Identification and Molecular Characterisation of a Novel Manganese Superoxide Dismutase Gene from Flesh Fly Larvae,<i>Sarcophaga argyrostoma</i>(Diptera: Sarcophagidae)

Mahmoud, S. A.; Moselhy, Walaa A.; El-Khashab, L.A.A.; Seufi, AlaaEddeen M.

doi:10.4001/003.026.0448

Cited by 1 publication

(2 citation statements)

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“…Hemolymph (1 µL/larva) was pooled by cutting off the anterior tip of the larvae with sterile fine scissors in a prechilled eppendorf containing few crystals of phenylthiourea to prevent melanization [ 42 ]. Hemolymph samples were collected at 6, 12 and 24 h post-injection and from untreated larvae as controls.…”

Section: Methodsmentioning

confidence: 99%

“…Therefore, bioactive peptides with beneficial biological activity [ 35 ] such as anti-inflammatory, antioxidant, antihypertensive and even hypocholesterolemic can be a good source of nutritional value [ 36 , 37 , 38 , 39 ] via stimulation of GST and CAT antioxidant enzymes and DPPH radical scavenging activities such as Gryllodes sigillatus hydrolysates [ 35 ] and the aqueous extract of Calliptamus italicus , Bombyx mori , Vespa affinis and Acheta domesticus [ 40 , 41 ]. A series of investigations confirmed the potential of several chemicals; H 2 O 2 [ 42 ] and biological compounds [ 43 ] including pesticides and prooxidant allelochemicals to elicit oxidative stress (OS) conditions in the insect models [ 44 ], posing a serious challenge to the insect species.…”

Section: Introductionmentioning

confidence: 99%

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Enhanced Antioxidant and Cytotoxic Potentials of Lipopolysaccharides-Injected Musca domestica Larvae

et al. 2020

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The usage of insects as a sustainable and functional natural products resource is a new promise in complementary and alternative medicine. The present study aimed to investigate the ability of Musca domestica (housefly) larval hemolymph (insect blood) to display the enhanced in vitro antioxidant and cytotoxic effects. The oxidative stress (OS) was elicited by inducing lipopolysaccharides (LPS) treatment as an exogenous stressor. Determination of superoxide dismutase 1 (SOD1), glutathione (GSH), malondialdehyde (MDA) and total antioxidant capacity (TAC), and mRNA and protein expressions of SOD1, was investigated as confirmatory markers of oxidative stress induction. Cytotoxicity on cancerous MCF-7 and normal Vero cells were also evaluated using an MTT assay at 24 h post-injection. The injection of LPS induced a significant (p < 0.05) increase in SOD, GSH and TAC, whereas, the MDA was diminished. Hemolymph was collected from normal and treated larvae after 6, 12 and 24 h. The M. domestica superoxide dismutase (MdSOD1) transcripts were significantly (p < 0.05) upregulated 6 and 12 h post-treatment, while a significant downregulation was observed after 24 h. Western blot analysis showed that MdSOD1 was expressed in the hemolymph of the treated larvae with an increase of 1.2 folds at 6 and 12 h and 1.6 folds at 24 h relative to the control group. LPS-treated larval hemolymphs exhibited significant cytotoxicity with respect to the untreated ones against MCF-7 while Vero cells showed no cytotoxicity for both hemolymphs. The DPPH free radical scavenging activity was examined and a significant antioxidant potential potency was observed at 6 h (50% maximal inhibitory concentration (IC50): 63.3 ± 3.51 µg/mL) when compared to the control M. domestica larval hemolymph (IC50: 611.7 ± 10.41 µg/mL). Taken together, M. domestica larval hemolymph exhibited enhanced antioxidant and consequently increased cytotoxic capacities under stressed conditions.

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Section: Methodsmentioning

confidence: 99%