Identification and molecular characterization of Van A-type vancomycin-resistant Enterococcus faecalis in Northeast of Brazil

Vilela, Marinalda Anselmo; Souza, S. Lavareda De; Palazzo, Izabel Cristina Vanzato; Ferreira, Joseane Cristina; Morais, Marcos Antônio de; Darini, Ana Lúcia da Costa; Morais, Márcia Maria Camargo de

doi:10.1590/s0074-02762006000700002

Cited by 10 publications

(8 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These percentages of resistance are significant, while lower or occasionally comparable to those previously reported for enterococci recovered from human sources in Brazil (Mondino et al 2003, d'Azevedo et al 2004 ) and other locations (Lopes et al 2003). The observation of concomitant resistance to chloramphenicol, erythromycin, and tetracycline confirms the frequent resistance to multiple antibiotics among enterococcal isolates from humans sources in Brazil (Mondino et al 2003, Vilela et al 2006. Although a large proportion of (Bersos et al 2004).…”

Section: Discussionsupporting

confidence: 75%

Antimicrobial resistance profiles of enterococci isolated from poultry meat and pasteurized milk in Rio de Janeiro, Brazil

Fracalanzza

Scheidegger

Santos

et al. 2007

Mem. Inst. Oswaldo Cruz

View full text Add to dashboard Cite

show abstract

Section: Discussionsupporting

confidence: 75%

Antimicrobial resistance profiles of enterococci isolated from poultry meat and pasteurized milk in Rio de Janeiro, Brazil

Fracalanzza

Scheidegger

Santos

et al. 2007

Mem. Inst. Oswaldo Cruz

View full text Add to dashboard Cite

show abstract

“…In previous studies, vanA plasmid of 70 kb was found in both species of VRE from hospitals of several states in Brazil, suggesting horizontal transfer, what differs from the results of this study. 25,35 Regarding the plasmid family content, we checked whether each species had different rep genes or not. This was the first report of rep-genes of VRE plasmids from Brazil.…”

Section: Discussionmentioning

confidence: 99%

Different VanA Elements inE. faecalisand inE. faeciumSuggest at Least Two Origins of Tn1546Among VRE in a Brazilian Hospital

Merlo

Dabul

Camargo

2015

Microbial Drug Resistance

View full text Add to dashboard Cite

In 2009 during surveillance in a Brazilian hospital, many patients were confirmed to be colonized by vancomycin-resistant enterococci (VRE) and a few infection cases occurred. Among 14 isolates of Enterococcus faecalis, most had the same pulsotype, virulence profile (agg(+)elrA(+)gelE(+)), and were sequence type (ST)103, indicating dissemination of a clone. The 47 Enterococcus faecium were separated into four pulsotypes, the predominant virulence profile being esp(+)acm(+). All of them harbored the hospital marker IS16, and three randomly chosen isolates were ST412, belonging to the Clonal Complex 17. E. faecalis were all susceptible to penicillin and ampicillin, while all E. faecium were resistant to them. All isolates were susceptible to daptomycin and tigecycline. There were no rep-family genes common to all VRE. The VanA element of all E. faecium lost its left-side inverted repeat (IRL) region and had a specific IS insertion. On the other hand, all E. faecalis presented intact Tn1546. The size of plasmids containing the vanA gene as well as its rep-families varied between and within species. The lack of a vanA plasmid common to all VRE, together with the differences among VanA elements, despite the fact that some patients were colonized by both species during their hospitalization, leads us to suggest at least two different Tn1546 origins.

show abstract

“…The enterococus suspicious colonies were confi rmed genetically using genus [15] and species [16] specifi c primers for the PCR. The Uniplex PCR was used for the detection of antibiotic resistance genes in resistance strains.…”

Section: Molecular Methodsmentioning

confidence: 99%

Distribution of Antibiotic Resistance Genes in Enterococcus spp. Isolated from Mastitis Bovine Milk

Erbaş¹,

Parın²,

Türkyılmaz³

et al. 2016

Acta Veterinaria

View full text Add to dashboard Cite

In this study, determination of enterococcus species that were isolated from mastitic milk samples, investigation of their susceptibilities to antibiotics and identifi cation of the existence of resistance genes in resistant strains were conducted. The specimens consist of 600 mastitic milk samples that were collected from 242 cows. Isolation of enterococcus was carried out in selective media and 94 (15.6%) Enterococcus spp. were isolated. A total of 94 species of Enterococci were identifi ed using both sequencing and polymerase chain reaction (PCR). Enterococcus spp. isolates belong to 5 different species (E. faecalis, E. faecium, E. durans, E. hirae, E. mundtii) in sequence analysis and 4 different species (E. faecalis, E. faecium, E. durans, E. hirae) were identify by PCR method with specifi c primers. Analyzing 94 enterococcus strains by antibiotic sensitiveness test a high rate of resistance to tetracycline in 77 (81.9%) isolates was shown. The tet resistance genes were identifi ed as follows: 54 were tetM positive, 23 were tetK positive and 17 were positive on tetM and tetK. Resistance to erythromycin was established in 27 (28.7%) isolates (25 ermB) while the chloramphenicol resistance gene was found in 10 (10.7%) of isolates and the cat gene was identifi ed in nine samples and one isolate was resistant to vancomycin (1.06%) with the VanA gene confi rmed. In conclusion, it was shown that E. faecalis has the biggest role in enterococcus originated mastitis and these strains were found to be mostly resistant to tetracycline. One vancomycine resistant isolate that had the VanA gene was also determined.

show abstract

Identification and molecular characterization of Van A-type vancomycin-resistant Enterococcus faecalis in Northeast of Brazil

Abstract: The isolation of vancomycin resistant enterococci (VRE)

Cited by 10 publications

References 27 publications

Antimicrobial resistance profiles of enterococci isolated from poultry meat and pasteurized milk in Rio de Janeiro, Brazil

Antimicrobial resistance profiles of enterococci isolated from poultry meat and pasteurized milk in Rio de Janeiro, Brazil

Different VanA Elements inE. faecalisand inE. faeciumSuggest at Least Two Origins of Tn1546Among VRE in a Brazilian Hospital

Distribution of Antibiotic Resistance Genes in Enterococcus spp. Isolated from Mastitis Bovine Milk

Contact Info

Product

Resources

About