Identification and metabolite profiling ofSitophilus oryzaeL. by 1D and 2D NMR Spectroscopy

Abstract

The polyphagous insect Sitophilus oryzae L. (Coleoptera:Curculionidae) has a tremendous adaptability in feeding behaviour, making it a serious invasive pest of stored cereals. The present study identifies the metabolite composition of Sitophilus oryzae (S. oryzae) using Nuclear Magnetic Resonance (NMR) spectroscopy. Assignment of 1D-proton by NMR, 1H-1H COSY, 2D-TOCSY 1H-1H, had been done. Amongst the various biochemically important metabolites isoleucine, valine, leucine, beta-hydroxybutyrate, lysine, glutamate, glutamine, proline, lactate, alanine, di-methylamine, alpha-glucose, beta-glucose, choline, glycerophosphorylcholine and tyrosine are present in S. oryzae. In wheat-fed S. oryzae, the presence of threonine and the absence of lactate is observed. In rice-fed S. oryzae, however, the presence of lactate and the absence of threonine were observed. Barley-fed S. oryzae shows presence of both tyrosine and lactate. It is concluded that the pest S. oryzae has adaptability on different stored cereals and grains, depicting the presence of earlier reported metabolites. The present study aims to identify the key metabolic components and associated enzymes in Sitophilus oryzae fed on different cereals.

Biopesticidal Potential of Indigenous Plant Extracts against Rice Weevil in Stored Wheat

“…Among these pests, rice weevil (Sitophilus oryzae), with its ability to infest a wide range of grains, stands out as a significant contributor to the overall challenge of managing stored grain pests on a global scale Parisot et al, 2021). Sitophilus oryzae emerges as a major threat in stored grains, particularly wheat, due to its rapid reproductive cycle and adaptability (Trivedi et al, 2010;Zunjare et al, 2015;Cao et al, 2024). Its capacity to infest a variety of grains amplifies the economic losses associated with stored grain pests, making S. oryzae a crucial target for effective pest management strategies (Togola et al, 2013;Yaseen et al, 2019).…”