Identification and in vivo characterization of PpaA, a regulator of photosystem formation in Rhodobacter sphaeroides

Gomelsky, Larissa; Sram, Jakub P.; Moskvin, Oleg V.; Horne, Irene; Dodd, Helen N.; Pemberton, John; McEwan, Alastair G.; Kaplan, Samuel; Gomelsky, Mark

doi:10.1099/mic.0.25972-0

Cited by 49 publications

(62 citation statements)

References 58 publications

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“…The answer to this paradox is not obvious at this time. The expression pattern for ppaA, which is transcriptionally coupled to ppsR, is very interesting, because a recent study of ppaA function in R. sphaeroides appears to be consistent with the observation reported here (68). Nonetheless, this gene, which is immediately upstream of ppsR and transcriptionally coupled to ppsR, expression levels of which are low and constant, shows low aerobic expression but increased expression as cells are grown photosynthetically at decreasing light intensities.…”

Section: Fig 3 Expression Pattern Of Regulatory Genes Known To Be Isupporting

confidence: 59%

“…osp (64), ppsR (14,65), mgpS (66), ihfA (67), ihfB (67), prrA (49), and fnrL (16). On the other hand, prrB (8,10), prrC (25), appA (50), ppaA (68), and tspO (56) reveal significant changes in expression levels, depending on growth condition. Genes prrB (histidine kinase) and prrC (signal transducer), part of the cbb 3 regulon together with the response regulator PrrA, are expressed to a greater extent under aerobic conditions with the expression levels diminishing as cells are grown at progressively lower light intensities.…”

Section: Effect Of Oxygen and Light On R Sphaeroides Transcriptomementioning

confidence: 99%

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Effects of Oxygen and Light Intensity on Transcriptome Expression in Rhodobacter sphaeroides 2.4.1

Roh

Smith

Kaplan

2004

Journal of Biological Chemistry

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The roles of oxygen and light on the regulation of photosynthesis gene expression in Rhodobacter sphaeroides 2.4.1 have been well studied over the past 50 years. More recently, the effects of oxygen and light on gene regulation have been shown to involve the interacting redox chains present in R. sphaeroides under diverse growth conditions, and many of the redox carriers comprising these chains have been well studied. However, the expression patterns of those genes encoding these redox carriers, under aerobic and anaerobic photosynthetic growth, have been less well studied. Here, we provide a transcriptional analysis of many of the genes comprising the photosynthesis lifestyle, including genes corresponding to many of the known regulatory elements controlling the response of this organism to oxygen and light. The observed patterns of gene expression are evaluated and discussed in light of our knowledge of the physiology of R. sphaeroides under aerobic and photosynthetic growth conditions. Finally, this analysis has enabled to us go beyond the traditional patterns of gene expression associated with the photosynthesis lifestyle and to consider, for the first time, the full complement of genes responding to oxygen, and variations in light intensity when growing photosynthetically. The data provided here should be considered as a first step in enabling one to model electron flow in R. sphaeroides 2.4

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Section: Fig 3 Expression Pattern Of Regulatory Genes Known To Be Isupporting

confidence: 59%

Section: Effect Of Oxygen and Light On R Sphaeroides Transcriptomementioning

confidence: 99%

Effects of Oxygen and Light Intensity on Transcriptome Expression in Rhodobacter sphaeroides 2.4.1

Roh

Smith

Kaplan

2004

Journal of Biological Chemistry

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“…RNA extraction and quantitative reverse transcription-PCR (RT-PCR) was performed as described earlier (16,46). In brief, rifampin was added to R. sphaeroides cultures at the final concentration of 200 g ml Ϫ1 to halt transcription initiation.…”

Section: Methodsmentioning

confidence: 99%

“…The separation of PS genes into two clusters based on the dynamics of blue-light responses observed here lends further support to this hypothesis. One candidate for such regulator is PpaA (photopigment and puc activator protein [16]). The expression of the ppaA gene is repressed by PpsR by ϳ2.5-fold at 5 min of blue-light irradiation (Fig.…”

Section: Vol 186 2004mentioning

confidence: 99%

Responses of the Rhodobacter sphaeroides Transcriptome to Blue Light under Semiaerobic Conditions

et al. 2004

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. Transcriptome data suggest that the onset of repression is also mediated by the AppA-PpsR system, albeit via an apparently different sensory mechanism. Expression of several genes, whose products may participate in photooxidative damage defense, including deoxypyrimidine photolyase, glutathione peroxidase, and quinol oxidoreductases, was increased. Among the genes upregulated were genes encoding two factors: E and 38 . The consensus promoter sequences for these factors were predicted in the upstream sequences of numerous upregulated genes, suggesting that coordinated action of E and 38 is responsible for the upregulation. Based on the dynamics of upregulation, the anti-E factor ChrR or its putative upstream partner is proposed to be the primary sensor. The identified transcriptome responses provided a framework for deciphering blue-light-dependent signal transduction pathways in R. sphaeroides.

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“…The PrrB/PrrA two-component system senses the electron transport through the cbb3 oxidase and induces transcription of photosynthesis genes at very low oxygen tension or in the absence of oxygen (5,(10)(11)(12)(13). Furthermore, the FnrL protein activates some photosynthesis genes at low oxygen tension (13) and the PpaA regulator activates some photosynthesis genes under aerobic conditions (14). More recently CryB, a member of a newly described cryptochrome family (15), was shown to affect expression of photosynthesis genes in R. sphaeroides and to interact with AppA (16,17).…”

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Regulation of bacterial photosynthesis genes by the small noncoding RNA PcrZ

Mank

Berghoff

Hermanns

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

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The small RNA PcrZ (photosynthesis control RNA Z) of the facultative phototrophic bacterium Rhodobacter sphaeroides is induced upon a drop of oxygen tension with similar kinetics to those of genes for components of photosynthetic complexes. High expression of PcrZ depends on PrrA, the response regulator of the PrrB/ PrrA two-component system with a central role in redox regulation in R. sphaeroides. In addition the FnrL protein, an activator of some photosynthesis genes at low oxygen tension, is involved in redox-dependent expression of this small (s)RNA. Overexpression of full-length PcrZ in R. sphaeroides affects expression of a small subset of genes, most of them with a function in photosynthesis. Some mRNAs from the photosynthetic gene cluster were predicted to be putative PcrZ targets and results from an in vivo reporter system support these predictions. Our data reveal a negative effect of PcrZ on expression of its target mRNAs. Thus, PcrZ counteracts the redox-dependent induction of photosynthesis genes, which is mediated by protein regulators. Because PrrA directly activates photosynthesis genes and at the same time PcrZ, which negatively affects photosynthesis gene expression, this is one of the rare cases of an incoherent feed-forward loop including an sRNA. Our data identified PcrZ as a trans acting sRNA with a direct regulatory function in formation of photosynthetic complexes and provide a model for the control of photosynthesis gene expression by a regulatory network consisting of proteins and a small noncoding RNA.α-proteobacteria | protochlorophyllide reductase

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Identification and in vivo characterization of PpaA, a regulator of photosystem formation in Rhodobacter sphaeroides

Cited by 49 publications

References 58 publications

Effects of Oxygen and Light Intensity on Transcriptome Expression in Rhodobacter sphaeroides 2.4.1

Effects of Oxygen and Light Intensity on Transcriptome Expression in Rhodobacter sphaeroides 2.4.1

Responses of the Rhodobacter sphaeroides Transcriptome to Blue Light under Semiaerobic Conditions

Regulation of bacterial photosynthesis genes by the small noncoding RNA PcrZ

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