Identification and Minimization of Nonideal Binding Effects in BIAcore Analysis: Ferritin/Anti-ferritin Fab′ Interaction as a Model System

Direct kinetic assay of interactions between small peptides and immobilized antibodies using a surface plasmon resonance biosensor

“…1999 for interaction analysis has made it possible to obtain affinity and kinetic data for a large Ž number of antigen-antibody Brigham-Burke et al, 1992;VanCott et al, 1994;Oddie et al, 1997;. England et al, 1997;Houshmand et al, 1999, Ž .…”

Direct single-step surface plasmon resonance analysis of interactions between small peptides and immobilized monoclonal antibodies

“…cessful analysis: i high flow rates and ii high peptide concentrations to avoid mass-transport limi-Ž . tations, and iii medium-density mAb surfaces to Ž minimize ligand site heterogeneity O' Shannessy and Winzor, 1996;Myszka et al, 1997;Oddie et al, . 1997 .…”

Analysis of the Binding of the Fab Fragment of Monoclonal Antibody NC10 to Influenza Virus N9 Neuraminidase from Tern and Whale Using the BIAcore Biosensor: Effect of Immobilization Level and Flow Rate on Kinetic Analysis

“…specific amine immobilization procedure (33). These problems can be overcome, or at least minimized, by experimental design and site-specific immobilization of the ligand (26,27,34). Multivalent binding problems can be avoided by immobilization of the multivalent molecule and employing a monovalent form of the analyte for kinetic measurements.…”

“…The theory of kinetic measurement for an antigen-antibody interaction, where one reactant is immobilized on a sensor surface as in the BIAcore biosensor, has been extensively reviewed (18 -25). The evaluation of association (k a ) and dissociation (k d ) rate constants was performed by applying linear or nonlinear fitting algorithms to the binding data using the 1:1 Langmuir binding model (19,26,27). The data were also analyzed using global fitting procedures in the BIAevaluation 3.0 program (BIAcore) applying the 1:1 Langmuir binding model using rate equations with and without the term for mass transport (25,28).…”