Identification and Manipulation of Antigen Specific T-Cells with Artificial Antigen Presenting Cells

Koffeman, Eva; Keogh, Elissa; Klein, Mark; Prakken, Berent J.; Albani, Salvatore

doi:10.1007/978-1-59745-402-5_6

Cited by 7 publications

(5 citation statements)

References 21 publications

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“…T cells are involved in cell-mediated immunity. They are essential in determining B cell antibody class switching, in the activation and growth of cytotoxic T cells, and in maximizing bactericidal activity of phagocytes such as macrophages (Koffeman et al 2007). It is probably this diversity in function that makes the T cell subgroup of lymphocytes a target for destruction by T. solium cysticerci.…”

Section: Discussionmentioning

confidence: 99%

Host-cell apoptosis inTaenia solium-induced brain granulomas in naturally infected pigs

et al. 2008

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SUMMARYTo assess whether apoptosis occurs in pig brain granulomas due toTaenia soliumcysticerci, brain tissues from 30 pigs naturally infected withT. soliumcysticercosis were evaluated by terminal deoxynucleotidyl transferase-end labelling (TUNEL) staining. In addition, tissues were stained with CD3 marker to identify T lymphocytes. Examination of TUNEL-stained tissues showed apoptotic cells in early lesions that contained viable cysticerci. Apoptotic cells were primarily found interspersed with normal cell types, and were mostly located in the inflammatory infiltrate. Late or advanced granulomas with disintegrated scolices did not show TUNEL-positive cells. CD3+ cells were found in both early and advanced lesions and apoptosis mainly co-localized with CD3+ T lymphocytes. This suggests that these cells are constantly undergoing apoptosis and thus die as soon as they arrive at the site of infection. Apoptosis indeed may be one way by whichT. soliumcysticerci down-regulate the host's cellular immune response in early cysticercosis. Therefore, further research is needed to establish if other cells besides T-lymphocytes are also a target for destruction by cysticerci in early cysticercosis as well as studies to assess if cysteine protease is expressed by viable cysticerciin situ.

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Section: Discussionmentioning

confidence: 99%

Host-cell apoptosis inTaenia solium-induced brain granulomas in naturally infected pigs

et al. 2008

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“…T-cell maturation and activation phenotype were evaluated by staining with the following mouse-anti-human mAb in different combinations: FITClabeled anti-CD3, PE-labeled anti-CD25 (Miltenyi Biotech, Gladbach, Germany), FITC-labeled anti-CD62L, FITC-labeled CD27, PE-labeled anti-CD45RA, PE-or PerCP-labeled anti-CD4, anti-CD69 and anti-CD3, APClabeled anti-CD8 and anti-CD4 (BD Biosciences, San Jose, CA, USA), APC labeled anti-CCR7 (R&D systems, Minneapolis, MN, USA). To detect antigen-specific T cells directed to Melan-A/MART-1, lymphocytes were stained with PE-labeled tetramers of HLA-A 0201 containing Melan-A/MART-1 [27][28][29][30][31][32][33][34][35] peptide (Beckman Coulter Inc., Fullerton, CA, USA). Surface staining was performed by incubating mAb at 4°C for 30 min.…”

Section: Flow Cytometry Analysismentioning

confidence: 99%

“…29,30 In fact, these artificial APC lack a fluid membrane, which would allow ligands to cluster and activate T cells efficiently. 31 The aim of this study was to engineer an artificial APCbased system with the properties of a fluid cellular membrane and the flexibility derived from an artificial structure that could be tailored to carry the desired immunostimulatory molecules. Recent evidence indicated that preclustering of MHC-peptide complexes in membrane microdomains on the APC surface affects the efficiency of immune synapse formation and the related T-cell activation.…”

Section: Introductionmentioning

confidence: 99%

The effect of artificial antigen-presenting cells with preclustered anti-CD28/-CD3/-LFA-1 monoclonal antibodies on the induction of ex vivo expansion of functional human antitumor T cells

Zappasodi¹,

Nicola²,

Carlo‐Stella³

et al. 2008

Haematologica

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BackgroundAdoptive cell therapy with ex vivo expanded autologous antitumor cytotoxic T lymphocytes represents an important therapeutic option as an anticancer strategy. In order to identify a reliable method for producing adequate amounts of functional antitumor cytotoxic T lymphocytes with a potentially long in vivo lifespan, we tested the T-cell expansion efficiency of a new artificial antigen-presenting cell-based system.

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“…In general, for rigid spherical particles the number of ligands available to participate in the required simultaneous interaction is limited by its topology. [17][18][19] Second-generation immunotherapeutic delivery vehicles have, therefore, explored extended aspect ratio topologies such as lamentous or rod-like particles. These extended aspect ratio structures have utilized either copolymer lomicelles 20 or decorated carbon nanotubes (CNTs).…”

Section: Introductionmentioning

confidence: 99%

Therapeutic nanoworms: towards novel synthetic dendritic cells for immunotherapy

et al. 2013

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A new class of antibody-functionalized, semi-flexible and filamentous polymers (diameter 5-10 nm, length $200 nm) with a controlled persistence length, a high degree of stereoregularity and the potential for multiple simultaneous receptor interactions has been developed. We have decorated these highly controlled, semi-stiff polymers with T cell activating anti-CD3 antibodies and analyzed their application potential as simple synthetic mimics of dendritic cells (sDCs). Our sDCs do not only activate T cells at significantly lower concentrations than free antibodies or rigid sphere-like counterparts (PLGA particles) but also induce a more robust T cell response. Our novel design further yields sDCs that are biocompatible and non-toxic. The observed increased efficacy highlights the importance of architectural flexibility and multivalency for modulating T cell response and cellular function in general.

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Identification and Manipulation of Antigen Specific T-Cells with Artificial Antigen Presenting Cells

Cited by 7 publications

References 21 publications

Host-cell apoptosis inTaenia solium-induced brain granulomas in naturally infected pigs

Host-cell apoptosis inTaenia solium-induced brain granulomas in naturally infected pigs

The effect of artificial antigen-presenting cells with preclustered anti-CD28/-CD3/-LFA-1 monoclonal antibodies on the induction of ex vivo expansion of functional human antitumor T cells

Therapeutic nanoworms: towards novel synthetic dendritic cells for immunotherapy

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