Identification and measurement of β-endorphin levels in the skin during induced hair growth in mice

Furkert, Jens; Klug, U.; Slominski, Andrzej; Eichmüller, Stefan B.; Mehlis, B.; Kertscher, U.; Paus, Ralf

doi:10.1016/s0304-4165(97)00046-9

Cited by 26 publications

(37 citation statements)

References 36 publications

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“…The results reported here can also be directly correlated with an earlier biochemical and molecular biology study in which we had demonstrated by RT-PCR that POMC mRNA is expressed constitutively in murine skin . In addition, we have also shown that POMC mRNA is upregulated during anagen development and that this is accompanied by increased production of ␤ -endorphin and ACTH (Slominski et al 1992Furkert et al 1997). …”

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confidence: 82%

Spatiotemporal Expression, Distribution, and Processing of POMC and POMC-derived Peptides in Murine Skin

Mazurkiewicz

Corliss

Słomiński

2000

J Histochem Cytochem.

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S U M M A R YIn murine skin, after depilation-induced anagen, there was a differential spatial and temporal expression of pro-opiomelanocortin (POMC) mRNA, of the POMC-derived peptides ␤ -endorphin, ACTH, ␤ -MSH, and ␣ -MSH, and of the prohormone convertases PC1 and PC2 in epidermal and hair follicle keratinocytes and in the cells of sebaceous units. Using a combination of in situ hybridization histochemistry and immunohistochemistry, we found cell-specific variations in the expression of POMC mRNA that were consistent with immunoreactivities for POMC-derived peptides. Cells that contained POMC peptide immunoreactivity (IR) also expressed POMC mRNA, and where the IR increased there was a parallel increase in mRNA. The levels of PC1-IR and PC2-IR also showed cell-specific variations and were present in the same cells that contained the POMC peptides. Based on the cleavage specificities of these convertases and on the spatial and temporal expression of the convertases and of ACTH, ␤ -endorphin, ␤ -MSH, and ␣ -MSH, we can infer that the activities of PC1 and PC2 are responsible for the cell-specific differential processing of POMC in murine skin.

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confidence: 82%

Spatiotemporal Expression, Distribution, and Processing of POMC and POMC-derived Peptides in Murine Skin

Mazurkiewicz

Corliss

Słomiński

2000

J Histochem Cytochem.

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“…The analytical method of reversed-phase high performance liquid chromatography (RP-HPLC) was used to document production of ACTH and a-MSH by keratinocytes and melanocytes (4,11). Recent immunocytochemical, western blot and radioimmunoassay analyses showed presence of b-endorphin-like immunoreactivity in the skin (2,3,(12)(13)(14). Although b-endorphin peptide was identified by RP-HPLC in full thickness mouse skin (14), its production by isolated skin cells using similar analytical methods re-mained to be provided.…”

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confidence: 99%

“…Recent immunocytochemical, western blot and radioimmunoassay analyses showed presence of b-endorphin-like immunoreactivity in the skin (2,3,(12)(13)(14). Although b-endorphin peptide was identified by RP-HPLC in full thickness mouse skin (14), its production by isolated skin cells using similar analytical methods re-mained to be provided. Therefore, we have undertaken an effort to identify b-endorphin by RP-HPLC in cultured human melanocytes, melanoma and squamous cells carcinoma cells.…”

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confidence: 99%

“…The eluted fractions after lyophilization were suspended in 0.1% trifluoroacetic acid (TFA) and fractionated by RP-HPLC using Beckam Ultrashere C18 IP column (14)(15)(16). A linear gradient of 20 to 47% acetonitrile in 0.1% TFA with flow rate 1 ml/min (1 ml/fraction) was used to elute peptides (14). The RP-HPLC separations were performed by James Baker (Albany Medical College).…”

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confidence: 99%

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Identification of β‐endorphin, α‐MSH and ACTH peptides in cultured human melanocytes, melanoma and squamous cell carcinoma cells by RP‐HPLC

Słomiński

1998

Experimental Dermatology

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“…c-MSH plays a role in cardiac function and is natriuretic due to an interaction with renal nerves (Chen et al 1997). b-EP is expressed in skin and plays a role in the cyclic change of hair growth (Furkert et al 1997). It inhibits the activity of the gonadotropin-releasing hormone and the thyrotropinreleasing hormone (Sánchez-Franco and Cacicedo 1986).…”

Section: Introductionmentioning

confidence: 99%

cDNA cloning, pituitary location, and extra-pituitary expression of pro-opiomelanocortin gene in rare minnow (Gobiocypris rarus)

Liu

Xie

Zhang

et al. 2010

Fish Physiol Biochem

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A cDNA encoding pro-opiomelanocortin (POMC) gene was cloned from the pituitary gland of the rare minnow (Gobiocypris rarus), a small freshwater fish endemic to China. This was achieved by reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE). Data showed that the predicted rare minnow POMC (rmPOMC) cDNA consisted of 846bps coding for the following sequences, flanked by proteolytic cleavage sites: signal peptide (SP, Met(1)-Ala(28)), N-terminal peptide (Gln(29)-His(105)), ACTH (Ser(108)-Met(146)), α-MSH (Ser(108)-Gal(121)), CLIP (Pro(126)-Met(146)), β-LPH (Glu(149)-His(221)), γ-LPH (Glu1(49)-Ser(186)), β-MSH (Asp(170)-Ser(186)), and β-endorphin (β-EP, Tyr(189)-Gln(221)). Sequence analysis showed no region was homologous to γ-MSH (a tetrapod POMC feature). The amino acid sequence is highly similar to POMC-I and POMC-II of the common carp (92.4%), according to homologous alignment. It was POMCα through the phylogenetic analysis. Pituitary and extra-pituitary expression were studied using RT-PCR and in situ hybridization. The rmPOMC-positive cells were mainly located in the rostral pars distalis (RPD) and pars intermedia (PI). Some rmPOMC-positive cells were detected in the proximal pars distalis (PPD) as well, according to in situ hybridization. In the extra-pituitary tissues, positive signals were observed in the brain, intestines, gonads, hepatopancreas, spleen, and gills by RT-PCR analysis.

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Identification and measurement of β-endorphin levels in the skin during induced hair growth in mice

Cited by 26 publications

References 36 publications

Spatiotemporal Expression, Distribution, and Processing of POMC and POMC-derived Peptides in Murine Skin

Spatiotemporal Expression, Distribution, and Processing of POMC and POMC-derived Peptides in Murine Skin

Identification of β‐endorphin, α‐MSH and ACTH peptides in cultured human melanocytes, melanoma and squamous cell carcinoma cells by RP‐HPLC

cDNA cloning, pituitary location, and extra-pituitary expression of pro-opiomelanocortin gene in rare minnow (Gobiocypris rarus)

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