Identification and localization of ryanodine binding proteins in the avian central nervous system

Ellisman, Mark H.; Deerinck, Thomas J.; Ouyang, Yanling; Beck, Claudia F.; Tanksley, Steven J.; Walton, Philip D.; Airey, Judith A.; Sutko, John L.

doi:10.1016/0896-6273(90)90304-x

Cited by 144 publications

(91 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These structures appear therefore equipped to sustain rapid Ca>2 exchange and may thus correspond to the two distinct ER subcompartments, one sensitive and the other insensitive to IP3. Dissociation of the two receptor-channels was observed also in the spines, the expansions of dendrites where impinging synapses are addressed (Ellisman et al, 1990). Recent data on sea urchin eggs have shown the RyR to be concentrated in superficial vesicles and cisternae and excluded from the rest of the ER (McPherson et al, 1992).…”

Section: Ca2+ Storesmentioning

confidence: 92%

Endoplasmic reticulum: a dynamic patchwork of specialized subregions.

Sitia

Meldolesi

1992

MBoC

150

View full text Add to dashboard Cite

Section: Ca2+ Storesmentioning

confidence: 92%

Endoplasmic reticulum: a dynamic patchwork of specialized subregions.

Sitia

Meldolesi

1992

MBoC

150

View full text Add to dashboard Cite

“…It is also possible that at least some of the dense projections represent the ryanodine receptor itself, because the ryanodine receptor has been detected at high concentration in PCs (see below) (Ellisman et al, 1990;Walton et al, 1991). However, cistemal stacks were not described in the immunocytochemical studies of Ellisman et al (1990) and Walton et al (199 1).…”

Section: Discussionmentioning

confidence: 99%

“…The ryanodine receptor (Ellisman et al, 1990;McPherson and Campbell, 1990), vacuolar Ca2+-ATPases (Gunteski-Hamblin et al, 1988;Lytton and MacLennan, 1988;Kaprielian et al, 1989;Plessers et al, 1991) as well as calsequestrin (Volpe et al, 1990a) or proteins functionally analogous to calsequestrin (Booth and Koch, 1989;Fliegel et al, 1989;Treves et al, 1990) have been identified in nonmuscle cells. In addition, the Ca*+ channel gated by the second messenger inositol 1,4,5 trisphosphate (InsP,), the InsP, receptor, has been identified (Supattapone et al, 1988) and thoroughly characterized (Furuichi et al, 1989;Mignery et al, 1989;Mignery and Siidhof, 1990).…”

mentioning

confidence: 99%

“…They demonstrated that both the InsP, receptor and the ryanodine receptor are localized in the ER, further confirming a central role of the ER in Ca*+ regulation. However, the distribution of the two proteins within the ER appeared to be heterogeneous and different (Mignery et al, 1989;Ellisman et al, 1990;Satoh et al, 1990;Walton et al, 1991). A further investigation of intracellular Ca *+ stores is therefore important and timely.…”

mentioning

confidence: 99%

“…The molecular characterization of two intracellular second messenger-gated Ca*+ channels, the ryanodine receptor (Fleischer and Inui, 1989;Takeshima et al, 1989;Zorzato et al, 1990) and the InsP, receptor (Supattapone et al, 1988;Furuichi et al, 1989;Mignery et al, 1989;Mignery and Siidhof, 1990) and the availability of antibodies specific for these proteins made possible the further investigation of the nature of second messenger-responsive intracellular Ca*+ stores in nonmuscle cells (Mignery et al, 1989;Ross et al, 1989;Ellisman et al, 1990;Otsu et al, 1990;Satoh et al, 1990;Walton et al, 1991). These studies have focused on Purkinje cells (PCs) of the cerebellum because PCs contain particularly high concentrations of both Ca2+ channels.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Ca2+ stores in Purkinje neurons: endoplasmic reticulum subcompartments demonstrated by the heterogeneous distribution of the InsP3 receptor, Ca(2+)-ATPase, and calsequestrin

et al. 1992

View full text Add to dashboard Cite

The nature of second messenger-responsive intracellular Ca2+ stores in neurons remains open for discussion. Here, we demonstrate the existence in Purkinje cells (PCs) of endoplastic reticulum (ER) subcompartments characterized by an uneven distribution of three proteins involved in Ca2+ storage and release: the inositol 1,4,5-trisphosphate (InsP3) receptor, Ca(2+)-ATPase, and calsequestrin. Ca(2+)-ATPase and the InsP3 receptor have a widespread, although not identical, distribution throughout the ER. Calsequestrin is localized throughout the smooth ER and is particularly concentrated in pleiomorphic vesicles with a moderately electron-dense core, which appear to represent a subcompartment of the smooth ER. In double-labeling experiments many of these vesicles were unlabeled by InsP3 receptor antibodies. These results suggest a key role of the ER as an intracellular Ca2+ store and demonstrate a possible structural basis for distinct intracellular Ca2+ pools regulated by different second messengers.

show abstract

Neuromuscular development in the avian paralytic mutant crooked neck dwarf (cn/cn): further evidence for the role of neuromuscular activity in motoneuron survival

et al. 1997

View full text Add to dashboard Cite

Neuromuscular transmission and muscle activity during early stages of embryonic development are known to influence the differentiation and survival of motoneurons and to affect interactions with their muscle targets. We have examined neuromuscular development in an avian genetic mutant, crooked neck dwarf (cn/cn), in which a major phenotype is the chronic absence of the spontaneous, neurally mediated movements (motility) that are characteristic of avian and other vertebrate embryos and fetuses. The primary genetic defect in cn/cn embryos responsible for the absence of motility appears to be the lack of excitation-contraction coupling. Although motility in mutant embryos is absent from the onset of activity on embryonic days (E) 3-4, muscle differentiation appears histologically normal up to about E8. After E8, however, previously separate muscles fuse or coalesce secondarily, and myotubes exhibit a progressive series of histological and ultrastructural degenerative changes, including disarrayed myofibrils, dilated sarcoplasmic vesicles, nuclear membrane blebbing, mitochondrial swelling, nuclear inclusions, and absence of junctional end feet. Mutant muscle cells do not develop beyond the myotube stage, and by E18-E20 most muscles have almost completely degenerated. Prior to their breakdown and degeneration, mutant muscles are innervated and synaptic contacts are established. In fact, quantitative analysis indicates that, prior to the onset of muscle degeneration, mutant muscles are hyperinnervated. There is increased branching of motoneuron axons and an increased number of synaptic contacts in the mutant muscle on E8. Naturally occurring cell death of limb-innervating motoneurons is also significantly reduced in cn/cn embryos. Mutant embryos have 30-40% more motoneurons in the brachial and lumbar spinal cord by the end of the normal period of cell death. Electrophysiological recordings (electromyographic and direct records form muscle nerves) failed to detect any differences in the activity of control vs. mutant embryos despite the absence of muscular contractile activity in the mutant embryos. The alpha-ryanodine receptor that is genetically abnormal in homozygote cn/cn embryos is not normally expressed in the spinal cord. Taken together, these data argue against the possibility that the mutant phenotype described here is caused by the perturbation of a central nervous system (CNS)-expressed alpha-ryanodine receptor. The hyperinnervation of skeletal muscle and the reduction of motoneuron death that are observed in cn/cn embryos also occur in genetically paralyzed mouse embryos and in pharmacologically paralyzed avian and rat embryos. Because a primary common feature in all three of these models is the absence of muscle activity, it seems likely that the peripheral excitation of muscle by motoneurons during normal development is a major factor in regulating retrograde muscle-derived (or muscle-associated) signals that control motoneuron differentiation and survival.

show abstract

Identification and localization of ryanodine binding proteins in the avian central nervous system

Cited by 144 publications

References 47 publications

Endoplasmic reticulum: a dynamic patchwork of specialized subregions.

Endoplasmic reticulum: a dynamic patchwork of specialized subregions.

Ca2+ stores in Purkinje neurons: endoplasmic reticulum subcompartments demonstrated by the heterogeneous distribution of the InsP3 receptor, Ca(2+)-ATPase, and calsequestrin

Neuromuscular development in the avian paralytic mutant crooked neck dwarf (cn/cn): further evidence for the role of neuromuscular activity in motoneuron survival

Contact Info

Product

Resources

About