the ATF3 may be involved in the modulation of innate antiviral response in Atlantic cod (G. morhua) [69]. There have not been any reports showing the involvement of ATF3 in skin immune response prior to this study.…”

Section: Discussionmentioning

confidence: 99%

Gene expression profiling in the skin of zebrafish infected with Citrobacter freundii

Lü

Xue

et al. 2012

Fish & Shellfish Immunology

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Identification and molecular cloning of Atlantic cod (Gadus morhua) activating transcription factor 3 (ATF3) transcript and its induction in spleen following intraperitoneal polyriboinosinic polyribocytidylic acid injection

Cited by 12 publications

References 29 publications

Transcriptome profiling of antiviral immune and dietary fatty acid dependent responses of Atlantic salmon macrophage-like cells

Transcriptome profiling of antiviral immune and dietary fatty acid dependent responses of Atlantic salmon macrophage-like cells

Plant-Based Diets Induce Transcriptomic Changes in Muscle of Zebrafish and Atlantic Salmon

Gene expression profiling in the skin of zebrafish infected with Citrobacter freundii

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