Identification and intra-specific variability analysis of secreted and membrane-bound proteins from Echinococcus granulosus

Rosenzvit, Mara Cecília; Camicia, Federico; Kamenetzky, Laura; Muzulin, Patricia M.; Gutierrez, Ariana M.

doi:10.1016/j.parint.2005.11.009

Cited by 27 publications

(14 citation statements)

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“…The expression of different AgB subunits and their genomic variants may reflect an adaptive response of the parasite to different hosts and physiological conditions, diversifying AgB biochemical functions and antigenicity to promote its survival and evade host immune response [21], [33].…”

Section: Discussionmentioning

confidence: 99%

Echinococcus granulosus Antigen B Structure: Subunit Composition and Oligomeric States

et al. 2012

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BackgroundAntigen B (AgB) is the major protein secreted by the Echinococcus granulosus metacestode and is involved in key host-parasite interactions during infection. The full comprehension of AgB functions depends on the elucidation of several structural aspects that remain unknown, such as its subunit composition and oligomeric states.Methodology/Principal FindingsThe subunit composition of E. granulosus AgB oligomers from individual bovine and human cysts was assessed by mass spectrometry associated with electrophoretic analysis. AgB8/1, AgB8/2, AgB8/3 and AgB8/4 subunits were identified in all samples analyzed, and an AgB8/2 variant (AgB8/2v8) was found in one bovine sample. The exponentially modified protein abundance index (emPAI) was used to estimate the relative abundance of the AgB subunits, revealing that AgB8/1 subunit was relatively overrepresented in all samples. The abundance of AgB8/3 subunit varied between bovine and human cysts. The oligomeric states formed by E. granulosus AgB and recombinant subunits available, rAgB8/1, rAgB8/2 and rAgB8/3, were characterized by native PAGE, light scattering and microscopy. Recombinant subunits showed markedly distinct oligomerization behaviors, forming oligomers with a maximum size relation of rAgB8/3>rAgB8/2>rAgB8/1. Moreover, the oligomeric states formed by rAgB8/3 subunit were more similar to those observed for AgB purified from hydatid fluid. Pressure-induced dissociation experiments demonstrated that the molecular assemblies formed by the more aggregative subunits, rAgB8/2 and rAgB8/3, also display higher structural stability.Conclusions/SignificanceFor the first time, AgB subunit composition was analyzed in samples from single hydatid cysts, revealing qualitative and quantitative differences between samples. We showed that AgB oligomers are formed by different subunits, which have distinct abundances and oligomerization properties. Overall, our findings have significantly contributed to increase the current knowledge on AgB expression and structure, highlighting issues that may help to understand the parasite adaptive response during chronic infection.

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Section: Discussionmentioning

confidence: 99%

Echinococcus granulosus Antigen B Structure: Subunit Composition and Oligomeric States

et al. 2012

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“…Their finding is supported by Mamuti et al [6] who concluded that the genetic variability in different host and/or different level of host immune responses against AgB may be relevant factors in the variability that is observed in the immunogenecity of AgB among different CE patients. In addition, Rosenzvit et al [7] reported that analyzing the variation of AgB-coding genes may provide a specific correlation of strain genetic diversity with AgB functional and practical significance. The knowledge of variation in AgB genes would help the understanding of the fine mechanisms of adaptation of helminth parasites to their hosts and is necessary for the rational design and application of diagnostic tests, anthelmintic drugs, and immunoprophylaxis reagents.…”

Section: Discussionmentioning

confidence: 99%

“…Rosenzvit et al [7] suggested that precisely the contact with the host molecules and cells makes AgB proteins prone to antigenic variation which can affect their practical application. Therefore, serologic evaluation of AgB prepared from different E. granulosus hosts in endemic regions and countries according to local facilities must always be considered [6].…”

Section: Introductionmentioning

confidence: 99%

Genetic Variability of Antigen B among Echinococcus granulosus Egyptian Isolates

et al. 2009

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Genetic polymorphisms of encoding antigen B2 gene (AgB2) in Echinococcus granulosus were studied using PCR-RFLP and DNA sequencing among 20 Egyptian isolates. Five isolates from different host origins (humans, camels, pigs, and sheep) were collected and used. All examined isolates of each host group gave very similar patterns of PCR-RFLP after restriction enzyme digestion with AluI, with the gene size of approximately 140 bp and 240 bp for sheep and human isolates, and approximately 150 bp and 250 bp for pig and camel isolates. No digestion pattern was obtained after incubation of all studied isolates with EcoRI. These results reveal high intra-group homogeneity. DNA sequence analysis highlighted that human infecting strain showed 100% identity with respect to sheep infecting isolate, 96% and 99% with pig and camel infecting isolates, respectively.

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“…After establishment in the host, the hydatid cyst produces a large amount of AgB , which can alter the Th1/Th2 ratio from a predominant Th1 response in the early stage of infection to a Th2 response (Rigano et al, 2001), indicating that AgB is a modulator of the T cell response benefiting parasite survival. A number of studies have shown that AgB is encoded by a gene family and the antigen exhibits a high degree of genetic polymorphism (Haag et al, 2006;Rosenzvit et al, 2006;Kamenetzky et al, 2005), suggesting that the Echinococcus organisms have evolved antigenic variation-type mechanisms for escaping the host immune response.…”

Section: Regulatory T Cellsmentioning

confidence: 99%

Recent Advances in the Immunology and Serological Diagnosis of Echinococcosis

Zhang¹,

Li²,

Lin³

et al. 2012

Serological Diagnosis of Certain Human, Animal and Plant Diseases

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Identification and intra-specific variability analysis of secreted and membrane-bound proteins from Echinococcus granulosus

Cited by 27 publications

References 20 publications

Echinococcus granulosus Antigen B Structure: Subunit Composition and Oligomeric States

Echinococcus granulosus Antigen B Structure: Subunit Composition and Oligomeric States

Genetic Variability of Antigen B among Echinococcus granulosus Egyptian Isolates

Recent Advances in the Immunology and Serological Diagnosis of Echinococcosis

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