Identification and Molecular Characterization of Antibiotics Resistance Staphylococcus species from of the Nasal Cavity of Goats in Adama, Ethiopia

Abstract

Background Staphylococci spp. are found everywhere in nature and they occur as a part of the normal microbial flora. These bacterial spp colonizes the skin, and mucous membranes in the nostrils of humans and animals. The aim of this study was to identify and molecular characterization of antibiotic resistance staphylococcus species that isolated from the nasal cavity of goats (NCGs) in Adama city. Results In this study, 34 isolates of PSSIs were obtained from NCGs. All are morphologically cocci. The recent all PSSIs were shown positive results for catalase, gram staining, and urease test. Few isolates were found to be Coagulase positive and were confirmed to be S. aureus. In this study, PSSIs were identified as S. aureus, S. sciuri, S. simulans, and S. xylosus using MALDI-TOF MS analysis. Antibiotic resistance gene such as blaZ, mecA, and nuc gene were detected for PSSI-D8 strain using PCR analysis. Based on 16srRNA analysis, PSSI-D8 was confirmed to be Staphylococcus aureus with 99.47% sequence similarity and designated as Staphylococcus aureus isolate obtained from NCGs (Staphylococcus aureus INCGD8). PSSI-D8 strain found to be (100%) susceptible to chloramphenicol and vancomycin antibiotics. The optimum condition for growth and biomass production for Staphylococcus aureus INCG8 was obtained at pH7, temperature (37oC) and 1.28M of NaCl using glucose as the main carbon source. Conclusion In conclusion, the preliminary, secondary screening, MALDI-TOF MS analysis, and molecular study collectively were driving the PSSI-D8 to strain of S. aureus for the first time in Ethiopia from NCGs.