Identification and localization of major soluble vitreous proteins in human ocular tissue*1

Wu, Chonggang; Sauter, Jennifer L.; Johnson, Pamela Jo; CHEN, C; Olsen, Tyler

doi:10.1016/s0002-9394(03)01416-8

Cited by 3 publications

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“…All types of crystallins found in this study were significantly lower in the vitreous humour from PDR patients compared with that from the control subjects, and a previous study has reported that beta-crystallin B2 was identified by MALDI-TOF in normal vitreous [12]. We do not know why the crystallin levels are decreased in the vitreous of diabetic patients with PDR; the biological functions of crystallins are not completely understood.…”

Section: Discussioncontrasting

confidence: 59%

Characterisation of the vitreous proteome in proliferative diabetic retinopathy

Wang

Hu³

et al. 2012

Proteome Sci

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BackgroundDiabetes can lead to serious microvascular complications such as proliferative diabetic retinopathy (PDR), which results in severe vision loss. The diabetes-induced alterations in the vitreous protein composition in diabetic patients with PDR may be responsible for the presence of PDR. The vitreous humour can be utilised in a variety of studies aimed toward the discovery of new targets for the treatment or prevention of PDR and the identification of novel disease mechanisms. The aim of this study was to compare the protein profile of vitreous humour from diabetic patients with PDR with that of vitreous humour from normal human eyes donated for corneal transplant.ResultsVitreous humour from type 2 diabetic patients with PDR (n = 10) and from normal human eyes donated for corneal transplant (n = 10) were studied. The comparative proteomic analysis was performed using two-dimensional fluorescence difference gel electrophoresis (2-D DIGE). Differentially produced proteins (abundance ratio > 2 or < -2, p < 0.01) were identified by matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF MS) and MALDI-TOF tandem mass spectrometry. A total of 1242 protein spots were detected on the 2-D master gel of the samples, and 57 spots that exhibited statistically significant variations were successfully identified. The spots corresponded to peptide fragments of 29 proteins, including 8 proteins that increased and 21 proteins that decreased in PDR. Excluding the serum proteins from minor vitreous haemorrhage, 19 proteins were found to be differentially produced in PDR patients compared with normal subjects; 6 of these proteins have never been reported to be differentially expressed in PDR vitreous: N(G),N(G)-dimethylarginine dimethylaminohydrolase 1 (DDAH 1), tubulin alpha-1B chain, gamma-enolase, cytosolic acyl coenzyme A thioester hydrolase, malate dehydrogenase and phosphatidylethanolamine-binding protein 1 (PEBP 1). The differential production of pigment epithelium-derived factor (PEDF) and clusterin was confirmed by Western blot analysis.ConclusionsThese data provide an in-depth analysis of the human vitreous proteome and reveal protein alterations that are possibly involved in the pathogenesis of PDR. Further investigation of these special proteins may provide potential new targets for the treatment or the prevention of PDR.

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Section: Discussioncontrasting

confidence: 59%

Characterisation of the vitreous proteome in proliferative diabetic retinopathy

Wang

Hu³

et al. 2012

Proteome Sci

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Diet-induced obesity leads to pro-inflammatory alterations to the vitreous humour of the eye in a rat model

et al. 2017

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Application of proteomic technology in eye research: a mini review

Lam

Chun

et al. 2008

Clinical and Experimental Optometry

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Proteomics is a rapidly growing research area for the study of the protein cognate of genomic data. This review gives a brief overview of the modern proteomic technology. In addition to general applications of proteomics, we highlight its contribution to studying the physiology of different ocular tissues. We also summarise the published proteomic literature in the broad context of ophthalmic diseases, such as cataract, agerelated maculopathy, diabetic retinopathy, glaucoma and myopia. The proteomic technology is a useful research tool and it will continue to advance our understanding of a variety of molecular processes in ocular tissues and diseases.

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Identification and localization of major soluble vitreous proteins in human ocular tissue*1

Abstract: We present a normal human vitreous protein profile using current technologies and provide a baseline for comparison to ocular disease states. Tissue distribution of vitreous proteins may help to elucidate more specific protein function.

Cited by 3 publications

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Characterisation of the vitreous proteome in proliferative diabetic retinopathy

Characterisation of the vitreous proteome in proliferative diabetic retinopathy

Diet-induced obesity leads to pro-inflammatory alterations to the vitreous humour of the eye in a rat model

Application of proteomic technology in eye research: a mini review

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