Identification and interaction of multiple genes resulting in DDT resistance in the 91-R strain of Drosophila melanogaster by RNAi approaches

Kim, Ju Hyeon; Moreau, Joseph A.; Zina, Jake M.; Mazgaeen, Lalita; Yoon, Kyong Sup; Pittendrigh, Barry R.; Clark, J. Marshall

doi:10.1016/j.pestbp.2018.03.003

Cited by 24 publications

(17 citation statements)

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“…Previous studies provide supporting evidence that high to moderate level DDT resistance is polygenic in Drosophila , with multiple resistance genes, including P450s, associated with the DDT resistance phenotype 8 – 10 . The Cyp6 subfamily has been associated with DDT resistance and cross-resistance to neonicotinoid insecticides 32 , 33 .…”

Section: Discussionmentioning

confidence: 80%

“…Specifically, we showed here that transcript from two cytochrome P450 genes, Cyp6g1 and Cyp6g2, are up-regulated in the DDT-resistant strain 91-R as compared to the -susceptible strains 91-C and Canton-S, reconfirming our previously published results 12,31 . Previous studies provide supporting evidence that high to moderate level DDT resistance is polygenic in Drosophila, with multiple resistance genes, including P450s, associated with the DDT resistance phenotype [8][9][10] . The Cyp6 subfamily has been associated with DDT resistance and cross-resistance to neonicotinoid insecticides 32,33 .…”

Section: Discussionmentioning

confidence: 80%

“…4 ), as well as evidence that other genes are functionally involved, such as the phase III detoxification ATP binding cassette transporter, Mdr49 13 . Furthermore, RNAi mediated knockdown of several phase I ( Cyp6g1 , Cyp12d1 , and Cyp4g1 ), phase III ( Mdr49 , Mdr50 , Mdr65 , and Mrp1 ), and other cuticular ( Lcp1 ) genes documented their capacity to contribute to DDT resistance in 91-R 8 . Moreover, DDT resistance in 91-R is dependent upon genetic factors located within 13 major and 3 minor genome regions affected by selective sweeps 14 , as well as changing in multiple pathways that impact neuronal function and cell stress response 31 .…”

Section: Discussionmentioning

confidence: 99%

“…The Drosophila model laboratory strain for DDT resistance, 91-R , has been under chronic exposure to DDT for over six decades and reared in parallel with the non-selected control strain 91-C ; the two strains came from a common population that was split before these decades-long difference in treatment of the two populations. This high-level DDT resistance phenotype in Drosophila is polygenic and associated with multimodal resistance mechanisms 8 , 9 including, but not limited to, involvement of phase I, II, and III detoxification enzymes. For example, the variance in protein structure and transcript expression of cytochrome P450 monooxygenases (P450s), including Cyp6g1 and ATP-binding cassette (ABC) transporters, has been reported in the DDT-resistant 91-R compared to DDT-susceptible strains 91-C and Canton-S 10 – 13 .…”

Section: Introductionmentioning

confidence: 99%

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Post-transcriptional modulation of cytochrome P450s, Cyp6g1 and Cyp6g2, by miR-310s cluster is associated with DDT-resistant Drosophila melanogaster strain 91-R

Seong

Coates

Pittendrigh

2020

Sci Rep

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The role of miRNAs in mediating insecticide resistance remains largely unknown, even for the model species Drosophila melanogaster. Building on prior research, this study used microinjection of synthetic miR-310s mimics into DDt-resistant 91-R flies and observed both a significant transcriptional repression of computationally-predicted endogenous target P450 detoxification genes, Cyp6g1 and Cyp6g2, and also a concomitant increase in DDT susceptibility. Additionally, co-transfection of D. melanogaster S2 cells with dual luciferase reporter constructs validated predictions that miR-310s bind to target binding sites in the 3ʹ untranslated regions (3ʹ-UtR) of both Cyp6g1 and Cyp6g2 in vitro. Findings in the current study provide empirical evidence for a link between reduced miRNA expression and an insecticidal resistance phenotype through reduced targeted post-transcriptional suppression of transcripts encoding proteins involved in xenobiotic detoxification. These insights are important for understanding the breadth of adaptive molecular changes that have contributed to the evolution of DDt resistance in D. melanogaster.

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Section: Discussionmentioning

confidence: 80%

Section: Discussionmentioning

confidence: 80%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Post-transcriptional modulation of cytochrome P450s, Cyp6g1 and Cyp6g2, by miR-310s cluster is associated with DDT-resistant Drosophila melanogaster strain 91-R

Seong

Coates

Pittendrigh

2020

Sci Rep

Self Cite

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“…Pesticide resistance is an ongoing problem for those tasked with pest management [1]. Resistance can occur through multiple mechanisms including target site insensitivity, reduced penetration, and sequestration as well as through changes in phase I, II, or III detoxification enzymes and transporters [2][3][4][5][6]. The chemical composition of the active toxin, including the pesticide class, level of exposure, duration of selection, genetic variation of the target species, and possibly other biotic and abiotic factors may also play roles in the evolution of resistance [1].…”

Section: Introductionmentioning

confidence: 99%

Dietary antioxidants impact DDT resistance in Drosophila melanogaster

et al. 2020

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Insects experience a diversity of subtoxic and/or toxic xenobiotics through exposure to pesticides and, in the case of herbivorous insects, through plant defensive compounds in their diets. Many insects are also concurrently exposed to antioxidants in their diets. The impact of dietary antioxidants on the toxicity of xenobiotics in insects is not well understood, in part due to the challenge of developing appropriate systems in which doses and exposure times (of both the antioxidants and the xenobiotics) can be controlled and outcomes can be easily measured. However, in Drosophila melanogaster, a well-established insect model system, both dietary factors and pesticide exposure can be easily controlled. Additionally, the mode of action and xenobiotic metabolism of dichlorodiphenyltrichloroethane (DDT), a highly persistent neurotoxic organochlorine insecticide that is detected widely in the environment, have been well studied in DDT-susceptible and-resistant strains. Using a glass-vial bioassay system with blue diet as the food source, seven compounds with known antioxidant effects (ascorbic acid, β-carotene, glutathione, α-lipoic acid, melatonin, minocycline, and serotonin) were orally tested for their impact on DDT toxicity across three strains of D. melanogaster: one highly susceptible to DDT (Canton-S), one mildly susceptible (91-C), and one highly resistant (91-R). Three of the antioxidants (serotonin, ascorbic acid, and β-carotene) significantly impacted the toxicity of DDT in one or more strains. Fly strain and gender, antioxidant type, and antioxidant dose all affected the relative toxicity of DDT. Our work demonstrates that dietary antioxidants can potentially alter the toxicity of a xenobiotic in an insect population.

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The development of abamectin resistance in Liriomyza trifolii and its contribution to thermotolerance

Wang,

Chang,

Gong

et al. 2024

Pest Management Science

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BACKGROUNDLiriomyza trifolii is an economically‐significant, invasive pest of horticultural and vegetable crops. The larvae form tunnels in foliage and hasten senescence and death. Outbreaks of L. trifolii often erupt in hot weather and are driven by thermotolerance; furthermore, the poor effectiveness of pesticides has made outbreaks more severe. But it is still unclear whether the development of insecticide tolerance will contribute to the thermotolerance in L. trifolii.RESULTSTo explore potential synergistic relationships between insecticide exposure and thermotolerance in L. trifolii, we firstly generated an abamectin‐resistant (AB‐R) strain. Then knockdown behavior, eclosion and survival rates and expression levels of genes encoding heat shock proteins (Hsps) in L. trifolii were examined in abamectin‐resistant (AB‐R) and abamectin‐susceptible (AB‐S) strains. Our results demonstrated that long‐term selection pressure for abamectin resistance made L. trifolii more prone to develop cross‐resistance to other insecticides containing similar ingredients. Furthermore, the AB‐R strain exhibited enhanced thermotolerance and possessed an elevated critical thermal maximum temperature, and upregulated expression levels of Hsps during heat stress.CONCLUSIONCollectively, our results indicate that thermal adaption in L. trifolii was accompanied by emerging abamectin resistance. This study provides a theoretical basis for investigating the synergistic or cross‐adaptive mechanisms that insects use to cope with adversity and demonstrates the complexity of insect adaptation to environmental and chemical stress.This article is protected by copyright. All rights reserved.

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Identification and interaction of multiple genes resulting in DDT resistance in the 91-R strain of Drosophila melanogaster by RNAi approaches

Cited by 24 publications

References 50 publications

Post-transcriptional modulation of cytochrome P450s, Cyp6g1 and Cyp6g2, by miR-310s cluster is associated with DDT-resistant Drosophila melanogaster strain 91-R

Post-transcriptional modulation of cytochrome P450s, Cyp6g1 and Cyp6g2, by miR-310s cluster is associated with DDT-resistant Drosophila melanogaster strain 91-R

Dietary antioxidants impact DDT resistance in Drosophila melanogaster

The development of abamectin resistance in Liriomyza trifolii and its contribution to thermotolerance

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