Identification and localisation of selected myotropic neuropeptides in the ventral nerve cord of tenebrionid beetles

Marciniak, Paweł; Szymczak, Monika; Pacholska-Bogalska, Joanna; Audsley, Neil; Rosiński, Grzegorz

doi:10.1016/j.cbpa.2013.05.008

Cited by 13 publications

(17 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Proctolin has been identified in sensory, motor, and interneurons in the CNS of insects (Clark et al ., ). Immunofluorescent signals were identified in the brain and VNC of N. vespilloides , which confirmed results of studies on the beetles T. molitor , Z. atratus and L. decemlineata (Veenstra et al ., ; Breidbach & Dircksen, ; Marciniak et al ., ). Contrary to the results of a study by Veenstra et al .…”

Section: Discussioncontrasting

confidence: 69%

“…However, compared to results obtained by Marciniak et al . () and Schooneveld et al . (), more bilaterally placed perikaryons were observed.…”

Section: Discussionmentioning

confidence: 97%

“…Previous research on the localization of MS‐immunoreactive cells in the insect nervous system showed that these cells were present in different parts of CNS (Breidbach & Dircksen, ; Meola et al ., ; Nichols, ; Richer et al ., ; Söhler et al ., ; Marciniak et al ., ). However, despite positive results for the control of antibodies with the VNC of Z. atratus , we did not observe any immunolabeled cells in the N. vespilloides nervous system.…”

Section: Discussionmentioning

confidence: 97%

“…A control was performed by incubating fixed tissues in PBS with 0.4% Triton‐X 100, 2% NGS and 2% BSA without peptide antisera. Polyclonal rabbit antiproctolin (Novazym, previously used in Marciniak et al ., ), polyclonal rabbit anti‐MS (Novazym, previously used in Marciniak et al ., ), polyclonal rabbit anti‐Droso‐MIP (gift from Prof. J. A. Veenstra, previously used in Park et al ., ) and polyclonal rabbit anti‐Droso ‐ sNPF antibodies (gift from Prof. J.…”

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Myotropic activity and immunolocalization of selected neuropeptides of the burying beetle Nicrophorus vespilloides (Coleoptera: Silphidae)

et al. 2018

Self Cite

View full text Add to dashboard Cite

Burying beetles (Nicrophorus sp.) are necrophagous insects with developed parental care. Genome of Nicrophorus vespilloides has been recently sequenced, which makes them interesting model organism in behavioral ecology. However, we know very little about their physiology, including the functioning of their neuroendocrine system. In this study, one of the physiological activities of proctolin, myosuppressin (Nicve-MS), myoinhibitory peptide (Trica-MIP-5) and the short neuropeptide F (Nicve-sNPF) in N. vespilloides have been investigated. The tested neuropeptides were myoactive on N. vespilloides hindgut. After application of the proctolin increased hindgut contraction frequency was observed (EC value was 5.47 × 10 mol/L). The other tested neuropeptides led to inhibition of N. vespilloides hindgut contractions (Nicve-MS: IC = 5.20 × 10 mol/L; Trica-MIP-5: IC = 5.95 × 10 mol/L; Nicve-sNPF: IC = 4.08 × 10 mol/L). Moreover, the tested neuropeptides were immunolocalized in the nervous system of N. vespilloides. Neurons containing sNPF and MIP in brain and ventral nerve cord (VNC) were identified. Proctolin-immunolabeled neurons only in VNC were observed. Moreover, MIP-immunolabeled varicosities and fibers in retrocerebral complex were observed. In addition, our results have been supplemented with alignments of amino acid sequences of these neuropeptides in beetle species. This alignment analysis clearly showed amino acid sequence similarities between neuropeptides. Moreover, this allowed to deduce amino acid sequence of N. vespilloides proctolin (RYLPTa), Nicve-MS (QDVDHVFLRFa) and six isoforms of Nicve-MIP (Nicve-MIP-1-DWNRNLHSWa; Nicve-MIP-2-AWQNLQGGWa; Nicve-MIP-3-AWQNLQGGWa; Nicve-MIP-4-AWKNLNNAGWa; Nicve-MIP-5-SEWGNFRGSWa; Nicve-MIP-6- DPAWTNLKGIWa; and Nicve-sNPF-SGRSPSLRLRFa).

show abstract

Section: Discussioncontrasting

confidence: 69%

“…However, compared to results obtained by Marciniak et al . () and Schooneveld et al . (), more bilaterally placed perikaryons were observed.…”

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 97%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Myotropic activity and immunolocalization of selected neuropeptides of the burying beetle Nicrophorus vespilloides (Coleoptera: Silphidae)

et al. 2018

Self Cite

View full text Add to dashboard Cite

show abstract

“…This approach relies on the same high sensitivity, high throughput MS-based technology described above, but focuses on specific peptides [41, 42] or peptide families [43–45] that are usually implicated in biological pathways of interest to the researchers (Figure 2A). Identification of PTM sites on known peptides is another example of a targeted peptidomics approach [46].…”

Section: Choosing Your Peptidomics Modus Operandi: a Guide On Methodomentioning

confidence: 99%

Peptidomics for the discovery and characterization of neuropeptides and hormones

Romanova

Sweedler

2015

Trends in Pharmacological Sciences

View full text Add to dashboard Cite

The discovery of neuropeptides as signaling molecules with paracrine or hormonal regulatory functions has led to trailblazing advances in physiology and fostered the characterization of numerous neuropeptide-binding G-protein coupled receptors (GPCRs) as potential drug targets. The impact on human health has been tremendous: approximately 30% of commercial drugs act via the GPCR pathway. However, about 25% of the GPCRs encoded by the mammalian genome still lack their pharmacological identity. Searching for the orphan GPCR endogenous ligands that likely are neuropeptides has proved to be a formidable task. Here we describe the mass spectrometry-based technologies and experimental strategies that have been successful in achieving high throughput characterization of endogenous peptides in nervous and endocrine systems. Keywordsbioinformatics; endogenous peptides; sequencing; mass spectrometry; nervous system; quantitation What is peptidomics?Genome and transcriptome sequencing is upon us, so why we are still looking for ways to identify bioactive peptides in living systems? Bold genomic research efforts have provided extraordinary insights into the inventory of GPCR-receptor genes, the most coveted targets in drug development [1]. Gene association and knockout studies have illuminated the roles of various peptide genes in pathological conditions and diseases in animal models [2,3]. Yet genetic investigations do not determine the actual peptide gene products, neuropeptides, and hormones that mediate vital body functions and complex behaviors. The immense challenge is due to the complexity of molecular readout; a single gene can produce many products as a result of single nucleotide polymorphisms, alternative gene splicing, post-translational processing of precursor proteins, and the addition of chemical post-translational Corresponding author: Sweedler, J.V., jsweedle@illinois.edu. Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. HHS Public Access Author ManuscriptAuthor Manuscript Author ManuscriptAuthor Manuscript modifications (PTMs) of cleaved peptides that oftentimes cannot be inferred from genomic data. Even annotating peptide coding genes and in silico neuropeptidome prediction requires specialized expertise and bioinformatics tools [4][5][6].At the dawn of the new millennium, the term 'peptidomics' (see the workflow shown in Figure 1A) was formally adopted to describe a method for high throughput, direct measurement and structural characterization of the endogenous peptides present in a given biological sample (see detailed historical review by Schrader and co...

show abstract

A Mini-Review on Potential of Neuropeptides as Future Therapeutics

Sharma

Kumar

Bisht

2022

Int J Pept Res Ther

View full text Add to dashboard Cite

Identification and localisation of selected myotropic neuropeptides in the ventral nerve cord of tenebrionid beetles

Cited by 13 publications

References 46 publications

Myotropic activity and immunolocalization of selected neuropeptides of the burying beetle Nicrophorus vespilloides (Coleoptera: Silphidae)

Myotropic activity and immunolocalization of selected neuropeptides of the burying beetle Nicrophorus vespilloides (Coleoptera: Silphidae)

Peptidomics for the discovery and characterization of neuropeptides and hormones

A Mini-Review on Potential of Neuropeptides as Future Therapeutics

Contact Info

Product

Resources

About